JZUS - Journal of Zhejiang University SCIENCE

Journal of Zhejiang University SCIENCE B

Accepted manuscript available online (unedited version)

USP11 regulates p53 stability by deubiquitinating p53

Author(s): Jia-ying Ke^1;2, Cong-jie Dai^1;2, Wen-lin Wu^1;2, Jin-hua Gao³, Ai-juan Xia⁴, Guang-ping Liu⁵, Kao-sheng Lv⁶, Chun-lin Wu⁷
Affiliation(s): 1. College of Chemistry and Life Science, Quanzhou Normal University, Quanzhou 36200, China;2. The Higher Educational Key Laboratory for Molecular Biology and Pharmacology of Fujian Province, Quanzhou 36200, China;3. Xiamen Women and Children Health Hospital, Xiamen 361005, China;4. Shouguang Peoples Hospital, Shouguang 262700, China;5. Department of Orthopedics, Central Hospital of Zibo, Zibo 255000, China;6. Department of Pathology, University of Chicago, Chicago 60102, Illinois, USA;7. Department of Pathology, the Second Affiliated Hospital of Fujian Medical University, Quanzhou 36200, China
Corresponding email(s): kejiaying2003@163.com
Key Words: p53, USP11, Deubiquitination, Stability

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Jia-ying Ke, Cong-jie Dai, Wen-lin Wu, Jin-hua Gao, Ai-juan Xia, Guang-ping Liu, Kao-sheng Lv, Chun-lin Wu. USP11 regulates p53 stability by deubiquitinating p53[J]. Journal of Zhejiang University Science B,in press.Frontiers of Information Technology & Electronic Engineering,in press.https://doi.org/10.1631/jzus.B1400180

@article{title="USP11 regulates p53 stability by deubiquitinating p53",
author="Jia-ying Ke, Cong-jie Dai, Wen-lin Wu, Jin-hua Gao, Ai-juan Xia, Guang-ping Liu, Kao-sheng Lv, Chun-lin Wu",
journal="Journal of Zhejiang University Science B",
year="in press",
publisher="Zhejiang University Press & Springer",
doi="https://doi.org/10.1631/jzus.B1400180"
}

%0 Journal Article
%T USP11 regulates p53 stability by deubiquitinating p53
%A Jia-ying Ke
%A Cong-jie Dai
%A Wen-lin Wu
%A Jin-hua Gao
%A Ai-juan Xia
%A Guang-ping Liu
%A Kao-sheng Lv
%A Chun-lin Wu
%J Journal of Zhejiang University SCIENCE B
%P 1032-1038
%@ 1673-1581
%D in press
%I Zhejiang University Press & Springer
doi="https://doi.org/10.1631/jzus.B1400180"

TY - JOUR
T1 - USP11 regulates p53 stability by deubiquitinating p53
A1 - Jia-ying Ke
A1 - Cong-jie Dai
A1 - Wen-lin Wu
A1 - Jin-hua Gao
A1 - Ai-juan Xia
A1 - Guang-ping Liu
A1 - Kao-sheng Lv
A1 - Chun-lin Wu
J0 - Journal of Zhejiang University Science B
SP - 1032
EP - 1038
%@ 1673-1581
Y1 - in press
PB - Zhejiang University Press & Springer
ER -
doi="https://doi.org/10.1631/jzus.B1400180"

Abstract
Chinese Summary
Academic Network
Reviewer Comment

Abstract: The p53 tumor suppressor protein coordinates the cellular responses to a broad range of cellular stresses, leading to DNA repair, cell cycle arrest or apoptosis. The stability of p53 is essential for its tumor suppressor function, which is tightly controlled by ubiquitin-dependent degradation primarily through its negative regulator murine double minute 2 (Mdm2). To better understand the regulation of p53, we tested the interaction between p53 and USP11 using co-immunoprecipitation. The results show that USP11, an ubiquitin-specific protease, forms specific complexes with p53 and stabilizes p53 by deubiquitinating it. Moreover, down-regulation of USP11 dramatically attenuated p53 induction in response to DNA damage stress. These findings reveal that USP11 is a novel regulator of p53, which is required for p53 activation in response to DNA damage.

USP11通过去泛素化p53调控p53稳定性

深入研究p53的泛素化及稳定性的调控。发现一个新的调控p53去泛素化的酶USP11，它可以通过与p53的结合去泛素化并稳定p53，从而揭示了一个新的p53去泛素化调控的机制。通过免疫共沉淀发现p53可以与USP11结合（图1a），通过泛素化检测试验发现USP11可以去泛素化p53（图3a和3b），最后通过逆转录-聚合酶链式反应（RT-PCR）试验发现在DNA损伤后，USP11对p53转录活性的提高是非常重要的。 USP11可通过去泛素化p53来调控p53稳定性。
p53基因；USP11；泛素化；稳定性

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USP11通过去泛素化p53调控p53稳定性

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References