Abstract: Syzygium campanulatum Korth is a plant, which is a rich source of secondary metabolites (especially flavanones, chalcone, and triterpenoids). In our present study, three conventional solvent extraction (CSE) techniques and supercritical fluid extraction (SFE) techniques were performed to achieve a maximum recovery of two flavanones, chalcone, and two triterpenoids from S. campanulatum leaves. Furthermore, a Box-Behnken design was constructed for the SFE technique using pressure, temperature, and particle size as independent variables, and yields of crude extract, individual and total secondary metabolites as the dependent variables. In the CSE procedure, twenty extracts were produced using ten different solvents and three techniques (maceration, soxhletion, and reflux). An enriched extract of five secondary metabolites was collected using n-hexane:methanol (1:1) soxhletion. Using food-grade ethanol as a modifier, the SFE methods produced a higher recovery (25.5%‒84.9%) of selected secondary metabolites as compared to the CSE techniques (0.92%‒66.00%).

传统溶剂提取法和超临界流体萃取法对钟花蒲桃次生代谢产物的回收率的比较研究

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[18]List of electronic supplementary materials

[19]Fig. S1 Representative HPLC chromatograms of other 14 CSE extracts from S. campanulatum

[20]Fig. S2 Representative HPLC chromatograms of lab-scale and pilot-scale SFE extracts from S. campanulatum at different pressures, temperatures, and particle sizes