JZUS - Journal of Zhejiang University SCIENCE

Journal of Zhejiang University SCIENCE B

Accepted manuscript available online (unedited version)

The protective effect of folic acid on oxidative stress induced by lead acetate in PC12 cells

Author(s): Ning LI1, Mingming LI, Xianqing HUANG, Lianjun SONG, Mingwu QIAO, Tianlin WANG, Tiange LI, Xiuhong ZHU, Can CUI
Affiliation(s): College of Food Science and Technology, Henan Agricultural University, Zhengzhou 450002, China; more
Corresponding email(s): ln8028@163.com, ningli@henau.edu.cn, slj69@126.com
Key Words: Lead; Folic acid; PC12 cells; Oxidative stress; Apoptosis

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Ning LI1, Mingming LI, Xianqing HUANG, Lianjun SONG, Mingwu QIAO, Tianlin WANG, Tiange LI, Xiuhong ZHU, Can CUI. The protective effect of folic acid on oxidative stress induced by lead acetate in PC12 cells[J]. Journal of Zhejiang University Science B,in press.Frontiers of Information Technology & Electronic Engineering,in press.https://doi.org/10.1631/jzus.B2100938

@article{title="The protective effect of folic acid on oxidative stress induced by lead acetate in PC12 cells",
author="Ning LI1, Mingming LI, Xianqing HUANG, Lianjun SONG, Mingwu QIAO, Tianlin WANG, Tiange LI, Xiuhong ZHU, Can CUI",
journal="Journal of Zhejiang University Science B",
year="in press",
publisher="Zhejiang University Press & Springer",
doi="https://doi.org/10.1631/jzus.B2100938"
}

%0 Journal Article
%T The protective effect of folic acid on oxidative stress induced by lead acetate in PC12 cells
%A Ning LI1
%A Mingming LI
%A Xianqing HUANG
%A Lianjun SONG
%A Mingwu QIAO
%A Tianlin WANG
%A Tiange LI
%A Xiuhong ZHU
%A Can CUI
%J Journal of Zhejiang University SCIENCE B
%P
%@ 1673-1581
%D in press
%I Zhejiang University Press & Springer
doi="https://doi.org/10.1631/jzus.B2100938"

TY - JOUR
T1 - The protective effect of folic acid on oxidative stress induced by lead acetate in PC12 cells
A1 - Ning LI1
A1 - Mingming LI
A1 - Xianqing HUANG
A1 - Lianjun SONG
A1 - Mingwu QIAO
A1 - Tianlin WANG
A1 - Tiange LI
A1 - Xiuhong ZHU
A1 - Can CUI
J0 - Journal of Zhejiang University Science B
SP -
EP -
%@ 1673-1581
Y1 - in press
PB - Zhejiang University Press & Springer
ER -
doi="https://doi.org/10.1631/jzus.B2100938"

Abstract
Chinese Summary
Academic Network
Reviewer Comment

Abstract: Folic acid (FA) plays an important role in cell metabolism. In this study, we primarily looked at the protective effect of FA on PC12 cytotoxicity induced by lead acetate. PC12 cells were exposed to lead-free medium and 10μmol/L lead-acetate culture solution for 24h. Medium with FA proportions of 0mg/L, 0.5mg/L, 5mg/L, and 50mg/L continued to function. After 24h, 48h, and 72h, we collected PC12 cells, and detected cell proliferation or inhibition with the MTT method. We extracted cell protein and detected the activities of SOD, MDA, and GSH-PX in each group. The immunofluorescence method was used to study the expression of caspase-3 protein. Changes of Aβ₄₀ and Aβ₄₂ in the cell-culture fluid were detected with ELISA. The results showed that lead exposure inhibited cell growth (P < 0.05), increased intracellular MDA content, SOD activity, and caspase-3 protein expression (P < 0.05), and decreased GSH-PX activity and Aβ₄₀ protein expression (P < 0.05). FA intervention can increase cell proliferation, enhance the activity of SOD and GSH-PX (P < 0.05), and reduce cell MDA content and caspase-3 protein expression (P < 0.05). There was no statistically significant difference between Aβ₄₀ and Aβ₄₂ content between the groups (P > 0.05). The study found that FA could be a highly effective approach against lead-associated PC12 cell toxicity.

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