JZUS - Journal of Zhejiang University SCIENCE

Journal of Zhejiang University SCIENCE B

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Fucoidan sulfate from Sargassum fusiforme regulates the SARS-CoV-2 receptor AXL expression in human embryonic lung diploid fibroblast cells

Author(s): Xuqiang ZHOU, Weihua JIN, Di JIANG, Yipeng XU, Sanying WANG, Xinna WU, Yunchuang CHANG, Huili SU, Tianjun ZHU, Xiaogang XU, Genxiang MAO
Affiliation(s): College of Life Science, Zhejiang Chinese Medical University, Hangzhou 310053, China; more
Corresponding email(s): xuxg@zju.edu.cn, maogenxiang@163.com
Key Words: Senescent cell; Human embryonic lung diploid fibroblast cells; Fucoidan sulfate; AXL; Gene expression

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Xuqiang ZHOU, Weihua JIN, Di JIANG, Yipeng XU, Sanying WANG, Xinna WU, Yunchuang CHANG, Huili SU, Tianjun ZHU, Xiaogang XU, Genxiang MAO. Fucoidan sulfate from Sargassum fusiforme regulates the SARS-CoV-2 receptor AXL expression in human embryonic lung diploid fibroblast cells[J]. Journal of Zhejiang University Science B,in press.Frontiers of Information Technology & Electronic Engineering,in press.https://doi.org/10.1631/jzus.B2300087

@article{title="Fucoidan sulfate from Sargassum fusiforme regulates the SARS-CoV-2 receptor AXL expression in human embryonic lung diploid fibroblast cells",
author="Xuqiang ZHOU, Weihua JIN, Di JIANG, Yipeng XU, Sanying WANG, Xinna WU, Yunchuang CHANG, Huili SU, Tianjun ZHU, Xiaogang XU, Genxiang MAO",
journal="Journal of Zhejiang University Science B",
year="in press",
publisher="Zhejiang University Press & Springer",
doi="https://doi.org/10.1631/jzus.B2300087"
}

%0 Journal Article
%T Fucoidan sulfate from Sargassum fusiforme regulates the SARS-CoV-2 receptor AXL expression in human embryonic lung diploid fibroblast cells
%A Xuqiang ZHOU
%A Weihua JIN
%A Di JIANG
%A Yipeng XU
%A Sanying WANG
%A Xinna WU
%A Yunchuang CHANG
%A Huili SU
%A Tianjun ZHU
%A Xiaogang XU
%A Genxiang MAO
%J Journal of Zhejiang University SCIENCE B
%P 1047-1052
%@ 1673-1581
%D in press
%I Zhejiang University Press & Springer
doi="https://doi.org/10.1631/jzus.B2300087"

TY - JOUR
T1 - Fucoidan sulfate from Sargassum fusiforme regulates the SARS-CoV-2 receptor AXL expression in human embryonic lung diploid fibroblast cells
A1 - Xuqiang ZHOU
A1 - Weihua JIN
A1 - Di JIANG
A1 - Yipeng XU
A1 - Sanying WANG
A1 - Xinna WU
A1 - Yunchuang CHANG
A1 - Huili SU
A1 - Tianjun ZHU
A1 - Xiaogang XU
A1 - Genxiang MAO
J0 - Journal of Zhejiang University Science B
SP - 1047
EP - 1052
%@ 1673-1581
Y1 - in press
PB - Zhejiang University Press & Springer
ER -
doi="https://doi.org/10.1631/jzus.B2300087"

Abstract
Chinese Summary
Academic Network
Reviewer Comment

Abstract: Coronavirus disease 2019 (COVID-19) is a global infectious disease that seriously endangers human life and health and affects normal social activities. Since the pandemic outbreak from December 2019 to February 2023, the total number of confirmed cases has exceeded 753 million, and the deaths caused by COVID-19 have reached 6.6 million (https://covid19.who.int; accessed on Feb. 16, 2023). Currently, supportive therapy is the primary treatment for patients, as some antiviral drugs have demonstrated significant side effects during clinical treatment. Although vaccination has a particular effect on the prevention of novel coronavirus infection and the emergence of severe diseases, the variation in the virus strain caused the vaccine’s protective effect to be greatly weakened or lost. Therefore, there is an urgent need to identify safe and effective drugs to prevent and cure COVID-19. Relevant research and exploration can also provide a reference for drug screening with which to address similar infectious diseases and public health events in the future.

羊栖菜来源的褐藻多糖硫酸酯调控人胚肺二倍体成纤维细胞中新冠病毒受体分子AXL的表达

周叙强¹，金维华³，江迪³，徐一鹏⁴，王三应²，吴辛娜⁵，常允闯⁶，苏慧丽²，朱田君²，徐小刚²，毛根祥¹
¹浙江中医药大学生命科学学院，中国杭州市，310053
²浙江医院，浙江省老年医学重点实验室和浙江省老年医学研究所，中国杭州市，310030
³浙江工业大学生物工程学院，中国杭州市，310014
⁴浙江省肿瘤医院，浙江省核酸适体与临床诊治重点实验室，中国科学院基础医学与肿瘤研究所，中国杭州市，310022
⁵浙江大学医学院，中国杭州市，310058
⁶浙江科技学院生物与化学工程学院，中国杭州市，310023
摘要：新冠病毒感染疫情严重威胁着世界各国人民的生命健康。目前，对病毒感染的防治研究主要集中在抑制病毒与分子受体的结合上。AXL作为新发现的严重急性呼吸综合征冠状病毒2型（SARS-CoV-2）受体，在协助病毒感染人体呼吸系统中发挥着重要作用，是未来临床干预的潜在靶点。本研究对已发表的单细胞测序数据进行整理和分析，发现AXL在年轻人肺细胞中的表达水平明显高于老年人。人胚肺二倍体成纤维细胞（2BS）是衰老研究的公认细胞株。本文采用2BS细胞构建复制性细胞衰老模型，发现年轻细胞中AXL的蛋白水平明显高于衰老细胞，据此推测年轻人感染的风险可能更高，需要注意防护。我们发现一种羊栖菜褐藻多糖硫酸酯组分（SFW-3）可显著下调年轻2BS细胞中AXL的表达水平，表明SFW-3具有一定的抗SARS-CoV-2感染的研究价值，同时表明2BS细胞株也可作为潜在的SARS-CoV-2体外感染模型。

关键词组：新冠病毒；褐藻多糖硫酸酯；酪氨酸蛋白激酶受体；血管紧张素转化酶2；年龄

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羊栖菜来源的褐藻多糖硫酸酯调控人胚肺二倍体成纤维细胞中新冠病毒受体分子AXL的表达

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