JZUS - Journal of Zhejiang University SCIENCE

Journal of Zhejiang University SCIENCE B

Accepted manuscript available online (unedited version)

Co-culture of natural killer cells and tumor spheroids on a heterogeneous multilayer paper stack

Author(s): Yuanyuan XIE, Xiaoyan YANG, Rong PAN, Lixia GAO, Ling YU
Affiliation(s): Key Laboratory of Luminescence Analysis and Molecular Sensing, Ministry of Education, Institute for Clean Energy and Advanced Materials, School of Materials and Energy, Southwest University, Chongqing 400715, China; more
Corresponding email(s): lingyu12@swu.edu.cn
Key Words: Multilayer paper stack; Co-culture; Tumor spheroids; HUVEC; NK-92, Migration

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Yuanyuan XIE, Xiaoyan YANG, Rong PAN, Lixia GAO, Ling YU. Co-culture of natural killer cells and tumor spheroids on a heterogeneous multilayer paper stack[J]. Journal of Zhejiang University Science B,in press.Frontiers of Information Technology & Electronic Engineering,in press.https://doi.org/10.1631/jzus.B2300617

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author="Yuanyuan XIE, Xiaoyan YANG, Rong PAN, Lixia GAO, Ling YU",
journal="Journal of Zhejiang University Science B",
year="in press",
publisher="Zhejiang University Press & Springer",
doi="https://doi.org/10.1631/jzus.B2300617"
}

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%T Co-culture of natural killer cells and tumor spheroids on a heterogeneous multilayer paper stack
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%A Rong PAN
%A Lixia GAO
%A Ling YU
%J Journal of Zhejiang University SCIENCE B
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doi="https://doi.org/10.1631/jzus.B2300617"

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A1 - Yuanyuan XIE
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A1 - Rong PAN
A1 - Lixia GAO
A1 - Ling YU
J0 - Journal of Zhejiang University Science B
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%@ 1673-1581
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PB - Zhejiang University Press & Springer
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doi="https://doi.org/10.1631/jzus.B2300617"

Abstract
Chinese Summary
Academic Network
Reviewer Comment

Abstract: Prostate cancer is one of the malignant tumors endangering human life. Multilayered paper-based cell culture, as an in vitro three-dimensional (3D) cell culture method, has been frequently used to research drug bioavailability, therapeutic efficacy and dose-limiting toxicity in malignant tumors. This paper proposes a heterogenous multilayer paper stacking co-culture system to establish a model of natural killer cells (NKs) from the endothelium layer, which attack tumor spheroids. This system consists of three layers: a bottom tumor spheroids layer, a middle invasion layer, and a top endothelium layer. NK-92 cells were placed in the supernatant on top of the three layers. After two days of co-culture, the attack of tumor spheroids by NKs was observed. We additionally examined the infiltration of NK-92 cells within the tumor spheroids at different Z-axis depths using a confocal microscope, and the results suggested that the system successfully realizes NKs traveling cross the endothelium layer to form tumor infiltrating NKs (TINKs). The potential application of multilayered papers for co-culture models involving cancer cells and immune cells holds great promise for exploring the interaction dynamics of these two cell types.

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