JZUS - Journal of Zhejiang University SCIENCE

Journal of Zhejiang University SCIENCE B

Accepted manuscript available online (unedited version)

Metagenomic sequencing reveals high reproducibility of human donor microbiota transplanted into germ-free mice via lower gut route

Author(s): Yapeng YANG^1, ^2, ³, Xiang TAN², Zeyue ZHANG², Lifeng LIANG⁴, Zhifeng WU², Jinhui HE², Yuqing WANG^2, ³, Miaomiao DONG², Jixia ZHENG², Hang ZHANG^2, ⁵, Shuaifei FENG², Wei CHENG², Bota CUI⁶, Hong WEI^2, ⁵, Qinjin LI¹
Affiliation(s): ¹College of Animal Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China; more
Corresponding email(s): liqinjin0809@163.com, weihong63528@163.com
Key Words: Fecal microbiota transplantation; Germ-free mice; Lower gut; Gavage; Metagenome

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Yapeng YANG^1,^2,³, Xiang TAN², Zeyue ZHANG², Lifeng LIANG⁴, Zhifeng WU², Jinhui HE², Yuqing WANG^2,³, Miaomiao DONG², Jixia ZHENG², Hang ZHANG^2,⁵, Shuaifei FENG², Wei CHENG², Bota CUI⁶, Hong WEI^2,⁵, Qinjin LI¹. Metagenomic sequencing reveals high reproducibility of human donor microbiota transplanted into germ-free mice via lower gut route[J]. Journal of Zhejiang University Science B,in press.Frontiers of Information Technology & Electronic Engineering,in press.https://doi.org/10.1631/jzus.B2400495

@article{title="Metagenomic sequencing reveals high reproducibility of human donor microbiota transplanted into germ-free mice via lower gut route",
author="Yapeng YANG^1,^2,³, Xiang TAN², Zeyue ZHANG², Lifeng LIANG⁴, Zhifeng WU², Jinhui HE², Yuqing WANG^2,³, Miaomiao DONG², Jixia ZHENG², Hang ZHANG^2,⁵, Shuaifei FENG², Wei CHENG², Bota CUI⁶, Hong WEI^2,⁵, Qinjin LI¹",
journal="Journal of Zhejiang University Science B",
year="in press",
publisher="Zhejiang University Press & Springer",
doi="https://doi.org/10.1631/jzus.B2400495"
}

%0 Journal Article
%T Metagenomic sequencing reveals high reproducibility of human donor microbiota transplanted into germ-free mice via lower gut route
%A Yapeng YANG^1
%A^2
%A³
%A Xiang TAN²
%A Zeyue ZHANG²
%A Lifeng LIANG⁴
%A Zhifeng WU²
%A Jinhui HE²
%A Yuqing WANG^2
%A³
%A Miaomiao DONG²
%A Jixia ZHENG²
%A Hang ZHANG^2
%A⁵
%A Shuaifei FENG²
%A Wei CHENG²
%A Bota CUI⁶
%A Hong WEI^2
%A⁵
%A Qinjin LI¹
%J Journal of Zhejiang University SCIENCE B
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%@ 1673-1581
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doi="https://doi.org/10.1631/jzus.B2400495"

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A1 - Miaomiao DONG²
A1 - Jixia ZHENG²
A1 - Hang ZHANG^{2
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doi="https://doi.org/10.1631/jzus.B2400495"

Abstract
Chinese Summary
Academic Network
Reviewer Comment

Abstract: Human flora-associated (HFA) mice are often used to simulate the structure of human intestinal microbiota and to study the causal relationship between diseases and gut microbiota. However, several factors affect the colonization efficiency of human microbiota in germ-free (GF) mice, and the differential effects of gavage and lower gut transplantation on colonization are still unclear. In this study, we explored the reproducibility of recipient-to-donor gut microbiota community structure and function under different transplantation routes and the differences in microbial colonization between recipients via gavage (GT_mice group) and the lower gut (LGT_mice group). High-throughput sequencing of the metagenome was performed on the feces of each subject, and the composition of mi-crobiome of each group was analyzed. As expected, the introduction of human fecal microbiota into GF mice via lower gut trans-plantation had a high transfer efficiency, which was evident from the similar species community structure to the donor (Adonis R², LGT_mice group – Donor group = 0.71396; GT_mice group – Donor group = 0.774095) and the higher bacterial colonization rate. The findings provide unique insights into improving the accuracy of constructing humanized microbiota transplantation models, aiding our understanding of the relationships between the human gut microbiota and disease.

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