JZUS - Journal of Zhejiang University SCIENCE

Journal of Zhejiang University SCIENCE B

Accepted manuscript available online (unedited version)

Polarizing macrophages derived from human THP-1 cells in vitro: methods and protocols

Author(s): Pengfei LI, Lin CHEN, Wei YUAN, Xingqiang LI, Xuesong FENG
Affiliation(s): Laboratory of Animal Center, Medical Experiment Center, Shaanxi University of Chinese Medicine, Xianyang 712046, China; more
Corresponding email(s): 2111051@sntcm.edu.cn
Key Words: THP-1 macrophage; Polarization; Method; Marker

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Pengfei LI, Lin CHEN, Wei YUAN, Xingqiang LI, Xuesong FENG. Polarizing macrophages derived from human THP-1 cells in vitro: methods and protocols[J]. Journal of Zhejiang University Science B,in press.Frontiers of Information Technology & Electronic Engineering,in press.https://doi.org/10.1631/jzus.B2400516

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author="Pengfei LI, Lin CHEN, Wei YUAN, Xingqiang LI, Xuesong FENG",
journal="Journal of Zhejiang University Science B",
year="in press",
publisher="Zhejiang University Press & Springer",
doi="https://doi.org/10.1631/jzus.B2400516"
}

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%T Polarizing macrophages derived from human THP-1 cells in vitro: methods and protocols
%A Pengfei LI
%A Lin CHEN
%A Wei YUAN
%A Xingqiang LI
%A Xuesong FENG
%J Journal of Zhejiang University SCIENCE B
%P 1132-1136
%@ 1673-1581
%D in press
%I Zhejiang University Press & Springer
doi="https://doi.org/10.1631/jzus.B2400516"

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T1 - Polarizing macrophages derived from human THP-1 cells in vitro: methods and protocols
A1 - Pengfei LI
A1 - Lin CHEN
A1 - Wei YUAN
A1 - Xingqiang LI
A1 - Xuesong FENG
J0 - Journal of Zhejiang University Science B
SP - 1132
EP - 1136
%@ 1673-1581
Y1 - in press
PB - Zhejiang University Press & Springer
ER -
doi="https://doi.org/10.1631/jzus.B2400516"

Abstract
Chinese Summary
Academic Network
Reviewer Comment

Abstract: Macrophages derived from the human THP-1 cell line have been widely used as substitutes for primary macrophages in various macrophage-related studies. However, difficulties still exist in establishing THP-1 macrophage models. This research presents techniques for generating different phenotypes of activated macrophages derived from THP-1 cells by introducing specific stimuli and provides some potential markers to confirm each type of activated macrophage. It is hoped to provide novel and useful methods for scientific research and to help researchers explore this field more intuitively and effectively.

体外诱导人THP-1细胞源性巨噬细胞极化：方法与流程

李鹏飞^1,2，陈琳³，袁伟¹，李兴强²，冯雪松^1,2
¹陕西中医药大学医学科研实验中心，动物中心实验室，中国咸阳市，712046
²陕西中医药大学基础医学院，中国咸阳市，712046
³第四军医大学西京医院心内科，中国西安市，710032
摘要：巨噬细胞具有高度的可塑性与异质性，能在特定微环境中调控细胞增殖、介导免疫调节并促进组织再生。在相关研究中，由人源THP-1细胞系诱导分化而来的巨噬细胞，已被广泛用作原代巨噬细胞的替代模型。然而，在诱导建立THP-1巨噬细胞的各类模型时，仍面临诸多挑战。本研究详细阐述了通过特定刺激将THP-1细胞诱导为不同表型巨噬细胞的方法，并筛选出一系列可用于识别不同巨噬细胞表型的潜在标志物，旨在为该领域研究提供新颖且实用的技术手段，助力研究人员更直观、高效地开展探索。

关键词组：THP-1巨噬细胞；极化方法；表型；标志物

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Reference

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体外诱导人THP-1细胞源性巨噬细胞极化：方法与流程

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