JZUS - Journal of Zhejiang University SCIENCE

Journal of Zhejiang University SCIENCE B

Accepted manuscript available online (unedited version)

Ectopic expression of structurally similar major royal jelly proteins reveals their distinct functions in Drosophila

Author(s): Lingqi YU¹, Danfeng WANG^1, ³, Xuanhao CHEN¹, Jiayu XIE⁴, Dongjing WEN¹, Yi ZHANG¹, Lirong SHEN⁵, Wenfeng CHEN^1, ², Zhenxing LIU⁶, Yufeng YANG^1, ²
Affiliation(s): ¹Institute of Life Sciences, College of Biological Science and Engineering, Fuzhou University, Fuzhou 350108, China; more
Corresponding email(s): chenwenfeng@fzu.edu.cn, liuzhenxing01@caas.cn, yangyf@fzu.edu.cn
Key Words: Drosophila melanogaster; Major royal jelly proteins; Ectopic expression

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Lingqi YU¹, Danfeng WANG^1,³, Xuanhao CHEN¹, Jiayu XIE⁴, Dongjing WEN¹, Yi ZHANG¹, Lirong SHEN⁵, Wenfeng CHEN^1,², Zhenxing LIU⁶, Yufeng YANG^1,². Ectopic expression of structurally similar major royal jelly proteins reveals their distinct functions in Drosophila[J]. Journal of Zhejiang University Science B,in press.Frontiers of Information Technology & Electronic Engineering,in press.https://doi.org/10.1631/jzus.B2400624

@article{title="Ectopic expression of structurally similar major royal jelly proteins reveals their distinct functions in Drosophila",
author="Lingqi YU¹, Danfeng WANG^1,³, Xuanhao CHEN¹, Jiayu XIE⁴, Dongjing WEN¹, Yi ZHANG¹, Lirong SHEN⁵, Wenfeng CHEN^1,², Zhenxing LIU⁶, Yufeng YANG^1,²",
journal="Journal of Zhejiang University Science B",
year="in press",
publisher="Zhejiang University Press & Springer",
doi="https://doi.org/10.1631/jzus.B2400624"
}

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%A Zhenxing LIU⁶
%A Yufeng YANG^1
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%J Journal of Zhejiang University SCIENCE B
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Abstract
Chinese Summary
Academic Network
Reviewer Comment

Abstract: Royal jelly (RJ), secreted by the hypopharyngeal and mandibular glands of young worker bees, is rich in proteins, 80%-90% of which are major royal jelly proteins (MRJPs). While MRJPs from RJ have been shown to exhibit specific biological functions and are also expressed in neuronal cells, their roles in vivo remain poorly understood. The aim of this study was to elucidate the functional roles of individual MRJPs (MRJP1-9) in vivo by ectopically expressing them in Drosophila neurons in a binary expression system using fluorescent proteins as controls. Transcriptome sequencing revealed that although MRJP1-9 share similar tertiary structures, their overexpression affects distinct gene sets. MRJP1, MRJP2, MRJP3, MRJP5, and MRJP7 induced more differentially expressed genes, while MRJP4, MRJP6, MRJP8, and MRJP9 induced fewer such genes. Weighted gene co-expression network analysis (WGCNA) and gene set enrichment analysis (GSEA) revealed that MRJP1, MRJP2, MRJP3, MRJP5, and MRJP7 regulate overlapping gene sets, including an estradiol-responsive set, and activate cell proliferation pathways. MRJP6 lacked any significant gene set enrichment, while MRJP8 and MRJP9 modulated similar sets. Notably, the neuronal-specific overexpression of MRJP1, MRJP2, MRJP3, and MRJP5 in Drosophila showed activated cell proliferation-related pathways and increased body size, highlighting their functional diversity and context-dependent effects. These findings expand our understanding of the functional roles of MRJPs and provide a foundation for further exploring their biological significance in honeybees and beyond.

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