Journal of Zhejiang University

Journal of Zhejiang University SCIENCE B

Accepted manuscript available online (unedited version)

A novel A-to-G mutation in circBDP1 alters adipocyte proliferation and differentiation and affects bovine carcass traits

Author(s): Enhui JIANG, Chiyuan ZHANG, Zhuoyuan HE, Yuli ZHANG, Yuta YANG, Chuanying PAN, Fugui JIANG, Enliang SONG, Sihuan ZHANG, Xianyong LAN
Affiliation(s): College of Animal Science and Technology, Northwest A&F University, Yangling 712100, China; more
Corresponding email(s): sihuanzhang1990@163.com, lanxianyong79@126.com
Key Words: Cattle, CircBDP1, Genetic variation, Carcass traits, Adipocyte

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Enhui JIANG, Chiyuan ZHANG, Zhuoyuan HE, Yuli ZHANG, Yuta YANG, Chuanying PAN, Fugui JIANG, Enliang SONG, Sihuan ZHANG, Xianyong LAN. A novel A-to-G mutation in circBDP1 alters adipocyte proliferation and differentiation and affects bovine carcass traits[J]. Journal of Zhejiang University Science B,in press.Frontiers of Information Technology & Electronic Engineering,in press.https://doi.org/10.1631/jzus.B2500084

@article{title="A novel A-to-G mutation in circBDP1 alters adipocyte proliferation and differentiation and affects bovine carcass traits",
author="Enhui JIANG, Chiyuan ZHANG, Zhuoyuan HE, Yuli ZHANG, Yuta YANG, Chuanying PAN, Fugui JIANG, Enliang SONG, Sihuan ZHANG, Xianyong LAN",
journal="Journal of Zhejiang University Science B",
year="in press",
publisher="Zhejiang University Press & Springer",
doi="https://doi.org/10.1631/jzus.B2500084"
}

%0 Journal Article
%T A novel A-to-G mutation in circBDP1 alters adipocyte proliferation and differentiation and affects bovine carcass traits
%A Enhui JIANG
%A Chiyuan ZHANG
%A Zhuoyuan HE
%A Yuli ZHANG
%A Yuta YANG
%A Chuanying PAN
%A Fugui JIANG
%A Enliang SONG
%A Sihuan ZHANG
%A Xianyong LAN
%J Journal of Zhejiang University SCIENCE B
%P 918-922
%@ 1673-1581
%D in press
%I Zhejiang University Press & Springer
doi="https://doi.org/10.1631/jzus.B2500084"

TY - JOUR
T1 - A novel A-to-G mutation in circBDP1 alters adipocyte proliferation and differentiation and affects bovine carcass traits
A1 - Enhui JIANG
A1 - Chiyuan ZHANG
A1 - Zhuoyuan HE
A1 - Yuli ZHANG
A1 - Yuta YANG
A1 - Chuanying PAN
A1 - Fugui JIANG
A1 - Enliang SONG
A1 - Sihuan ZHANG
A1 - Xianyong LAN
J0 - Journal of Zhejiang University Science B
SP - 918
EP - 922
%@ 1673-1581
Y1 - in press
PB - Zhejiang University Press & Springer
ER -
doi="https://doi.org/10.1631/jzus.B2500084"

Abstract
Chinese Summary
Academic Network
Reviewer Comment

Abstract: Fat deposition in cattle, influenced by factors such as genetics, age, sex, and nutrition, plays a crucial role in determining growth rates, beef yield, and meat quality. Among these, genetics is the predominant factor (Kim et al., 2020; Xing et al., 2025). Research indicates a high heritability for visceral (36%) and subcutaneous (57%) fat (Fox et al., 2012). Gene mutations that affect adipocyte function are significant contributors to variations in fat deposition and meat quality (Mannen, 2011; Mwangi et al., 2019; Sun et al., 2023; Dementieva et al., 2024). Therefore, investigating genetic variations in fat-related genes is essential for enhancing cattle performance.

circBDP1中A-G的新突变可影响牛脂肪细胞的增殖分化及胴体性状

蒋恩惠^1,2, 张驰原¹, 何卓远¹, 张彧丽¹, 杨钰塔¹, 潘传英¹, 姜富贵³, 宋恩亮³, 张思欢^1,2, 蓝贤勇¹
¹西北农林科技大学动物科技学院，中国杨凌， 712100
²安徽农业大学动物科技学院，中国合肥， 230036
³山东省农业科学院畜牧兽医研究所，中国济南， 250100
摘要：脂肪沉积是影响牛生长发育及肉产量和品质的重要因素。前期发现环状RNA circBDP1可促进牛脂肪细胞的增殖与分化。本研究通过鉴定circBDP1基因（rs454813519）上的A>G突变，采用Sanger测序与Kompetitive Allele-Specific PCR（KASP）技术，在秦川牛及山东黑牛遗传资源群体（Shandong Black Cattle Genetic Resource, SDBCGR）中检测到circBDP1-SNP位点的三种基因型（AA、AG和GG）。关联分析结果表明，该circBDP1-SNP A>G变异与SDBCGR母牛胸肌、上脑、牛肋排等性状和公牛胴体重、秦川牛体长等性状相关。为揭示该突变的相关功能，本研究构建了携带circBDP1-SNP不同等位基因的circBDP1-G/A过表达载体，并转染至牛前体脂肪细胞中。EdU检测、流式细胞术及qRT-PCR等实验结果显示，与circBDP1-G相比，circBDP1-A可显著增强前脂肪细胞的增殖与分化。此外，据已发表的全球牛基因组数据推测，G等位基因可能源自印度瘤牛。综上，circBDP1基因上的circBDP1-SNP A>G突变影响前脂肪细胞增殖与分化，可作为肉牛分子辅助选择的DNA标记。

关键词组：牛；CircBDP1；遗传变异；胴体性状；脂肪细胞

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

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