Journal of Zhejiang University

Journal of Zhejiang University SCIENCE B

Accepted manuscript available online (unedited version)

Development and application of a novel TaqMan qPCR assay targeting the gN gene for genotyping pseudorabies virus

Author(s): Yulan JIN, Xuan JI, Weiren DONG, Yanming HUANG, Jiyong ZHOU, Jinyan GU
Affiliation(s): The Experimental Teaching Center, College of Animal Sciences, Zhejiang University, Hangzhou 310058, China; more
Corresponding email(s): gujinyan@zju.edu.cn
Key Words: Pseudorabies virus, Glycoprotein N, Taqman qPCR, Genotyping, Detection methods

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Yulan JIN, Xuan JI, Weiren DONG, Yanming HUANG, Jiyong ZHOU, Jinyan GU. Development and application of a novel TaqMan qPCR assay targeting the gN gene for genotyping pseudorabies virus[J]. Journal of Zhejiang University Science B,in press.Frontiers of Information Technology & Electronic Engineering,in press.https://doi.org/10.1631/jzus.B2500729

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author="Yulan JIN, Xuan JI, Weiren DONG, Yanming HUANG, Jiyong ZHOU, Jinyan GU",
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doi="https://doi.org/10.1631/jzus.B2500729"
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%A Yulan JIN
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%A Weiren DONG
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%A Jiyong ZHOU
%A Jinyan GU
%J Journal of Zhejiang University SCIENCE B
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doi="https://doi.org/10.1631/jzus.B2500729"

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A1 - Jiyong ZHOU
A1 - Jinyan GU
J0 - Journal of Zhejiang University Science B
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doi="https://doi.org/10.1631/jzus.B2500729"

Abstract
Chinese Summary
Academic Network
Reviewer Comment

Abstract: Pseudorabies virus (PRV), known as porcine herpesvirus type I, belongs to the α-herpesvirus subfamily of the herpesviridae family. Its virions are oval or round, with a genome of approximately 143 000 bp and a high guanine-cytosine (GC) content of 73% (Pomeranz et al., 2005). While swine are the natural host and the virus has caused significant economic loss to the pig industry, PRV can infect other mammals, causing acute infectious diseases characterized by neurological symptoms (He et al., 2019; Cheng et al., 2020). Young pigs are more susceptible to PRV and the infection in piglets is nearly 100% fatal, whereas adult pigs exhibit reproductive disorders such as abortion, stillbirths, and reduced fertility, ultimately decreasing farm productivity (Zuckermann, 2000). Due to its global impact, PRV is classified as a Class B infectious disease by the World Organization for Animal Health (WOAH).

基于gN基因的伪狂犬病病毒TaqMan qPCR分型方法的建立及应用

金玉兰^1,2, 季暄¹, 董伟仁^1,2, 黄彦明^1,2, 周继勇^1,2, 顾金燕^1,2
¹浙江大学动物科学学院实验教学中心，中国杭州， 310058
² 浙江大学动物医学中心农业农村部动物病毒学重点实验室，中国杭州， 310058
摘要：伪狂犬病是由伪狂犬病病毒（PRV）引起的一种严重危害全球养猪业的传染病，由于不同基因型毒株的致病性与流行特征存在差异，临床上需建立一种能够快速、准确区分PRV基因I型和基因II型的分型检测方法。本研究首次以gN基因为靶标，基于其保守区域鉴别稳定差异序列，成功建立了一种TaqMan三重实时荧光定量聚合酶链式反应（PCR）检测方法。该方法不仅可检测PRV感染，而且能有效区分其基因型。该检测方法具有特异性强、灵敏度高、重复性好及操作简便等优点。该检测体系仅需一对引物和三个探针即可完成分型鉴定，极大简化了反应体系，降低了引物错配及非特异性扩增的风险。对实验动物来源样本初步应用的结果显示，该方法能准确检测PRV的感染情况及其基因型。本研究为PRV的分子流行病学监测提供了实用、高效的技术工具，也为猪群中PRV的净化工作提供了有力支持。

关键词组：伪狂犬病病毒（PRV）；gN基因；TaqMan；聚合酶链式反应（PCR）；分型；检测方法

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

Reference

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