JZUS - Journal of Zhejiang University SCIENCE

Journal of Zhejiang University SCIENCE B

Accepted manuscript available online (unedited version)

DNA based one-pot TLAMP and PfAgo rapid assay biosensing platform for meat visualized authentication

Author(s): Sheng DING^1, Jing LI^1, Minglong YANG^1*, Yujia SONG¹, Xiangke NIU¹, Mei LI², Yao LUO²
Affiliation(s): ¹Clinical Medical College & Affiliated Hospital, Chengdu University, Chengdu 610106, China ²Department of Laboratory Medicine, Sichuan Medical Laboratory Clinical Medicine Research Center, West China Hospital, Sichuan University, Chengdu 610041, China
Corresponding email(s): Sheng DING, dingsheng@cdu.edu.cn, Jing LI, 3496997885@qq.com, Minglong YANG, 850812060@qq.com, Yujia SONG, 3206647584@qq.com, Xiangke NIU, niu19850519@163.com
Key Words: TLAMP; PfAgo; Meat authentication; One-pot assay; Point-of-care testing

Share this article to： More <<< Previous Paper \|Next Paper >>>

Sheng DING^1*, Jing LI^1*, Minglong YANG^1*, Yujia SONG¹, Xiangke NIU¹, Mei LI², Yao LUO². DNA based one-pot TLAMP and PfAgo rapid assay biosensing platform for meat visualized authentication[J]. Journal of Zhejiang University Science B,in press.Frontiers of Information Technology & Electronic Engineering,in press.https://doi.org/10.1631/jzus.B2500745

@article{title="DNA based one-pot TLAMP and PfAgo rapid assay biosensing platform for meat visualized authentication",
author="Sheng DING^1*, Jing LI^1*, Minglong YANG^1*, Yujia SONG¹, Xiangke NIU¹, Mei LI², Yao LUO²",
journal="Journal of Zhejiang University Science B",
year="in press",
publisher="Zhejiang University Press & Springer",
doi="https://doi.org/10.1631/jzus.B2500745"
}

%0 Journal Article
%T DNA based one-pot TLAMP and PfAgo rapid assay biosensing platform for meat visualized authentication
%A Sheng DING^1*
%A Jing LI^1*
%A Minglong YANG^1*
%A Yujia SONG¹
%A Xiangke NIU¹
%A Mei LI²
%A Yao LUO²
%J Journal of Zhejiang University SCIENCE B
%P
%@ 1673-1581
%D in press
%I Zhejiang University Press & Springer
doi="https://doi.org/10.1631/jzus.B2500745"

TY - JOUR
T1 - DNA based one-pot TLAMP and PfAgo rapid assay biosensing platform for meat visualized authentication
A1 - Sheng DING^1*
A1 - Jing LI^1*
A1 - Minglong YANG^1*
A1 - Yujia SONG¹
A1 - Xiangke NIU¹
A1 - Mei LI²
A1 - Yao LUO²
J0 - Journal of Zhejiang University Science B
SP -
EP -
%@ 1673-1581
Y1 - in press
PB - Zhejiang University Press & Springer
ER -
doi="https://doi.org/10.1631/jzus.B2500745"

Abstract
Chinese Summary
Academic Network
Reviewer Comment

Abstract: Meat adulteration is a significant global food safety challenge, creating a pressing need for rapid and on-site detection technologies. Herein, we present an intelligent one-pot biosensing platform termed OTPA (One-pot TLAMP-PfAgo Assay) that integrates the rapid, isothermal amplification of turn-back loop primer-accelerated LAMP (TLAMP) with the sequence-specific detection of Pyrococcus furiosus Argonaute (PfAgo). This system features a clever heat-activatable design using microcrystalline wax to spatially separate reactions within a single tube, enabling contamination-free and streamlined operation. The OTPA assay achieves sensitive and specific detection, with limits of detection as low as 3×10^-4 ng/µL for pork DNA and 2×10^-4 ng/µL for beef DNA within 30 minutes. It successfully enables duplex target identification and has been validated with commercial meat products, showing perfect concordance with standard PCR-based qualitative detection. Notably, the result can be directly visualized under blue light, underscoring the substantial potential of this cost-effective and simple platform for point-of-care testing (POCT) and intelligent biosensing in food safety surveillance.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

Reference

Open peer comments: Debate/Discuss/Question/Opinion

<1>

- Go to

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

Reference