Journal of Zhejiang University

Journal of Zhejiang University SCIENCE B

Accepted manuscript available online (unedited version)

Cang-Fu-Dao-Tan Formula exerts positive multigenerational effects on the offspring of polyendocrine metabolic ovarian syndrome mouse model through alleviating uterine inflammation

Author(s): Weihuan HU1, Fangfang WANG1, 2, 3, 4, 5, Meiping LI1, 6, Ningning XIE1, Shaoyan YE7, Siwen ZHANG7, Wenshan ZENG1, Bizhen WANG7, Lu ZHAO8, Fan QU1, 2, 3, 4, Jue ZHOU7
Affiliation(s): 1Women’s Hospital, School of Medicine, Zhejiang University, Hangzhou 310006, China; more
Corresponding email(s): juezhou@zjgsu.edu.cn, syqufan@zju.edu.cn
Key Words: Polyendocrine metabolic ovarian syndrome, Cang-Fu-Dao-Tan Formula, Multigenerational effects, Uterine inflammation.

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Weihuan HU1, Fangfang WANG1,2,3,4,5, Meiping LI1,6, Ningning XIE1, Shaoyan YE7, Siwen ZHANG7, Wenshan ZENG1, Bizhen WANG7, Lu ZHAO8, Fan QU1,2,3,4, Jue ZHOU7. Cang-Fu-Dao-Tan Formula exerts positive multigenerational effects on the offspring of polyendocrine metabolic ovarian syndrome mouse model through alleviating uterine inflammation[J]. Journal of Zhejiang University Science B,in press.Frontiers of Information Technology & Electronic Engineering,in press.https://doi.org/10.1631/jzus.B2600515

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author="Weihuan HU1, Fangfang WANG1,2,3,4,5, Meiping LI1,6, Ningning XIE1, Shaoyan YE7, Siwen ZHANG7, Wenshan ZENG1, Bizhen WANG7, Lu ZHAO8, Fan QU1,2,3,4, Jue ZHOU7",
journal="Journal of Zhejiang University Science B",
year="in press",
publisher="Zhejiang University Press & Springer",
doi="https://doi.org/10.1631/jzus.B2600515"
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%A Fangfang WANG1
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%A Ningning XIE1
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%A Bizhen WANG7
%A Lu ZHAO8
%A Fan QU1
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%A Jue ZHOU7
%J Journal of Zhejiang University SCIENCE B
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doi="https://doi.org/10.1631/jzus.B2600515"

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T1 - Cang-Fu-Dao-Tan Formula exerts positive multigenerational effects on the offspring of polyendocrine metabolic ovarian syndrome mouse model through alleviating uterine inflammation
A1 - Weihuan HU1
A1 - Fangfang WANG1
A1 - 2
A1 - 3
A1 - 4
A1 - 5
A1 - Meiping LI1
A1 - 6
A1 - Ningning XIE1
A1 - Shaoyan YE7
A1 - Siwen ZHANG7
A1 - Wenshan ZENG1
A1 - Bizhen WANG7
A1 - Lu ZHAO8
A1 - Fan QU1
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A1 - Jue ZHOU7
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PB - Zhejiang University Press & Springer
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doi="https://doi.org/10.1631/jzus.B2600515"

Abstract
Chinese Summary
Academic Network
Reviewer Comment

Abstract: Background: Polyendocrine metabolic ovarian syndrome (PMOS), formerly known as polycystic ovary syndrome, is the most prevalent endocrine disorder among women of reproductive age. Cang-Fu-Dao-Tan formula (CFDTF), a traditional formula, has been reported to exert endocrine-metabolic regulatory effects in the treatment of PMOS. However, the effects and mechanisms by which CFDTF interrupts multigenerational PMOS-related features remain unknown. This study aimed to determine whether preconceptional CFDTF administration alleviates pathological phenotypes in the offspring of mothers with PMOS and to identify the potential underlying mechanisms. Methods: Prenatal anti-Mullerian hormone (PAMH)-induced PMOS mouse models (F0) were established, followed by preconceptional administration. Reproductive and glucolipid metabolic phenotypes were assessed in F1 and F2 offspring, and uterine transcriptomic profiling (RNA-seq) was performed in F0, F1, and F2 female offspring to investigate the potential mechanisms underlying CFDTF’s effects. Results: Preconceptional CFDTF administration significantly ameliorated reproductive abnormalities across generations. In F0-F2 females, it maintained estrous cyclicity, balanced testosterone levels, and reduced uterine inflammatory infiltration; in F1 males, it reduced abnormal seminiferous tubules. It also significantly improved glucolipid metabolism by lowering fasting glucose, total cholesterol, and hepatic lipid accumulation in F1 and F2 male and female offspring. RNA-seq analysis revealed multigenerational effects of preconceptional CFDTF administration on PMOS-associated uterine inflammatory signaling and immune microenvironment remodeling, which operate through a conserved cytokine network functionally linked to the interferon (IFN) inflammatory axis. Conclusions: Preconceptional CFDTF administration exerts positive multigenerational reproductive and glucolipid metabolic effects in a PMOS mouse model, through the alleviation of uterine inflammation.

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On-line Access: 2026-10-26

Received: 2026-07-13

Revision Accepted: 2026-10-14

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Citations: Bibtex RefMan EndNote GB/T7714

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