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On-line Access: 2024-08-27
Received: 2023-10-17
Revision Accepted: 2024-05-08
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Ca(V)3.3-Mediated Endochondral Ossification in a Threedimensional Bioprinted GelMA hydrogel
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Zhi Wang, Xin Wang, Yang Huang, Junjun Yang, Zu Wan, Zhenlan Fu, Xiaoyuan Gong, Guangxing Chen, Liu Yang. Ca(V)3.3-Mediated Endochondral Ossification in a Threedimensional Bioprinted GelMA hydrogel[J]. Journal of Zhejiang University Science , , (): .
@article{title="Ca(V)3.3-Mediated Endochondral Ossification in a Threedimensional Bioprinted GelMA hydrogel",
author="Zhi Wang, Xin Wang, Yang Huang, Junjun Yang, Zu Wan, Zhenlan Fu, Xiaoyuan Gong, Guangxing Chen, Liu Yang",
journal="Journal of Zhejiang University Science ",
volume="",
number="",
pages="",
year="",
publisher="Zhejiang University Press & Springer",
doi="10.1007/s42242-024-00287-1"
}
%0 Journal Article
%T Ca(V)3.3-Mediated Endochondral Ossification in a Threedimensional Bioprinted GelMA hydrogel
%A Zhi Wang
%A Xin Wang
%A Yang Huang
%A Junjun Yang
%A Zu Wan
%A Zhenlan Fu
%A Xiaoyuan Gong
%A Guangxing Chen
%A Liu Yang
%J Journal of Zhejiang University SCIENCE
%V
%N
%P
%@ 1869-1951
%D
%I Zhejiang University Press & Springer
%DOI 10.1007/s42242-024-00287-1
TY - JOUR
T1 - Ca(V)3.3-Mediated Endochondral Ossification in a Threedimensional Bioprinted GelMA hydrogel
A1 - Zhi Wang
A1 - Xin Wang
A1 - Yang Huang
A1 - Junjun Yang
A1 - Zu Wan
A1 - Zhenlan Fu
A1 - Xiaoyuan Gong
A1 - Guangxing Chen
A1 - Liu Yang
J0 - Journal of Zhejiang University Science
VL -
IS -
SP -
EP -
%@ 1869-1951
Y1 -
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1007/s42242-024-00287-1
Abstract: The growth plate (GP) is a crucial tissue involved in skeleton development via
endochondral ossification (EO). The bone organoid is a potential research model
capable of simulating the physiological function, spatial structure, and intercellular communication of native GPs. However, mimicking the EO process remains a key
challenge for bone organoid research. To simulate this orderly mineralization process,
we designed an in vitro shca(V)3.3 ATDC5-loaded gelatin methacryloyl (GelMA)
hydrogel model and evaluated its bioprintability for future organoid construction. In
this paper, we report the first demonstration that the T-type voltage-dependent
calcium channel (t-VDCC) subtype ca(V)3.3 is dominantly expressed in
chondrocytes and is negatively correlated with the hypertrophic differentiation of
chondrocytes during the EO process. Furthermore, ca(V)3.3 knockdown
chondrocytes loaded with the GelMA hydrogel successfully captured the EO process
and provide a bioink capable of constructing layered and orderly mineralized GP
organoids in the future. The results of this study could therefore provide a potential
target for regulating the EO process and a novel strategy for simulating it in bone
organoids.
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