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Journal of Zhejiang University SCIENCE A 2004 Vol.5 No.10 P.1255-1261

http://doi.org/10.1631/jzus.2004.1255


Lymphotactin enhances the in-vitro immune efficacy of dendritoma formed by dendritic cells and mouse hepatocellular carcinoma cells


Author(s):  ZHANG Hao, JIANG Guo-ping, ZHENG Shu-sen, WU Li-hua, ZHU Feng, YANG Zhen-lin

Affiliation(s):  First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310003, China

Corresponding email(s):   haozh@zju.edu.cn

Key Words:  Lymphotactin, Dendritic cell, Cell fusion, Hepatocellular carcinoma


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ZHANG Hao, JIANG Guo-ping, ZHENG Shu-sen, WU Li-hua, ZHU Feng, YANG Zhen-lin. Lymphotactin enhances the in-vitro immune efficacy of dendritoma formed by dendritic cells and mouse hepatocellular carcinoma cells[J]. Journal of Zhejiang University Science A, 2004, 5(10): 1255-1261.

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author="ZHANG Hao, JIANG Guo-ping, ZHENG Shu-sen, WU Li-hua, ZHU Feng, YANG Zhen-lin",
journal="Journal of Zhejiang University Science A",
volume="5",
number="10",
pages="1255-1261",
year="2004",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.2004.1255"
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%T Lymphotactin enhances the in-vitro immune efficacy of dendritoma formed by dendritic cells and mouse hepatocellular carcinoma cells
%A ZHANG Hao
%A JIANG Guo-ping
%A ZHENG Shu-sen
%A WU Li-hua
%A ZHU Feng
%A YANG Zhen-lin
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%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.2004.1255

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T1 - Lymphotactin enhances the in-vitro immune efficacy of dendritoma formed by dendritic cells and mouse hepatocellular carcinoma cells
A1 - ZHANG Hao
A1 - JIANG Guo-ping
A1 - ZHENG Shu-sen
A1 - WU Li-hua
A1 - ZHU Feng
A1 - YANG Zhen-lin
J0 - Journal of Zhejiang University Science A
VL - 5
IS - 10
SP - 1255
EP - 1261
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Y1 - 2004
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.2004.1255


Abstract: 
Objective: To investigate the in-vitro antitumor immune responses of dendritoma formed by mouse hepatocellular carcinoma (HCC) cells and lymphotactin (Lptn) gene modified dendritic cells (DCs). Method: DCs prepared from mouse bone marrow were genetically modified by lymphotactin adenovirus, and fused with H22 cells by polyethylene glycol (PEG). RT-PCR and ELISA were employed to identify lymphotactin expression at mRNA and protein level. Cell phenotypes and fusion efficiency was detected by FACS. The stimulatory effect of DC on T cells was detected by mixed lymphocyte reaction. The cytotoxicity activity against H22 cells was assayed by LDH method. Results: lymphotactin could be efficiently expressed by DCLptn/H22 hybridoma. DCLptn/H22 cells could induce potent T cell proliferation effect and generate strong cytotoxic T lymphocyte (CTL) reaction against allogenic H22 cells. Conclusion: lymphotactin genetic modification could enhance the in vitro immune activity of the dendritoma.

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Reference

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