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On-line Access: 2025-06-27
Received: 2024-09-02
Revision Accepted: 2025-01-12
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Integrating three-dimensional printing and bioprinting technologies to develop a stretchable in vitro model of the human airway
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Junned Chan,Julian Gonzalez Rubio,Oscar O´Dwyer Lancaster-Jones,Yashasvi Verma,Charlotte Büchter,Stefan Jockenhoevel,Laura De Laporte,Mirko Trilling,Anja Lena Thiebes,Daniela Duarte Campos. Integrating three-dimensional printing and bioprinting technologies to develop a stretchable in vitro model of the human airway[J]. Journal of Zhejiang University Science D, 2025, 8(4): 595–608.
@article{title="Integrating three-dimensional printing and bioprinting technologies to
develop a stretchable in vitro model of the human airway",
author="Junned Chan,Julian Gonzalez Rubio,Oscar O´Dwyer Lancaster-Jones,Yashasvi Verma,Charlotte Büchter,Stefan Jockenhoevel,Laura De Laporte,Mirko Trilling,Anja Lena Thiebes,Daniela Duarte Campos",
journal="Journal of Zhejiang University Science D",
volume="8",
number="4",
pages="595–608",
year="2025",
publisher="Zhejiang University Press & Springer",
doi="10.1631/bdm.2400351"
}
%0 Journal Article
%T Integrating three-dimensional printing and bioprinting technologies to
develop a stretchable in vitro model of the human airway
%A Junned Chan
%A Julian Gonzalez Rubio
%A Oscar O´Dwyer Lancaster-Jones
%A Yashasvi Verma
%A Charlotte Büchter
%A Stefan Jockenhoevel
%A Laura De Laporte
%A Mirko Trilling
%A Anja Lena Thiebes
%A Daniela Duarte Campos
%J Journal of Zhejiang University SCIENCE D
%V 8
%N 4
%P 595–608
%@ 1869-1951
%D 2025
%I Zhejiang University Press & Springer
%DOI 10.1631/bdm.2400351
TY - JOUR
T1 - Integrating three-dimensional printing and bioprinting technologies to
develop a stretchable in vitro model of the human airway
A1 - Junned Chan
A1 - Julian Gonzalez Rubio
A1 - Oscar O´Dwyer Lancaster-Jones
A1 - Yashasvi Verma
A1 - Charlotte Büchter
A1 - Stefan Jockenhoevel
A1 - Laura De Laporte
A1 - Mirko Trilling
A1 - Anja Lena Thiebes
A1 - Daniela Duarte Campos
J0 - Journal of Zhejiang University Science D
VL - 8
IS - 4
SP - 595–608
EP -
%@ 1869-1951
Y1 - 2025
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/bdm.2400351
Abstract: The global demand for in vitro respiratory airway models has surged due to the coronavirus disease 2019 (COVID-19) pan‐
demic. Current state-of-the-art models use polymer membranes to separate epithelial cells from other cell types, creating a
nonphysiological barrier. In this study, we applied three-dimensional (3D) printing and bioprinting to develop an in vitro
model where endothelial and epithelial cells were in direct contact, mimicking their natural arrangement. This proof-ofconcept model includes a culture chamber, with an endothelial bioink printed and perfused through an epithelial channel.
In silico simulations of the air velocity within the channel revealed shear stress values ranging from 0.13 to 0.39 Pa, aligning
with the desired in vivo shear stress observed in the bronchi regions (0.1–0.4 Pa). Biomechanical movements during resting
breathing were mimicked by incorporating a textile mesh positioned away from the cell–cell interface. The epithelial channel
demonstrated a capacity for compression and expansion of up to −14.7% and +6.4%, respectively. Microscopic images
showed that the epithelial cells formed a uniform monolayer within the lumen of the channel close to the bioprinted endothe‐
lial cells. Our novel model offers a valuable tool for future research into respiratory diseases and potential treatments under
conditions closely mimicking those in the lung.
Open peer comments: Debate/Discuss/Question/Opinion
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