CLC number:
On-line Access: 2024-03-26
Received: 2023-07-26
Revision Accepted: 2023-11-20
Crosschecked: 0000-00-00
Cited: 0
Clicked: 505
Xiaoyuan Wang, Zixian Liu, Qianqian Duan, Boye Zhang, Yanyan Cao, Zhizhong Shen, Meng Li, Yanfeng Xi, Jianming Wang & Shengbo Sang. 3D bioprinting of in vitro porous hepatoma models: establishment, evaluation, and anticancer drug testing[J]. Journal of Zhejiang University Science D, 2024, 7(2): 137-152.
@article{title="3D bioprinting of in vitro porous hepatoma models: establishment,
evaluation, and anticancer drug testing",
author="Xiaoyuan Wang, Zixian Liu, Qianqian Duan, Boye Zhang, Yanyan Cao, Zhizhong Shen, Meng Li, Yanfeng Xi, Jianming Wang & Shengbo Sang",
journal="Journal of Zhejiang University Science D",
volume="7",
number="2",
pages="137-152",
year="2024",
publisher="Zhejiang University Press & Springer",
doi="10.1007/s42242-023-00263-1"
}
%0 Journal Article
%T 3D bioprinting of in vitro porous hepatoma models: establishment,
evaluation, and anticancer drug testing
%A Xiaoyuan Wang
%A Zixian Liu
%A Qianqian Duan
%A Boye Zhang
%A Yanyan Cao
%A Zhizhong Shen
%A Meng Li
%A Yanfeng Xi
%A Jianming Wang & Shengbo Sang
%J Journal of Zhejiang University SCIENCE D
%V 7
%N 2
%P 137-152
%@ 1869-1951
%D 2024
%I Zhejiang University Press & Springer
%DOI 10.1007/s42242-023-00263-1
TY - JOUR
T1 - 3D bioprinting of in vitro porous hepatoma models: establishment,
evaluation, and anticancer drug testing
A1 - Xiaoyuan Wang
A1 - Zixian Liu
A1 - Qianqian Duan
A1 - Boye Zhang
A1 - Yanyan Cao
A1 - Zhizhong Shen
A1 - Meng Li
A1 - Yanfeng Xi
A1 - Jianming Wang & Shengbo Sang
J0 - Journal of Zhejiang University Science D
VL - 7
IS - 2
SP - 137
EP - 152
%@ 1869-1951
Y1 - 2024
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1007/s42242-023-00263-1
Abstract: Traditional tumor models do not tend to accurately simulate tumor growth in vitro or enable personalized treatment and
are particularly unable to discover more beneficial targeted drugs. To address this, this study describes the use of threedimensional (3D) bioprinting technology to construct a 3D model with human hepatocarcinoma SMMC-7721 cells (3DP-
7721) by combining gelatin methacrylate (GelMA) and poly(ethylene oxide) (PEO) as two immiscible aqueous phases to
form a bioink and innovatively applying fluorescent carbon quantum dots for long-term tracking of cells. The GelMA (10%,
mass fraction) and PEO (1.6%, mass fraction) hydrogel with 3:1 volume ratio offered distinct pore-forming characteristics,
satisfactory mechanical properties, and biocompatibility for the creation of the 3DP-7721 model. Immunofluorescence analysis
and quantitative real-time fluorescence polymerase chain reaction (PCR) were used to evaluate the biological properties of the
model. Compared with the two-dimensional culture cell model (2D-7721) and the 3D mixed culture cell model (3DM-7721),
3DP-7721 significantly improved the proliferation of cells and expression of tumor-related proteins and genes. Moreover,
we evaluated the differences between the three culture models and the effectiveness of antitumor drugs in the three models
and discovered that the efficacy of antitumor drugs varied because of significant differences in resistance proteins and genes
between the three models. In addition, the comparison of tumor formation in the three models found that the cells cultured by
the 3DP-7721 model had strong tumorigenicity in nude mice. Immunohistochemical evaluation of the levels of biochemical
indicators related to the formation of solid tumors showed that the 3DP-7721 model group exhibited pathological characteristics
of malignant tumors, the generated solid tumors were similar to actual tumors, and the deterioration was higher. This research
therefore acts as a foundation for the application of 3DP-7721 models in drug development research.
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