CLC number: S432.41
On-line Access:
Received: 2001-07-18
Revision Accepted: 2001-11-29
Crosschecked: 0000-00-00
Cited: 27
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ZHANG Heng-mu, CHEN Jian-ping, XUE Qing-zhong, LEI Juan-li. cDNA cloning and sequence analysis of genome segments S8 from rice black-streaked dwarf virus[J]. Journal of Zhejiang University Science A, 2002, 3(2): 216-222.
@article{title="cDNA cloning and sequence analysis of genome segments S8 from rice black-streaked dwarf virus",
author="ZHANG Heng-mu, CHEN Jian-ping, XUE Qing-zhong, LEI Juan-li",
journal="Journal of Zhejiang University Science A",
volume="3",
number="2",
pages="216-222",
year="2002",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.2002.0216"
}
%0 Journal Article
%T cDNA cloning and sequence analysis of genome segments S8 from rice black-streaked dwarf virus
%A ZHANG Heng-mu
%A CHEN Jian-ping
%A XUE Qing-zhong
%A LEI Juan-li
%J Journal of Zhejiang University SCIENCE A
%V 3
%N 2
%P 216-222
%@ 1869-1951
%D 2002
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.2002.0216
TY - JOUR
T1 - cDNA cloning and sequence analysis of genome segments S8 from rice black-streaked dwarf virus
A1 - ZHANG Heng-mu
A1 - CHEN Jian-ping
A1 - XUE Qing-zhong
A1 - LEI Juan-li
J0 - Journal of Zhejiang University Science A
VL - 3
IS - 2
SP - 216
EP - 222
%@ 1869-1951
Y1 - 2002
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.2002.0216
Abstract: Genome segments S8 of two Chinese isolates of rice black-streaked dwarf virus (RBSDV), one from Zhejiang Province and another from Hebei Province, were amplified by RT-PCR and sequenced. Both segments consisted of 1936 nts in full length (EMBL accession numbers were AJ297431 and AJ297432, respectively) and contained only one big open reading frame which encoded a polypeptide with molecular weight of 68kD. The two Chinese isolates shared 94.0% and 96.5% identity at nucleotide and amino acid level, respectively. They shared 94.5-94.9% and 92.5-92.9% homology with S8 of RBSDV Japanese isolate at nucleotide and amino acid level, respectively; shared 85.1-87.6% and 91.7-91.9% homology with S7 of Italian MRDV (maize rough dwarf virus).
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