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CLC number: Q933
On-line Access: 2024-08-27
Received: 2023-10-17
Revision Accepted: 2024-05-08
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Mutation breeding of high 4-androstene-3,17-dione-producing Mycobacterium neoaurum ZADF-4 by atmospheric and room temperature plasma treatment
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Chao Liu, Xian Zhang, Zhi-ming Rao, Ming-long Shao, Le-le Zhang, Dan Wu, Zheng-hong Xu, Hui Li. Mutation breeding of high 4-androstene-3,17-dione-producing Mycobacterium neoaurum ZADF-4 by atmospheric and room temperature plasma treatment[J]. Journal of Zhejiang University Science B, 2015, 16(4): 286-295.
@article{title="Mutation breeding of high 4-androstene-3,17-dione-producing Mycobacterium neoaurum ZADF-4 by atmospheric and room temperature plasma treatment",
author="Chao Liu, Xian Zhang, Zhi-ming Rao, Ming-long Shao, Le-le Zhang, Dan Wu, Zheng-hong Xu, Hui Li",
journal="Journal of Zhejiang University Science B",
volume="16",
number="4",
pages="286-295",
year="2015",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1400274"
}
%0 Journal Article
%T Mutation breeding of high 4-androstene-3,17-dione-producing Mycobacterium neoaurum ZADF-4 by atmospheric and room temperature plasma treatment
%A Chao Liu
%A Xian Zhang
%A Zhi-ming Rao
%A Ming-long Shao
%A Le-le Zhang
%A Dan Wu
%A Zheng-hong Xu
%A Hui Li
%J Journal of Zhejiang University SCIENCE B
%V 16
%N 4
%P 286-295
%@ 1673-1581
%D 2015
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1400274
TY - JOUR
T1 - Mutation breeding of high 4-androstene-3,17-dione-producing Mycobacterium neoaurum ZADF-4 by atmospheric and room temperature plasma treatment
A1 - Chao Liu
A1 - Xian Zhang
A1 - Zhi-ming Rao
A1 - Ming-long Shao
A1 - Le-le Zhang
A1 - Dan Wu
A1 - Zheng-hong Xu
A1 - Hui Li
J0 - Journal of Zhejiang University Science B
VL - 16
IS - 4
SP - 286
EP - 295
%@ 1673-1581
Y1 - 2015
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1400274
Abstract: Steroid medication is used extensively in clinical applications and comprises a large and vital part of the pharmaceutical industry. However, the difficulty of separating 4-Androstene-3,17-dione (AD)%29&ck%5B%5D=abstract&ck%5B%5D=keyword'>17-dione (AD) from 1,4-Androstadiene-3,17-dione (ADD) restricts the application of the microbial transformation of phytosterols in the industry. A novel atmospheric and room temperature plasma (ARTP) treatment, which employs helium as the working gas, was used to generate Mycobacterium neoaurum mutants producing large amounts of AD. After treatment of cultures with ARTP, four mutants were selected using a novel screening method with a color assay. Among the mutants, M. neoaurum ZADF-4 was considered the best candidate for industrial application. When the fermentation medium contained 15 g/L phytosterols and was cultivated on a rotary shaker at 160 r/min at 30 °C for 7 d, (6.28 1;0.11) g/L of AD and (0.82 1;0.05) g/L of ADD were produced by the ZADF-4 mutant, compared with (4.83 1;0.13) g/L of AD and (2.34 1;0.06) g/L of ADD by the original strain, M. neoaurum ZAD. Compared with ZAD, the molar yield of AD increased from 48.3% to 60.3% in the ZADF-4 mutant. This result indicates that ZADF-4 may have potential for industrial production of AD.
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