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On-line Access: 2024-08-27
Received: 2023-10-17
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Generation of insect-resistant and glyphosate-tolerant rice by introduction of a T-DNA containing two Bt insecticidal genes and an EPSPS gene
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Qi-chao Zhao, Ming-hong Liu, Xian-wen Zhang, Chao-yang Lin, Qing Zhang, Zhi-cheng Shen. Generation of insect-resistant and glyphosate-tolerant rice by introduction of a T-DNA containing two Bt insecticidal genes and an EPSPS gene[J]. Journal of Zhejiang University Science B, 2015, 16(10): 824-831.
@article{title="Generation of insect-resistant and glyphosate-tolerant rice by introduction of a T-DNA containing two Bt insecticidal genes and an EPSPS gene",
author="Qi-chao Zhao, Ming-hong Liu, Xian-wen Zhang, Chao-yang Lin, Qing Zhang, Zhi-cheng Shen",
journal="Journal of Zhejiang University Science B",
volume="16",
number="10",
pages="824-831",
year="2015",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1500056"
}
%0 Journal Article
%T Generation of insect-resistant and glyphosate-tolerant rice by introduction of a T-DNA containing two Bt insecticidal genes and an EPSPS gene
%A Qi-chao Zhao
%A Ming-hong Liu
%A Xian-wen Zhang
%A Chao-yang Lin
%A Qing Zhang
%A Zhi-cheng Shen
%J Journal of Zhejiang University SCIENCE B
%V 16
%N 10
%P 824-831
%@ 1673-1581
%D 2015
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1500056
TY - JOUR
T1 - Generation of insect-resistant and glyphosate-tolerant rice by introduction of a T-DNA containing two Bt insecticidal genes and an EPSPS gene
A1 - Qi-chao Zhao
A1 - Ming-hong Liu
A1 - Xian-wen Zhang
A1 - Chao-yang Lin
A1 - Qing Zhang
A1 - Zhi-cheng Shen
J0 - Journal of Zhejiang University Science B
VL - 16
IS - 10
SP - 824
EP - 831
%@ 1673-1581
Y1 - 2015
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1500056
Abstract: Insect resistance and glyphosate tolerance have been two of the most important traits in the genetic improvement of various crops. In this study, two Bacillus thuringiensis (Bt) insecticidal genes, Cry1Ac and Cry1Ig, and a modified glyphosate-tolerant 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) gene (G10) were combined into a single transferred DNA (T-DNA) fragment and introduced into rice by Agrobacterium-mediated transformation. A transgenic line with single-copy T-DNA insertion named GAI-14 was found to be highly resistant to striped stem borer and rice leaf roller, and tolerant to glyphosate. Analysis of T-DNA border sequence suggested that the transgenes were inserted at the chromosome 3 and appeared to have not interrupted any known or putative genes. A field trial observed no significant difference in the basic agronomic traits between GAI-14 and the recipient rice.
[1]Alvi, A.H., Sayyed, A.H., Naeem, M., et al., 2012. Field evolved resistance in Helicoverpa armigera (Lepidoptera: Noctuidae) to Bacillus thuringiensis toxin Cry1Ac in Pakistan. PLoS ONE, 7(10):e47309.
[2]Cao, J., Zhao, J.Z., Tang, J.D., et al., 2002. Broccoli plants with pyramided cry1Ac and cry1C Bt genes control diamondback moths resistant to Cry1A and Cry1C proteins. Theor. Appl. Genet., 105(2-3):258-264.
[3]Dhurua, S., Gujar, G.T., 2011. Field-evolved resistance to Bt toxin Cry1Ac in the pink bollworm, Pectinophora gossypiella (Saunders) (Lepidoptera: Gelechiidae), from India. Pest Manag. Sci., 67(8):898-903.
[4]Ferré, J., Real, M.D., van Rie, J., et al., 1991. Resistance to the Bacillus thuringiensis bioinsecticide in a field population of Plutella xylostella is due to a change in a midgut membrane receptor. PNAS, 88(12):5119-5123.
[5]Gassmann, A.J., Petzold-Maxwell, J.L., Keweshan, R.S., et al., 2011. Field-evolved resistance to Bt maize by western corn rootworm. PLoS ONE, 6(7):e22629.
[6]Halpin, C., 2005. Gene stacking in transgenic plants—the challenge for 21st century plant biotechnology. Plant Biotechnol. J., 3(2):141-155.
[7]Hiei, Y., Ohta, S., Komari, T., et al., 1994. Efficient transformation of rice (Oryza sativa L.) mediated by Agrobacterium and sequence analysis of the boundaries of the T-DNA. Plant J., 6(2):271-282.
[8]James, C., 2013. Global Status of Commercialized Biotech/GM Crops: 2013. ISAAA Brief No. 46. International Service for the Acquisition of Agri-biotech Applications (ISAAA), Ithaca, NY, USA, p.315.
[9]Kruger, M., Rensburg, J.R.J.V., Berg, J.V.D., 2011. Resistance to Bt maize in Busseola fusca (Lepidoptera: Noctuidae) from Vaalharts, South Africa. Environ. Entomol., 40(2):477-483.
[10]Li, J., Yu, H., Zhang, F.Z., et al., 2013. A built-in strategy to mitigate transgene spreading from genetically modified corn. PLoS ONE, 8(12):e81645
[11]Liu, Y.G., Mitsukawa, N., Oosumi, T., et al., 1995. Efficient isolation and mapping of Arabidopsis thaliana T-DNA insert junctions by thermal asymmetric interlaced PCR. Plant J., 8(3):457-463.
[12]Ruiz de Escudero, I., Estela, A., Porcar, M., et al., 2006. Molecular and insecticidal characterization of a Cry1I protein toxic to insects of the families Noctuidae, Tortricidae, Plutellidae, and Chrysomelidae. Appl. Environ. Microbiol., 72(7):4796-4804.
[13]Sambrock, J., Russel, D.W., 2001. Molecular Cloning: A Laboratory Manual, 3rd Ed. Cold Spring Harbor Laboratory Press, New York.
[14]Storer, N.P., Babcock, J.M., Schlenz, M., et al., 2010. Discovery and characterization of field resistance to Bt maize: Spodoptera frugiperda (Lepidoptera: Noctuidae) in Puerto Rico. J. Econ. Entomol., 103(4):1031-1038.
[15]Wang, P., Zhao, J.Z., Rodrigo-Simon, A., et al., 2006. Mechanism of resistance to Bacillus thuringiensis toxin Cry1Ac in a greenhouse population of cabbage looper, Trichoplusia ni. Appl. Environ. Microbiol., 73(4):1199-1207.
[16]Yang, Y., Xu, H., Zheng, X., et al., 2012. Susceptibility and selectivity of Cnaphalocrocis medinalis (Lepidoptera: Pyralidae) to different Cry toxins. J. Econ. Entomol., 105(6):2122-2128.
[17]Zhang, H.N., Tian, W., Zhao, J., et al., 2012. Diverse genetic basis of field-evolved resistance to Bt cotton in cotton bollworm from China. PNAS, 109(26):10275-10280.
[18]Zhang, Q., Yu, H., Zhang, F.Z., 2013. Expression and purification of recombinant human serum albumin from selectively terminable transgenic rice. J. Zhejiang Univ.-Sci. B (Biomed. & Biotechnol.), 14(10):867-874.
[19]Zhao, J.Z., Cao, J., Li, Y.X., et al., 2003. Transgenic plants expressing two Bacillus thuringiensis toxins delay insect resistance evolution. Nat. Biotechnol., 21(12):1493-1497.
[20]Zhao, Q., Liu, M., Tan, M., et al., 2014. Expression of Cry1Ab and Cry2Ab by a polycistronic transgene with a self-cleavage peptide in rice. PLoS ONE, 9(10):e110006.
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