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Received: 2004-11-12

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Journal of Zhejiang University SCIENCE B 2005 Vol.6 No.7 P.664-667


Character of HBV (hepatitis B virus) polymerase gene rtM204V/I and rtL180M mutation in patients with lamivudine resistance

Author(s):  LI Min-wei, HOU Wei, WO Jian-er, LIU Ke-zhou

Affiliation(s):  Institute of Infectious Diseases, First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310003, China

Corresponding email(s):   limw@zju.edu.cn

Key Words:  Hepatitis B virus, Lamivudine, YMDD mutant, Sequence analysis

LI Min-wei, HOU Wei, WO Jian-er, LIU Ke-zhou. Character of HBV (hepatitis B virus) polymerase gene rtM204V/I and rtL180M mutation in patients with lamivudine resistance[J]. Journal of Zhejiang University Science B, 2005, 6(7): 664-667.

@article{title="Character of HBV (hepatitis B virus) polymerase gene rtM204V/I and rtL180M mutation in patients with lamivudine resistance",
author="LI Min-wei, HOU Wei, WO Jian-er, LIU Ke-zhou",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Character of HBV (hepatitis B virus) polymerase gene rtM204V/I and rtL180M mutation in patients with lamivudine resistance
%A LI Min-wei
%A HOU Wei
%A WO Jian-er
%A LIU Ke-zhou
%J Journal of Zhejiang University SCIENCE B
%V 6
%N 7
%P 664-667
%@ 1673-1581
%D 2005
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.2005.B0664

T1 - Character of HBV (hepatitis B virus) polymerase gene rtM204V/I and rtL180M mutation in patients with lamivudine resistance
A1 - LI Min-wei
A1 - HOU Wei
A1 - WO Jian-er
A1 - LIU Ke-zhou
J0 - Journal of Zhejiang University Science B
VL - 6
IS - 7
SP - 664
EP - 667
%@ 1673-1581
Y1 - 2005
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.2005.B0664

Objectives: To investigate the relationship between HBV (hepatitis B virus) polymerase gene 180 and 204 sites mutation and lamivudine resistance. Methods: One hundred forty-one patients with lamivudine resistance after lamivudine treatment and 60 chronic hepatitis B patients without lamivudine treatment were enrolled in this study. The serum HBV DNA mutation was analyzed by sequence detection via polymerase chain reaction (PCR). The sequences of the same patient were analyzed before and after lamivudine treatment. Results: One hundred and nine lamivudine resistance patients had HBV YMDD (tyrosine-methionine-aspartate-aspartate) mutation. Among them, 45 patients had rtL180M/M204V mutation (41.28%), 28 patients had rtL180M/M204I mutation (25.70%) and 36 patients had rtM204I mutation (33.02%). There were 6 patients with rtL180M mutation in 32 lamivudine resistance patients. Sixty chronic hepatitis patients without lamivudine treatment had no mutations. Conclusions: HBV mutations, which play an important role in lamivudine resistance usually locate at polymerase gene 204 site; 180 site mutation was also observed in these patients. Evaluation of the anti-virus therapy by surveillance of the two sites mutations is of importance.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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