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On-line Access: 2021-01-27
Received: 2020-08-17
Revision Accepted: 2020-09-01
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Facile 3D cell culture protocol based on photocurable hydrogels
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Mingjun Xie, Yating Zheng, Qing Gao, Yong He. Facile 3D cell culture protocol based on photocurable hydrogels[J]. Journal of Zhejiang University Science D, 2021, 4(1): 149-153.
@article{title="Facile 3D cell culture protocol based on photocurable hydrogels",
author="Mingjun Xie, Yating Zheng, Qing Gao, Yong He",
journal="Journal of Zhejiang University Science D",
volume="4",
number="1",
pages="149-153",
year="2021",
publisher="Zhejiang University Press & Springer",
doi="10.1007/s42242-020-00096-2"
}
%0 Journal Article
%T Facile 3D cell culture protocol based on photocurable hydrogels
%A Mingjun Xie
%A Yating Zheng
%A Qing Gao
%A Yong He
%J Journal of Zhejiang University SCIENCE D
%V 4
%N 1
%P 149-153
%@ 1869-1951
%D 2021
%I Zhejiang University Press & Springer
%DOI 10.1007/s42242-020-00096-2
TY - JOUR
T1 - Facile 3D cell culture protocol based on photocurable hydrogels
A1 - Mingjun Xie
A1 - Yating Zheng
A1 - Qing Gao
A1 - Yong He
J0 - Journal of Zhejiang University Science D
VL - 4
IS - 1
SP - 149
EP - 153
%@ 1869-1951
Y1 - 2021
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1007/s42242-020-00096-2
Abstract:
本Technical Note聚焦光固化生物3D打印之高精度水凝胶支架制造。投影式光固化生物打印(Projection-based 3D bioprinting, PBP)具有精度高、速度快、自支撑等优势，近年来在生物制造领域有越来越多PBP的成功应用案例。然而，完全理解PBP的原理需要光学、高分子、工程、生物学等综合学科的背景知识，初学者熟练掌握PBP的流程也需要经历长时间的试错与经验积累。降低PBP的入门使用门槛，可使更多研究者能从这项技术中受益。
本文给出了一个标准的水凝胶支架打印流程，我们将PBP生物支架打印分为“材料配制”、“支架打印”、“后处理”与“细胞接种”四个工序，给出了标准化的操作方法，整理了很多常见的问题、技巧及背后机制。通过这篇教程，希望能够帮助PBP初学者，降低非工程学科背景使用者在工艺测试上的时间和成本，将PBP作为得力工具加速其研究。
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