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On-line Access: 2022-08-26
Received: 2022-02-17
Revision Accepted: 2022-06-13
Crosschecked: 2022-08-26
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Generation of ring-shaped human iPSC-derived functional heart microtissues in a Möbius strip configuration
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Yan Xu, Jingqi Qi, Wenyan Zhou, Xing Liu, Longbo Zhang, Xudong Yao & Hongwei Wu. Generation of ring-shaped human iPSC-derived functional heart microtissues in a Möbius strip configuration[J]. Journal of Zhejiang University Science D, 2022, 5(4): 687-699.
@article{title="Generation of ring-shaped human iPSC-derived functional heart microtissues in a Möbius strip configuration",
author="Yan Xu, Jingqi Qi, Wenyan Zhou, Xing Liu, Longbo Zhang, Xudong Yao & Hongwei Wu",
journal="Journal of Zhejiang University Science D",
volume="5",
number="4",
pages="687-699",
year="2022",
publisher="Zhejiang University Press & Springer",
doi="10.1007/s42242-022-00204-4"
}
%0 Journal Article
%T Generation of ring-shaped human iPSC-derived functional heart microtissues in a Möbius strip configuration
%A Yan Xu
%A Jingqi Qi
%A Wenyan Zhou
%A Xing Liu
%A Longbo Zhang
%A Xudong Yao & Hongwei Wu
%J Journal of Zhejiang University SCIENCE D
%V 5
%N 4
%P 687-699
%@ 1869-1951
%D 2022
%I Zhejiang University Press & Springer
%DOI 10.1007/s42242-022-00204-4
TY - JOUR
T1 - Generation of ring-shaped human iPSC-derived functional heart microtissues in a Möbius strip configuration
A1 - Yan Xu
A1 - Jingqi Qi
A1 - Wenyan Zhou
A1 - Xing Liu
A1 - Longbo Zhang
A1 - Xudong Yao & Hongwei Wu
J0 - Journal of Zhejiang University Science D
VL - 5
IS - 4
SP - 687
EP - 699
%@ 1869-1951
Y1 - 2022
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1007/s42242-022-00204-4
Abstract: Although human-induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) have been used for disease modeling and drug discovery, clinically relevant three-dimensional (3D) functional myocardial microtissues are lacking. Here, we developed a novel ring-shaped cardiac microtissue comprised of chamber-specific tissues to achieve a geometrically non-orientable ventricular myocardial band, similar to a Möbius loop. The ring-shaped cardiac tissue was constructed of hiPSC-CMs and human cardiac fibroblasts (hCFs) through a facile cellular self-assembly approach. It exhibited basic anatomical structure, positive cardiac troponin T (cTnT) immunostaining, regular calcium transients, and cardiac-like mechanical strength. The cardiac rings can be self-assembled and scaled up into various sizes with outstanding stability, suggesting their potential for precise therapy, pathophysiological investigation, and large-scale drug screening.
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