Full Text:   <301>

CLC number: 

On-line Access: 2023-07-07

Received: 2022-09-12

Revision Accepted: 2023-01-31

Crosschecked: 0000-00-00

Cited: 0

Clicked: 310

Citations:  Bibtex RefMan EndNote GB/T7714

-   Go to

Article info.
Open peer comments

Bio-Design and Manufacturing  2023 Vol.6 No.5 P.586–608

http://doi.org/10.1007/s42242-023-00236-4


Advanced strategies in the application of gelatin-based bioink for extrusion bioprinting


Author(s):  Jirong Yang, Huimin He, Duo Li, Qian Zhang, Lizhi Xu & Changshun Ruan

Affiliation(s):  Research Center for Human Tissue and Organs Degeneration, Institute of Biomedicine and Biotechnology, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China; more

Corresponding email(s):   cs.ruan@siat.ac.cn

Key Words:  Gelatin-based bioink, Extrusion bioprinting, Tissue-like construct


Share this article to: More

Jirong Yang, Huimin He, Duo Li, Qian Zhang, Lizhi Xu & Changshun Ruan. Advanced strategies in the application of gelatin-based bioink for extrusion bioprinting[J]. Journal of Zhejiang University Science D, 2023, 6(5): 586–608.

@article{title="Advanced strategies in the application of gelatin-based bioink for extrusion bioprinting",
author="Jirong Yang, Huimin He, Duo Li, Qian Zhang, Lizhi Xu & Changshun Ruan",
journal="Journal of Zhejiang University Science D",
volume="6",
number="5",
pages="586–608",
year="2023",
publisher="Zhejiang University Press & Springer",
doi="10.1007/s42242-023-00236-4"
}

%0 Journal Article
%T Advanced strategies in the application of gelatin-based bioink for extrusion bioprinting
%A Jirong Yang
%A Huimin He
%A Duo Li
%A Qian Zhang
%A Lizhi Xu & Changshun Ruan
%J Journal of Zhejiang University SCIENCE D
%V 6
%N 5
%P 586–608
%@ 1869-1951
%D 2023
%I Zhejiang University Press & Springer
%DOI 10.1007/s42242-023-00236-4

TY - JOUR
T1 - Advanced strategies in the application of gelatin-based bioink for extrusion bioprinting
A1 - Jirong Yang
A1 - Huimin He
A1 - Duo Li
A1 - Qian Zhang
A1 - Lizhi Xu & Changshun Ruan
J0 - Journal of Zhejiang University Science D
VL - 6
IS - 5
SP - 586–608
EP -
%@ 1869-1951
Y1 - 2023
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1007/s42242-023-00236-4


Abstract: 
The significance of bioink suitability for the extrusion bioprinting of tissue-like constructs cannot be overemphasized. Gelatin, derived from the hydrolysis of collagen, not only can mimic the extracellular matrix to immensely support cell function, but also is suitable for extrusion under certain conditions. Thus, gelatin has been recognized as a promising bioink for extrusion bioprinting. However, the development of a gelatin-based bioink with satisfactory printability and bioactivity to fabricate complex tissue-like constructs with the desired physicochemical properties and biofunctions for a specific biomedical application is still in its infancy. Therefore, in this review, we aim to comprehensively summarize the state-of-the-art methods of gelatin-based bioink application for extrusion bioprinting. We firstly outline the properties and requirements of gelatin-based bioinks for extrusion bioprinting, highlighting the strategies to overcome their main limitations in terms of printability, structural stability and cell viability. Then, the challenges and prospects are further discussed regarding the development of ideal gelatin-based bioinks for extrusion bioprinting to create complex tissue-like constructs with preferable physicochemical properties and biofunctions.

中国科学院阮长顺等 | 推动明胶基生物墨水应用于挤出式生物打印的最新进展

本综述论文主要聚焦于明胶基生物墨水在克服可打印性、结构稳定性和细胞活性方面局限性的最新策略。合适的生物墨水在挤出式生物打印构建复杂组织中扮演着极其重要的角色。明胶是胶原蛋白的水解产物,不仅能模拟细胞外基质、支持细胞的功能,而且在一定条件下可以挤出成型,是公认地在挤出式生物打印中最具有前景的生物墨水之一。但明胶本身可打印性不高、形状保真度和稳定性较差,同时力学强度不足。因此,开发兼具良好打印性和生物活性的明胶基生物墨水以构建复杂的组织,并应用于特定的生物医学领域仍处于起步阶段。本文全面系统地总结了明胶基生物墨水在挤出式生物打印中提高可打印性、实现复杂组织构建的物理和化学方法和策略。首先,总体概述了用于挤出式生物打印明胶基生物墨水需要满足的性能和要求。其次,重点论述了明胶在克服可打印性、结构稳定性和细胞活性方面局限性的最新策略。在此基础上,进一步探讨采用明胶基生物墨水构建具有更好理化性能和生物功能的复杂组织未来发展所面临的挑战和前景,为开发理想的明胶基生物墨水提供参考和借鉴。

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

Open peer comments: Debate/Discuss/Question/Opinion

<1>

Please provide your name, email address and a comment





Journal of Zhejiang University-SCIENCE, 38 Zheda Road, Hangzhou 310027, China
Tel: +86-571-87952783; E-mail: cjzhang@zju.edu.cn
Copyright © 2000 - 2024 Journal of Zhejiang University-SCIENCE