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Bio-Design and Manufacturing  2023 Vol.6 No.2 P.136-149

http://doi.org/10.1007/s42242-022-00225-z


Functionalized alginate-based bioinks for microscale electrohydrodynamic bioprinting of living tissue constructs with improved cellular spreading and alignment


Author(s):  Zhennan Qiu, Hui Zhu, Yutao Wang, Ayiguli Kasimu, Dichen Li & Jiankang He

Affiliation(s):  State Key Laboratory for Manufacturing Systems Engineering, Xi’an Jiaotong University, Xi’an 710049, China; more

Corresponding email(s):   jiankanghe@mail.xjtu.edu.cn

Key Words:  Microscale electrohydrodynamic bioprinting, Alginate-based bioinks, Cell spreading, Cell alignment


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Zhennan Qiu, Hui Zhu, Yutao Wang, Ayiguli Kasimu, Dichen Li & Jiankang He. Functionalized alginate-based bioinks for microscale electrohydrodynamic bioprinting of living tissue constructs with improved cellular spreading and alignment[J]. Journal of Zhejiang University Science D, 2023, 6(2): 136-149.

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Abstract: 
Bioprinting has been widely investigated for tissue engineering and regenerative medicine applications. However, it is still difficult to reconstruct the complex native cell arrangement due to the limited printing resolution of conventional bioprinting techniques such as extrusion- and inkjet-based printing. Recently, an electrohydrodynamic (EHD) bioprinting strategy was reported for the precise deposition of well-organized cell-laden constructs with microscale filament size, whereas few studies have been devoted to developing bioinks that can be applied for EHD bioprinting and simultaneously support cell spreading. This study describes functionalized alginate-based bioinks for microscale EHD bioprinting using peptide grafting and fibrin incorporation, which leads to high cell viability (>90%) and cell spreading. The printed filaments can be further refined to as small as 30 μm by incorporating polyoxyethylene and remained stable over one week when exposed to an aqueous environment. By utilizing the presented alginate-based bioinks, layer-specific cell alignment along the printing struts could be observed inside the EHD-printed microscale filaments, which allows fabricating living constructs with cell-scale filament resolution for guided cellular orientation.

西安交通大学贺健康团队 | 功能化海藻酸钠墨水细胞静电打印微米精度活性结构改善细胞铺展与定向效果

本研究论文聚焦于海藻酸钠墨水的功能化改性与相关高精度细胞静电打印工艺研究。生物打印技术在组织工程和再生医疗中有巨大的应用前景,但传统生物打印技术由于精度的限制在精确复现自然组织中细胞复杂排列结构方面仍然面临巨大挑战。近年来,一种利用电流体动力学效应打印出微米级别水凝胶纤维的细胞静电打印技术被提出,其在保证细胞活性的前提下极大提高了打印分辨率。但适用于细胞静电打印技术同能支持细胞铺展的生物墨水体系有待进一步研究。本研究通过化学修饰RGD、混合牛纤维蛋白原及添加PEO,得到了适用于细胞静电打印工艺的功能化海藻酸钠墨水,细胞在打印结构中的铺展率有显著提高。通过优化挤出流量、打印移动速度和接收距离等细胞静电打印工艺参数后,打印结构分辨率达到30μm以下。细胞在该尺度下的凝胶结构中沿着纤维方向生长,表现出显著提高的细胞定向率。该功能化海藻酸钠墨水和细胞静电打印技术为精确复现自然组织的复杂微观结构提供了新的技术途径。

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