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On-line Access: 2022-12-24
Received: 2022-07-20
Revision Accepted: 2022-11-24
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Functionalized alginate-based bioinks for microscale electrohydrodynamic bioprinting of living tissue constructs with improved cellular spreading and alignment
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Zhennan Qiu, Hui Zhu, Yutao Wang, Ayiguli Kasimu, Dichen Li & Jiankang He. Functionalized alginate-based bioinks for microscale electrohydrodynamic bioprinting of living tissue constructs with improved cellular spreading and alignment[J]. Journal of Zhejiang University Science D, 2023, 6(2): 136-149.
@article{title="Functionalized alginate-based bioinks for microscale
electrohydrodynamic bioprinting of living tissue constructs
with improved cellular spreading and alignment",
author="Zhennan Qiu, Hui Zhu, Yutao Wang, Ayiguli Kasimu, Dichen Li & Jiankang He",
journal="Journal of Zhejiang University Science D",
volume="6",
number="2",
pages="136-149",
year="2023",
publisher="Zhejiang University Press & Springer",
doi="10.1007/s42242-022-00225-z"
}
%0 Journal Article
%T Functionalized alginate-based bioinks for microscale
electrohydrodynamic bioprinting of living tissue constructs
with improved cellular spreading and alignment
%A Zhennan Qiu
%A Hui Zhu
%A Yutao Wang
%A Ayiguli Kasimu
%A Dichen Li & Jiankang He
%J Journal of Zhejiang University SCIENCE D
%V 6
%N 2
%P 136-149
%@ 1869-1951
%D 2023
%I Zhejiang University Press & Springer
%DOI 10.1007/s42242-022-00225-z
TY - JOUR
T1 - Functionalized alginate-based bioinks for microscale
electrohydrodynamic bioprinting of living tissue constructs
with improved cellular spreading and alignment
A1 - Zhennan Qiu
A1 - Hui Zhu
A1 - Yutao Wang
A1 - Ayiguli Kasimu
A1 - Dichen Li & Jiankang He
J0 - Journal of Zhejiang University Science D
VL - 6
IS - 2
SP - 136
EP - 149
%@ 1869-1951
Y1 - 2023
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1007/s42242-022-00225-z
Abstract: Bioprinting has been widely investigated for tissue engineering and regenerative medicine applications. However, it is still
difficult to reconstruct the complex native cell arrangement due to the limited printing resolution of conventional bioprinting
techniques such as extrusion- and inkjet-based printing. Recently, an electrohydrodynamic (EHD) bioprinting strategy was
reported for the precise deposition of well-organized cell-laden constructs with microscale filament size, whereas few studies
have been devoted to developing bioinks that can be applied for EHD bioprinting and simultaneously support cell spreading.
This study describes functionalized alginate-based bioinks for microscale EHD bioprinting using peptide grafting and fibrin
incorporation, which leads to high cell viability (>90%) and cell spreading. The printed filaments can be further refined
to as small as 30 ?m by incorporating polyoxyethylene and remained stable over one week when exposed to an aqueous
environment. By utilizing the presented alginate-based bioinks, layer-specific cell alignment along the printing struts could
be observed inside the EHD-printed microscale filaments, which allows fabricating living constructs with cell-scale filament
resolution for guided cellular orientation.
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