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Abstract: This study of renaturation by dilution and size exclusion chromatography (SEC) addition of urea to improve yield as well as the initial and final protein concentrations showed that although urea decreased the rate of lysozyme refolding, it could suppress protein aggregation to sustain the pathway of correct refolding at high protein concentration; and that there existed an optimum urea concentration in renaturation buffer. Under the above conditions, lysozyme was successfully refolded from initial concentration of up to 40 mg/mL by dilution and 100 mg/mL by SEC, with the yield of the former being more than 40% and that of the latter being 34.8%. Especially, under the condition of 30 min interval time, i.e. τ>2(t_R2-t_R1), the efficiency was increased by 25% and the renaturation buffer could be recycled for SEC refolding in continuous operation of downstream process.

[1]Adkevich, V. I., Gutin, A. M. and Shakhnovich, E. I., 1994. Free energy landscape for protein folding kinetics, intermediates, traps and multiple pathway in theory and lattice model simulations. J. Chem. Phy.,101: 6052-6062.

[2]Batas, B. and Chaudhuri, J. B., 1996. Protein refolding at high concentration using size-exclusion chromatography. Biotech. Bioeng., 50: 16-23.

[3]Batas, B. and Chaudhuri, J. B., 1999. Consideration of sample application and elution during size-exclusion chromatography-based protein refolding. J. Chromatography A, 864: 229-236.

[4]Batas, B., Jones, H. R. and Chaudhuri J. B., 1997. Studies of the hydrodynamic volume changes that occur during refolding of lysozyme using size-exclusion chromatography. J. Chromatography A, 766: 109-119.

[5]Brandford, M. M., 1976. A rapid and sensitive method for the quantitation of protein utilizing the principles of protein-dye binding. Anal. Biochem., 72: 248-254.

[6]De Bernardez Clark, E., 2001. Protein refolding for industrial processes. Current Opinion in Biotechnology, 12: 202-207.

[7]Fahey, E. M. and Chaudhuri, J. B., 2000. Refolding of low molecular weight urokinase plasminogen activator by dilution and size exclusion chromatography-a comparative study. Separation Science and Technology, 35(11): 1743-1760.

[8]Goldberg, M. E., Rudolph, R. and Jaenicke R., 1991. A kinetic study of the competition between renaturation and aggregation during the refolding of denatured-reduced egg white lysozyme. Biochemistry, 30: 2790-2797.

[9]Gu, Z.Y., Su, Z.G. and Janson, J.C., 2001. Urea gradient size-exclusion chromatography enhanced the yield of lysozyme refolding. J. Chromatography A, 918: 311-318.

[10]Katoh, S., Katoh, Y., 2000. Continuous refolding of lysozyme with fed-batch addition of denatured protein solution. Process Biochemistry, 35: 1119-1124.

[11]Müller, C. and Rinas, U., 1999. Renaturation of heterodimeric platelet-derived growth factor from inclusion bodies of recombinant Escherichia coli using size-exclusion chromatography. J. Chromatography A, 855: 203-213.

[12]Rozema, D. and Gellman, S. H., 1996. Artificial chaperone-assisted refolding of denatured-reduced lysozyme: modulation of the competition between renaturation and aggregation. Biochemistry, 35: 15760-15771.

[13]Stellmach, B. and Qian, J.Y., 1992. Enzyme Measurement. China Light Industry Press, Beijing, China, p. 237-239.

[14]Zhang, Z., Tong K.T., Belew, M., Pettersson, T. and Janson, J.C., 1992. Production, purification and characterization of recombinant human interferon γ. J. Chromatography, 604: 143-155.