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Received: 2002-08-02
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Emergence of a new satellite RNA from cucumber mosaic virus isolate P1
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Sandra Pérez Alvarez, XUE Chao-yang, ZHOU Xue-ping. Emergence of a new satellite RNA from cucumber mosaic virus isolate P1[J]. Journal of Zhejiang University Science A, 2003, 4(3): 336-339.
@article{title="Emergence of a new satellite RNA from cucumber mosaic virus isolate P1",
author="Sandra Pérez Alvarez, XUE Chao-yang, ZHOU Xue-ping",
journal="Journal of Zhejiang University Science A",
volume="4",
number="3",
pages="336-339",
year="2003",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.2003.0336"
}
%0 Journal Article
%T Emergence of a new satellite RNA from cucumber mosaic virus isolate P1
%A Sandra Pé
%A rez Alvarez
%A XUE Chao-yang
%A ZHOU Xue-ping
%J Journal of Zhejiang University SCIENCE A
%V 4
%N 3
%P 336-339
%@ 1869-1951
%D 2003
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.2003.0336
TY - JOUR
T1 - Emergence of a new satellite RNA from cucumber mosaic virus isolate P1
A1 - Sandra Pé
A1 - rez Alvarez
A1 - XUE Chao-yang
A1 - ZHOU Xue-ping
J0 - Journal of Zhejiang University Science A
VL - 4
IS - 3
SP - 336
EP - 339
%@ 1869-1951
Y1 - 2003
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.2003.0336
Abstract: The cucumber mosaic virus (CMV) isolate P1 caused very mild symptoms on many plant species. After serial passages by mechanical inoculation over five years, CMV P1 caused severe symptoms on several tobacco cultivars and tomato. A specific band of approximately 0.3 kb in length was amplified by RT-PCR with primers synthesized based on reported CMV satellite RNA (satRNA) sequences. sequence analysis showed there were two satRNAs (Sat-P1-1 and Sat-P1-2). Sat-P1-1 contained 335 nucleotides, and Sat-P1-2 contained 394 nucleotides. These two satRNAs shared 64% overall nucleotide sequence homology, and differences between the two satRNAs included mutations as well as deletions. Sat-P1-1 was identical to a satRNA (Z96099) reported in 1995 in CMV P1. Based on differences in the sequence and secondary structure between these two satRNAs, we conclude that Sat-P1-2 represents the emergence of a new satellite (necrotic satellite) from attenuated satRNA populations. The possible effect of the emergence of this new satRNA is discussed.
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