JZUS - Journal of Zhejiang University SCIENCE

Journal of Zhejiang University SCIENCE B 2014 Vol.15 No.5 P.482-490

Adipose-derived stem cells transfected with pEGFP-OSX enhance bone formation during distraction osteogenesis*

Author(s): Qing-guo Lai^1,2, Shao-long Sun^3,4, Xiao-hong Zhou⁵, Chen-ping Zhang², Kui-feng Yuan¹, Zhong-jun Yang¹, Sheng-lei Luo¹, Xiao-peng Tang¹, Jiang-bo Ci¹
Affiliation(s): 1. Department of Oral and Maxillofacial Surgery, the Second Hospital of Shandong University, Jinan 250033, China; more
Corresponding email(s): zhangchenping9yuan@126.com
Key Words: Adipose-derived stem cell (ADSC), Distraction osteogenesis, Transcription factor, Gene transfection

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Qing-guo Lai, Shao-long Sun, Xiao-hong Zhou, Chen-ping Zhang, Kui-feng Yuan, Zhong-jun Yang, Sheng-lei Luo, Xiao-peng Tang, Jiang-bo Ci. Adipose-derived stem cells transfected with pEGFP-OSX enhance bone formation during distraction osteogenesis[J]. Journal of Zhejiang University Science B, 2014, 15(5): 482-490.

@article{title="Adipose-derived stem cells transfected with pEGFP-OSX enhance bone formation during distraction osteogenesis",
author="Qing-guo Lai, Shao-long Sun, Xiao-hong Zhou, Chen-ping Zhang, Kui-feng Yuan, Zhong-jun Yang, Sheng-lei Luo, Xiao-peng Tang, Jiang-bo Ci",
journal="Journal of Zhejiang University Science B",
volume="15",
number="5",
pages="482-490",
year="2014",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1300203"
}

%0 Journal Article
%T Adipose-derived stem cells transfected with pEGFP-OSX enhance bone formation during distraction osteogenesis
%A Qing-guo Lai
%A Shao-long Sun
%A Xiao-hong Zhou
%A Chen-ping Zhang
%A Kui-feng Yuan
%A Zhong-jun Yang
%A Sheng-lei Luo
%A Xiao-peng Tang
%A Jiang-bo Ci
%J Journal of Zhejiang University SCIENCE B
%V 15
%N 5
%P 482-490
%@ 1673-1581
%D 2014
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1300203

TY - JOUR
T1 - Adipose-derived stem cells transfected with pEGFP-OSX enhance bone formation during distraction osteogenesis
A1 - Qing-guo Lai
A1 - Shao-long Sun
A1 - Xiao-hong Zhou
A1 - Chen-ping Zhang
A1 - Kui-feng Yuan
A1 - Zhong-jun Yang
A1 - Sheng-lei Luo
A1 - Xiao-peng Tang
A1 - Jiang-bo Ci
J0 - Journal of Zhejiang University Science B
VL - 15
IS - 5
SP - 482
EP - 490
%@ 1673-1581
Y1 - 2014
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1300203

Abstract
Chinese Summary
Academic Network
Reviewer Comment

Abstract: This study was designed to investigate the effects of local delivery of adipose-derived stem cells (ADSCs) transfected with transcription factor osterix (OSX) on bone formation during distraction osteogenesis. New Zealand white rabbits (n=54) were randomly divided into three groups (18 rabbits per group). A directed cloning technique was used for the construction of recombinant plasmid pEGFP-OSX, where EGFP is the enhanced green fluorescence protein. After osteodistraction of the right mandible of all experimental rabbits, rabbits in group A were treated with ADSCs transfected with pEGFP-OSX, group B with ADSCs transfected with pEGFP-N1, and group C with physiological saline. Radiographic and histological examinations were processed after half of the animals within each group were humanely killed by injection of sodium pentothal at Week 2 or 6 after surgery. The distraction bone density was measured as its projectional bone mineral density (BMD). Three parameters were measured, namely, the thickness of new trabeculae (TNT), and the volumes of the newly generated cortical bone (NBV1) and the cancellous bone (NBV2) of the distracted regions. Good bone generation in the distraction areas was found in group A, which had the highest BMD, TNT, and NBV in the distraction zones among the groups. There was no significant difference in bone generation in the distraction areas between groups B and C. The results indicate that the transplantation of ADSCs transfected with pEGFP-OSX can effectively promote bone generation during distraction in vivo.

pEGFP-OSX修饰的脂肪干细胞促进牵张成骨中新骨形成

研究目的：观察牵张间隙植入pEGFP-OSX修饰的脂肪干细胞对牵张成骨中新骨形成的作用。
创新要点：提出来源广泛容易获取的脂肪干细胞作为种子细胞，被基因OSX修饰后，在体内环境下可能促进成骨。
研究方法：通过X射线检查、组织学检查等手段观察成骨效果，进行统计学分析。
重要结论：通过短期的动物试验发现，pEGFP-OSX修饰的脂肪干细胞在体内环境下促进牵张成骨中新骨形成（见表1和图3-5）。

关键词：脂肪干细胞；牵张成骨；转录因子；基因转染

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pEGFP-OSX修饰的脂肪干细胞促进牵张成骨中新骨形成

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