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On-line Access: 2010-07-04

Received: 2009-12-27

Revision Accepted: 2010-04-07

Crosschecked: 2010-06-13

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Journal of Zhejiang University SCIENCE B 2010 Vol.11 No.7 P.489-496


All-trans retinoic acid promotes smooth muscle cell differentiation of rabbit bone marrow-derived mesenchymal stem cells

Author(s):  Zhong-yuan Su, Ying Li, Xiao-li Zhao, Ming Zhang

Affiliation(s):  College of Life Sciences, Zhejiang University, Hangzhou 310058, China, Zhejiang Academy of Medical Science, Hangzhou 310058, China

Corresponding email(s):   zhangming_ls@zju.edu.cn

Key Words:  Bone marrow-derived mesenchymal stem cells, Smooth muscle cells, All-trans retinoic acid, Rabbit

Zhong-yuan Su, Ying Li, Xiao-li Zhao, Ming Zhang. All-trans retinoic acid promotes smooth muscle cell differentiation of rabbit bone marrow-derived mesenchymal stem cells[J]. Journal of Zhejiang University Science B, 2010, 11(7): 489-496.

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author="Zhong-yuan Su, Ying Li, Xiao-li Zhao, Ming Zhang",
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publisher="Zhejiang University Press & Springer",

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%T All-trans retinoic acid promotes smooth muscle cell differentiation of rabbit bone marrow-derived mesenchymal stem cells
%A Zhong-yuan Su
%A Ying Li
%A Xiao-li Zhao
%A Ming Zhang
%J Journal of Zhejiang University SCIENCE B
%V 11
%N 7
%P 489-496
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%D 2010
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B0900415

T1 - All-trans retinoic acid promotes smooth muscle cell differentiation of rabbit bone marrow-derived mesenchymal stem cells
A1 - Zhong-yuan Su
A1 - Ying Li
A1 - Xiao-li Zhao
A1 - Ming Zhang
J0 - Journal of Zhejiang University Science B
VL - 11
IS - 7
SP - 489
EP - 496
%@ 1673-1581
Y1 - 2010
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B0900415

bone marrow-derived mesenchymal stem cells are multipotent stem cells, an attractive resource for regenerative medicine. Accumulating evidence suggests that all-trans retinoic acid plays a key role in the development and differentiation of smooth muscle cells. In the present study, we demonstrate, for the first time, that rabbit bone marrow-derived mesenchymal stem cells differentiate into smooth muscle cells upon the treatment with all-trans retinoic acid. all-trans retinoic acid increased the expression of myocardin, caldesmon, 22-kDa smooth muscle cell-specific protein (SM22α), and SM-myosin heavy chains in rabbit bone marrow-derived mesenchymal stem cells, as detected by reverse transcription polymerase chain reaction (PCR). Immunostaining of SM22α and SM-myosin heavy chains using monoclonal antibodies also indicated smooth muscle cell differentiation of rabbit bone marrow-derived mesenchymal stem cells following the treatment with all-trans retinoic acid. In addition, more than 47% of bone marrow-derived mesenchymal stem cells demonstrated the contractile phenotype of smooth muscle cells. Western blot results showed that SM-1 and SM-2 were highly expressed in the differentiated cells. These results suggest that all-trans retinoic acid may serve as a potent agent for functional smooth muscle cell differentiation in tissue engineering.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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