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CLC number: R285.5

On-line Access: 2017-05-04

Received: 2016-08-09

Revision Accepted: 2017-01-15

Crosschecked: 2017-04-10

Cited: 2

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Journal of Zhejiang University SCIENCE B 2017 Vol.18 No.5 P.445-448

http://doi.org/10.1631/jzus.B1600355


Ginsenoside Rg1 promotes neural differentiation of mouse adipose-derived stem cells via the miRNA-124 signaling pathway


Author(s):  Juan Dong, Guo Zhu, Tian-cheng Wang, Fu-shan Shi

Affiliation(s):  Department of Veterinary Medicine, College of Animal Sciences, Zhejiang University, Hangzhou 310058, China; more

Corresponding email(s):   sfs@zju.edu.cn

Key Words:  Adipose-derived stem cells (ADSC), Ginsenoside Rg1, Surgical treatment


Juan Dong, Guo Zhu, Tian-cheng Wang, Fu-shan Shi. Ginsenoside Rg1 promotes neural differentiation of mouse adipose-derived stem cells via the miRNA-124 signaling pathway[J]. Journal of Zhejiang University Science B, 2017, 18(5): 445-448.

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author="Juan Dong, Guo Zhu, Tian-cheng Wang, Fu-shan Shi",
journal="Journal of Zhejiang University Science B",
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pages="445-448",
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publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1600355"
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%T Ginsenoside Rg1 promotes neural differentiation of mouse adipose-derived stem cells via the miRNA-124 signaling pathway
%A Juan Dong
%A Guo Zhu
%A Tian-cheng Wang
%A Fu-shan Shi
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T1 - Ginsenoside Rg1 promotes neural differentiation of mouse adipose-derived stem cells via the miRNA-124 signaling pathway
A1 - Juan Dong
A1 - Guo Zhu
A1 - Tian-cheng Wang
A1 - Fu-shan Shi
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SP - 445
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PB - Zhejiang University Press & Springer
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DOI - 10.1631/jzus.B1600355


Abstract: 
We have explored the role of ginsenoside Rg1 in promoting the differentiation of mouse adipose-derived stem cells (mADSC) towards the neuronal lineage. The central nervous system has long been regarded as incapable of self-repair; therefore neuronal differentiation from stem cells is of great interest. However, the use of embryonic stem cells is limited due to their inaccessibility and for ethical reasons, so the search is on for alternative pluripotent cells capable of differentiating into neuronal cells. adipose-derived stem cells (ADSC) can differentiate into different cell types, including neuronal cells: their accessibility, low risk, and capacity for long-term growth and self-renewal have made them the preferred stem cell type for clinical applications. Several methods have been indicated for promoting the neuronal differentiation of ADSC, but the mechanism of this process has not been clearly identified. As our previous study showed that microRNA-124 (miRNA-124) plays a positive role in promoting the neural differentiation of ADSC, we wanted to find reagents that can upregulate miRNA-124 expression during neural differentiation.

人参皂甙Rg1通过miRNA-124途径促进小鼠脂肪干细胞向神经样细胞分化

目的:研究人参皂甙Rg1对小鼠脂肪干细胞神经样分化的促进作用,并初步探讨其作用机理。
创新点:证明了人参皂甙Rg1可以通过miRNA-124途径促进脂肪干细胞的神经样分化。
方法:从BALB/c小鼠腹股沟和睾丸脂肪垫处分离培养脂肪干细胞,利用流式细胞仪检测分离的脂肪干细胞纯度。试验分为以下五组:磷酸缓冲盐溶液(PBS)组、3-异丁基-1-甲基黄嘌呤(IBMX)组、IBMX+Rg1低剂量组、IBMX+Rg1中剂量组和IBMX+Rg1高剂量组。用细胞免疫组化方法检测了脂肪干细胞向神经样细胞的分化效率,用荧光定量聚合酶链式反应(qPCR)方法检测miRNA-124的表达变化,用免疫印迹的方法检测巢蛋白(nestin)、βIII-微管蛋白(βIII-tubulin)及羧基端小结构域磷酸酶1(SCP1)的表达水平。
结论:免疫组化结果显示,IBMX可以成功诱导小鼠脂肪干细胞向神经样细胞的分化;免疫印迹结果显示,Rg1可以显著提高神经样细胞标记蛋白的表达水平;荧光定量PCR结果显示,Rg1可以促进miRNA-124的表达量,进而降解神经分化抑制因子SCP1的表达,促进脂肪干细胞的神经样分化效率。

关键词:脂肪干细胞;人参皂甙Rg1;神经样分化

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Reference

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