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CLC number: R28
On-line Access: 2014-11-04
Received: 2014-02-10
Revision Accepted: 2014-08-04
Crosschecked: 2014-10-15
Cited: 2
Clicked: 5919
Simultaneous determination by UPLC-MS/MS of seven bioactive compounds in rat plasma after oral administration of Ginkgo biloba tablets: application to a pharmacokinetic study
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Wen-ping Wang, Na Liu, Qian Kang, Pei-pei Du, Yi Lan, Bo-chen Zhao, Yan-yan Chen, Qing Zhang, Hui Li, Ye-wen Zhang, Qing Wu. Simultaneous determination by UPLC-MS/MS of seven bioactive compounds in rat plasma after oral administration of Ginkgo biloba tablets: application to a pharmacokinetic study[J]. Journal of Zhejiang University Science B, 2014, 15(11): 929-939.
@article{title="Simultaneous determination by UPLC-MS/MS of seven bioactive compounds in rat plasma after oral administration of Ginkgo biloba tablets: application to a pharmacokinetic study",
author="Wen-ping Wang, Na Liu, Qian Kang, Pei-pei Du, Yi Lan, Bo-chen Zhao, Yan-yan Chen, Qing Zhang, Hui Li, Ye-wen Zhang, Qing Wu",
journal="Journal of Zhejiang University Science B",
volume="15",
number="11",
pages="929-939",
year="2014",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1400035"
}
%0 Journal Article
%T Simultaneous determination by UPLC-MS/MS of seven bioactive compounds in rat plasma after oral administration of Ginkgo biloba tablets: application to a pharmacokinetic study
%A Wen-ping Wang
%A Na Liu
%A Qian Kang
%A Pei-pei Du
%A Yi Lan
%A Bo-chen Zhao
%A Yan-yan Chen
%A Qing Zhang
%A Hui Li
%A Ye-wen Zhang
%A Qing Wu
%J Journal of Zhejiang University SCIENCE B
%V 15
%N 11
%P 929-939
%@ 1673-1581
%D 2014
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1400035
TY - JOUR
T1 - Simultaneous determination by UPLC-MS/MS of seven bioactive compounds in rat plasma after oral administration of Ginkgo biloba tablets: application to a pharmacokinetic study
A1 - Wen-ping Wang
A1 - Na Liu
A1 - Qian Kang
A1 - Pei-pei Du
A1 - Yi Lan
A1 - Bo-chen Zhao
A1 - Yan-yan Chen
A1 - Qing Zhang
A1 - Hui Li
A1 - Ye-wen Zhang
A1 - Qing Wu
J0 - Journal of Zhejiang University Science B
VL - 15
IS - 11
SP - 929
EP - 939
%@ 1673-1581
Y1 - 2014
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1400035
Abstract: A rapid, reliable, and sensitive method was developed using ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) with an electrospray ionization (ESI) source for determination of seven bioactive compounds in rat plasma after oral administration of Ginkgo biloba tablets (GBTs). The method simultaneously detects bilobalide (BB), ginkgolide A (GA), ginkgolide B (GB), ginkgolide C (GC), quercetin (QCT), kaempferol (KMF), and isorhamnetin (ISR) for pharmacokinetic study. The analytes and internal standard (IS) were extracted from rat plasma by acetidin. An MS/MS detection was conducted using multiple reaction monitoring (MRM) and operating in the negative ionization mode. The calibration curve ranges were 5–500, 5–500, 2.5–250, 1–100, 1–100, 1–100, and 1–100 ng/ml for BB, GA, GB, GC, QCT, KMF, and ISR, respectively. The mean recovery of the analytes ranged from 68.11% to 84.42%. The intra- and inter-day precisions were in the range of 2.33%–9.86% and the accuracies were between 87.67% and 108.37%. The method was used successfully in a pharmacokinetic study of GBTs. The pharmacokinetic parameters of seven compounds were analyzed using a non-compartment model. Plasma concentrations of the seven compounds were determined up to 48 h after administration, and their pharmacokinetic parameters were in agreement with previous studies.
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