CLC number: S571.1
On-line Access: 2024-08-27
Received: 2023-10-17
Revision Accepted: 2024-05-08
Crosschecked: 2019-05-31
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Wen-jie Zhang, Cong Liu, Rui-juan Yang, Ting-ting Zheng, Miao-miao Zhao, Li Ma, Liang Yan. Comparison of volatile profiles and bioactive components of sun-dried Pu-erh tea leaves from ancient tea plants on Bulang Mountain measured by GC-MS and HPLC[J]. Journal of Zhejiang University Science B, 2019, 20(7): 563-575.
@article{title="Comparison of volatile profiles and bioactive components of sun-dried Pu-erh tea leaves from ancient tea plants on Bulang Mountain measured by GC-MS and HPLC",
author="Wen-jie Zhang, Cong Liu, Rui-juan Yang, Ting-ting Zheng, Miao-miao Zhao, Li Ma, Liang Yan",
journal="Journal of Zhejiang University Science B",
volume="20",
number="7",
pages="563-575",
year="2019",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1800183"
}
%0 Journal Article
%T Comparison of volatile profiles and bioactive components of sun-dried Pu-erh tea leaves from ancient tea plants on Bulang Mountain measured by GC-MS and HPLC
%A Wen-jie Zhang
%A Cong Liu
%A Rui-juan Yang
%A Ting-ting Zheng
%A Miao-miao Zhao
%A Li Ma
%A Liang Yan
%J Journal of Zhejiang University SCIENCE B
%V 20
%N 7
%P 563-575
%@ 1673-1581
%D 2019
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1800183
TY - JOUR
T1 - Comparison of volatile profiles and bioactive components of sun-dried Pu-erh tea leaves from ancient tea plants on Bulang Mountain measured by GC-MS and HPLC
A1 - Wen-jie Zhang
A1 - Cong Liu
A1 - Rui-juan Yang
A1 - Ting-ting Zheng
A1 - Miao-miao Zhao
A1 - Li Ma
A1 - Liang Yan
J0 - Journal of Zhejiang University Science B
VL - 20
IS - 7
SP - 563
EP - 575
%@ 1673-1581
Y1 - 2019
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1800183
Abstract: To explore the volatile profiles and the contents of ten bioactive components (polyphenols and caffeine) of sun-dried Pu-erh tea leaves from ancient tea plants on bulang Mountain, 17 samples of three tea varieties were analyzed by headspace-solid phase microextraction-gas chromatography-mass spectrometry (HS-SPME-GC-MS) and high-performance liquid chromatography (HPLC). A total of 75 volatile components were tentatively identified. Laomaner (LME), Laobanzhang (LBZ), and other teas on bulang Mountain (BL) contained 70, 53, and 71 volatile compounds, respectively. Among the volatile compounds, alcohols (30.2%–45.8%), hydrocarbons (13.7%–17.5%), and ketones (12.4%–23.4%) were qualitatively the most dominant volatile compounds in the different tea varieties. The average content of polyphenol was highest in LME (102.1 mg/g), followed by BL (98.7 mg/g) and LBZ (88.0 mg/g), while caffeine showed the opposite trend, 27.3 mg/g in LME, 33.5 mg/g in BL, and 38.1 mg/g in LBZ. Principal component analysis applied to both the volatile compounds and ten bioactive components showed a poor separation of samples according to varieties, while partial least squares-discriminant analysis (PLS-DA) showed satisfactory discrimination. Thirty-four volatile components and five bioactive compounds were selected as major discriminators (variable importance in projection (VIP) >1) among the tea varieties. These results suggest that chromatographic data combined with multivariate analysis could provide a useful technique to characterize and distinguish the sun-dried Pu-erh tea leaves from ancient tea varieties on bulang Mountain.
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[32]List of electronic supplementary materials
[33]Fig. S1 Total ion chromatograms of the intra-day repeatability experiments
[34]Fig. S2 VIP plots of PLS-DA based on volatiles data and bioactive compounds data
[35]Table S1 Detail results of intra-day repeatability of the HS-SPME method
[36]Table S2 Volatile compounds in seventeen tea samples
[37]Table S3 Contents of ten bioactive compounds in seventeen tea samples
[38]Table S4 Compound list with VIP value larger than 1.0
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