Full Text:   <2605>

Summary:  <1891>

CLC number: R737.31

On-line Access: 2024-08-27

Received: 2023-10-17

Revision Accepted: 2024-05-08

Crosschecked: 2020-03-03

Cited: 0

Clicked: 4430

Citations:  Bibtex RefMan EndNote GB/T7714

 ORCID:

Lin Deng

https://orcid.org/0000-0002-3648-8066

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Journal of Zhejiang University SCIENCE B 2020 Vol.21 No.4 P.315-326

http://doi.org/10.1631/jzus.B1900445


Cyclooxygenase-2 promotes ovarian cancer cell migration and cisplatin resistance via regulating epithelial mesenchymal transition


Author(s):  Lin Deng, Ding-qing Feng, Bin Ling

Affiliation(s):  China-Japan Friendship Hospital, Beijing 100029, China; more

Corresponding email(s):   lingbin.ling@vip.sina.com

Key Words:  Ovarian cancer (OC), Cyclooxygenase-2 (COX-2), Drug resistance, Migration, Epithelial-mesenchymal transition (EMT)



Abstract: 
Objective: Drug-resistance and metastasis are major reasons for the high mortality of ovarian cancer (OC) patients. cyclooxygenase-2 (COX-2) plays a critical role in OC development. This study was designed to evaluate the effects of COX-2 on migration and cisplatin (cis-dichloro diammine platinum, CDDP) resistance of OC cells and explore its related mechanisms. Methods: Cell counting kit-8 (CCK-8) assay was used to detect the cytotoxicity effects of celecoxib (CXB) and CDDP on SKOV3 and ES2 cells. The effect of COX-2 on migration was evaluated via the healing test. Western blot and real-time quantitative polymerase chain reaction (qPCR) were used to analyze E-cadherin, vimentin, Snail, and Slug levels. Results: COX-2 promoted drug-resistance and cell migration. CXB inhibited these effects. The combination of CDDP and CXB increased tumor cell sensitivity, reduced the amount of CDDP required, and shortened treatment administration time. COX-2 upregulation increased the expression of Snail and Slug, resulting in E-cadherin expression downregulation and vimentin upregulation. Conclusions: COX-2 promotes cancer cell migration and CDDP resistance and may serve as a potential target for curing OC.

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