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Pig meniscus single-cell sequencing reveals highly active red zone chondrocyte populations involved in stemness maintenance and vascularization development
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Monika MANKOWSKA, Monika STEFANSKA, Anna Maria MLECZKO, Katarzyna SARAD, Witold KOT, Lukasz KRYCH, Julia Anna SEMBA, Eric Lars-Helge LINDBERG, Jakub Dalibor RYBKA. Pig meniscus single-cell sequencing reveals highly active red zone chondrocyte populations involved in stemness maintenance and vascularization development[J]. Journal of Zhejiang University Science B, 1998, -1(-1): .
@article{title="Pig meniscus single-cell sequencing reveals highly active red zone chondrocyte populations involved in stemness maintenance and vascularization development",
author="Monika MANKOWSKA, Monika STEFANSKA, Anna Maria MLECZKO, Katarzyna SARAD, Witold KOT, Lukasz KRYCH, Julia Anna SEMBA, Eric Lars-Helge LINDBERG, Jakub Dalibor RYBKA",
journal="Journal of Zhejiang University Science B",
volume="-1",
number="-1",
pages="",
year="1998",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B2400388"
}
%0 Journal Article
%T Pig meniscus single-cell sequencing reveals highly active red zone chondrocyte populations involved in stemness maintenance and vascularization development
%A Monika MANKOWSKA
%A Monika STEFANSKA
%A Anna Maria MLECZKO
%A Katarzyna SARAD
%A Witold KOT
%A Lukasz KRYCH
%A Julia Anna SEMBA
%A Eric Lars-Helge LINDBERG
%A Jakub Dalibor RYBKA
%J Journal of Zhejiang University SCIENCE B
%V -1
%N -1
%P
%@ 1673-1581
%D 1998
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B2400388
TY - JOUR
T1 - Pig meniscus single-cell sequencing reveals highly active red zone chondrocyte populations involved in stemness maintenance and vascularization development
A1 - Monika MANKOWSKA
A1 - Monika STEFANSKA
A1 - Anna Maria MLECZKO
A1 - Katarzyna SARAD
A1 - Witold KOT
A1 - Lukasz KRYCH
A1 - Julia Anna SEMBA
A1 - Eric Lars-Helge LINDBERG
A1 - Jakub Dalibor RYBKA
J0 - Journal of Zhejiang University Science B
VL - -1
IS - -1
SP -
EP -
%@ 1673-1581
Y1 - 1998
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B2400388
Abstract: meniscus injuries are widespread and the available treatments do not offer enough healing potential. Here we provide critical support for the use of pigs as a biological model for meniscal degeneration and the development of cutting-edge therapies in orthopaedics. We present a single-cell transcriptome atlas of the meniscus, consisting of cell clusters corresponding to four major cell types: chondrocytes, endothelial cells, smooth muscle cells, and immune cells. Five distinct chondrocyte subclusters (Ch0-Ch4) were annotated, of which only one was widespread in both the red and white zones, indicating a major difference in the cellular makeup of the zones. Subclusters distinctive to the white zone appear to be responsible for cartilage-specific matrix deposition and protection against adverse microenvironmental factors while those in the red zone exhibit characteristics of mesenchymal stem cells and are more likely to proliferate and migrate. Additionally, they induce remodelling actions in other chondrocyte subclusters and promote the proliferation and maturation of endothelial cells, inducing healing and vascularization processes. Considering they have substantial remodelling capabilities, these subclusters should be of great interest for tissue engineering studies. We also show that the cellular makeup of the pig meniscus is comparable to that of humans, which supports the use of pigs as a model in orthopaedic therapy development.
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