Full Text:   <1050>

CLC number: 

On-line Access: 2021-01-11

Received: 2020-09-02

Revision Accepted: 2020-12-01

Crosschecked: 0000-00-00

Cited: 0

Clicked: 1534

Citations:  Bibtex RefMan EndNote GB/T7714

-   Go to

Article info.
Open peer comments

Bio-Design and Manufacturing  2021 Vol.4 No.2 P.203-221

http://doi.org/10.1007/s42242-020-00118-z


A novel bio-active microsphere for meniscus regeneration via inducing cell migration and chondrocyte differentiation


Author(s):  Hongyao Xu, He Huang, Xiangjie Zou, Pengcheng Xia, Warren A. L. S. Foon & Jinwen Wang

Affiliation(s):  Department of Sports Medicine and Joint Surgery, Nanjing First Hospital, Nanjing Medical University, 68 Changle Road, Nanjing 210006, Jiangsu, China

Corresponding email(s):   riversh922@hotmail.com

Key Words:  Fully reduced HMGB1 (frHMGB1), Kartogenin, Alginate microsphere, Cell migration, Meniscus regeneration


Share this article to: More

Hongyao Xu, He Huang, Xiangjie Zou, Pengcheng Xia, Warren A. L. S. Foon & Jinwen Wang . A novel bio-active microsphere for meniscus regeneration via inducing cell migration and chondrocyte differentiation[J]. Journal of Zhejiang University Science D, 2021, 4(2): 203-221.

@article{title="A novel bio-active microsphere for meniscus regeneration via inducing cell migration and chondrocyte differentiation",
author="Hongyao Xu, He Huang, Xiangjie Zou, Pengcheng Xia, Warren A. L. S. Foon & Jinwen Wang ",
journal="Journal of Zhejiang University Science D",
volume="4",
number="2",
pages="203-221",
year="2021",
publisher="Zhejiang University Press & Springer",
doi="10.1007/s42242-020-00118-z"
}

%0 Journal Article
%T A novel bio-active microsphere for meniscus regeneration via inducing cell migration and chondrocyte differentiation
%A Hongyao Xu
%A He Huang
%A Xiangjie Zou
%A Pengcheng Xia
%A Warren A. L. S. Foon & Jinwen Wang
%J Journal of Zhejiang University SCIENCE D
%V 4
%N 2
%P 203-221
%@ 1869-1951
%D 2021
%I Zhejiang University Press & Springer
%DOI 10.1007/s42242-020-00118-z

TY - JOUR
T1 - A novel bio-active microsphere for meniscus regeneration via inducing cell migration and chondrocyte differentiation
A1 - Hongyao Xu
A1 - He Huang
A1 - Xiangjie Zou
A1 - Pengcheng Xia
A1 - Warren A. L. S. Foon & Jinwen Wang
J0 - Journal of Zhejiang University Science D
VL - 4
IS - 2
SP - 203
EP - 221
%@ 1869-1951
Y1 - 2021
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1007/s42242-020-00118-z


Abstract: 
Meniscus injury is a common disease in clinic. If it was not treated in time, it leads to osteoarthritis which brings unbearable pain and heavy economic burden to the patients. At present, meniscectomy and meniscus suture are widely used in the treatment for meniscus injury. Nevertheless, It is not ideal for poor self-healing ability of meniscus. The recruitment of endogenous stem cells is an attractive option for wounded meniscus healing. Fully reduced high-mobility group box 1 protein (HMGB1) can accelerate the regeneration of multiple tissues by endogenous stem cell activation, migration and differentiation. kartogenin (KGN) has shown to induce the chondrogenesis of the stem cells. However, no study has explored such effects of HMGB1 and KGN in wounded meniscus healing. Therefore, in order to improve the regeneration of meniscus, we intend to use a novel bioactive microsphere which was developed by combining fully reduced high mobility group box 1 (frHMGB1) and kartogenin (KGN) with alginate gel which slowly release high concentrations of HMGB1 and KGN to activate rat bone marrow stem cells (BMSCs) and promote cell proliferation. The results showed that this HMGB1–KGN microsphere released and kept high concentrations of HMGB1 and KGN in the wound area for more than 2 weeks. In vitro experimental results showed that the HMGB1–KGN microsphere can promote cell proliferation via recruiting rat bone marrow stem cells (BMSCs) and activating the BMSCs from G0 to GAlert stage as evidenced by cell migration testing and 5-bromo-2′-deoxyuridine (BrdU) incorporation assay. In vivo results indicated that this HMGB-KGN microsphere can recruit GFP-labeled BMSCs from tail vein to wounded meniscus and induce these GFP-labeled BMSCs to differentiate into chondrocytes. Our results demonstrated that the HMGB1–KGN-containing bioactive microsphere induced cell migration in vitro and recruited the cells to wound area to promote wounded rat meniscus

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

Open peer comments: Debate/Discuss/Question/Opinion

<1>

Please provide your name, email address and a comment





Journal of Zhejiang University-SCIENCE, 38 Zheda Road, Hangzhou 310027, China
Tel: +86-571-87952783; E-mail: cjzhang@zju.edu.cn
Copyright © 2000 - 2022 Journal of Zhejiang University-SCIENCE