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Journal of Zhejiang University SCIENCE A 2003 Vol.4 No.3 P.336-339

http://doi.org/10.1631/jzus.2003.0336


Emergence of a new satellite RNA from cucumber mosaic virus isolate P1


Author(s):  Sandra Pé,rez Alvarez, XUE Chao-yang, ZHOU Xue-ping

Affiliation(s):  Institute of Biotechnology, Zhejiang University, Hangzhou 310029, China

Corresponding email(s):   sandraperez_2000@hotmail.com, zzhou@zju.edu.cn

Key Words:  Cucumber mosaic virus, Satellite RNA, Sequence


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Sandra Pérez Alvarez, XUE Chao-yang, ZHOU Xue-ping. Emergence of a new satellite RNA from cucumber mosaic virus isolate P1[J]. Journal of Zhejiang University Science A, 2003, 4(3): 336-339.

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Abstract: 
The cucumber mosaic virus (CMV) isolate P1 caused very mild symptoms on many plant species. After serial passages by mechanical inoculation over five years, CMV P1 caused severe symptoms on several tobacco cultivars and tomato. A specific band of approximately 0.3 kb in length was amplified by RT-PCR with primers synthesized based on reported CMV satellite RNA (satRNA) sequences. sequence analysis showed there were two satRNAs (Sat-P1-1 and Sat-P1-2). Sat-P1-1 contained 335 nucleotides, and Sat-P1-2 contained 394 nucleotides. These two satRNAs shared 64% overall nucleotide sequence homology, and differences between the two satRNAs included mutations as well as deletions. Sat-P1-1 was identical to a satRNA (Z96099) reported in 1995 in CMV P1. Based on differences in the sequence and secondary structure between these two satRNAs, we conclude that Sat-P1-2 represents the emergence of a new satellite (necrotic satellite) from attenuated satRNA populations. The possible effect of the emergence of this new satRNA is discussed.

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Reference

[1]Cheng, N. H., Fang, R. X., Pu, Z. Q. and Fang, Z. D., 1997. Complete nucleotide sequence of a new satellite RNA associated with cucumber mosaic virus inducing tomato necrosis. Science in China Series C, 40: 443-448.

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[3]Grieco, F., Lanave, C. and Gallitelli, D., 1997. Evolutionary dynamics of cucumber mosaic virus satellite RNA during natural epidemics in Italy. Virology, 229: 166-174.

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[7]Zhou, X. P., Xu, Z. X., Xu, J. and Li, D. B., 1995b. Study on cucumber mosaic virus infecting Luffer cylidrica. Journal of South China Agriculture University, 16(2): 74-79.

[8]Zhou, X. P., Xue, C. Y., Chen, Q., Qi, Y. J. and Li, D. B., 2000. Complete nucleotide sequence and genome organization of tobacco mosaic virus isolated from Vicia faba. Science in China Series C, 43: 200-208.

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