CLC number: Q813.4; Q78; Q939.13
On-line Access: 2024-08-27
Received: 2023-10-17
Revision Accepted: 2024-05-08
Crosschecked: 0000-00-00
Cited: 12
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LI Yong-quan, CEN Pei-lin, CHEN Shi-fei, WU Dan, ZHENG Jing. A pair of two-component regulatory genes ecrA1/A2 in S. coelicolor[J]. Journal of Zhejiang University Science A, 2004, 5(2): 173-179.
@article{title="A pair of two-component regulatory genes ecrA1/A2 in S. coelicolor",
author="LI Yong-quan, CEN Pei-lin, CHEN Shi-fei, WU Dan, ZHENG Jing",
journal="Journal of Zhejiang University Science A",
volume="5",
number="2",
pages="173-179",
year="2004",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.2004.0173"
}
%0 Journal Article
%T A pair of two-component regulatory genes ecrA1/A2 in S. coelicolor
%A LI Yong-quan
%A CEN Pei-lin
%A CHEN Shi-fei
%A WU Dan
%A ZHENG Jing
%J Journal of Zhejiang University SCIENCE A
%V 5
%N 2
%P 173-179
%@ 1869-1951
%D 2004
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.2004.0173
TY - JOUR
T1 - A pair of two-component regulatory genes ecrA1/A2 in S. coelicolor
A1 - LI Yong-quan
A1 - CEN Pei-lin
A1 - CHEN Shi-fei
A1 - WU Dan
A1 - ZHENG Jing
J0 - Journal of Zhejiang University Science A
VL - 5
IS - 2
SP - 173
EP - 179
%@ 1869-1951
Y1 - 2004
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.2004.0173
Abstract: Two-component genes are kinds of genetic elements involved in regulation of antibiotic production in Streptomyces coelicolor. DNA microarray analysis revealed thatecr A1/A2, which mapped at distant sites from red locus and encode respectively the kinase and regulator, expressed coordinately with genes of Red specific biosynthetic pathway. ecrA1 and ecrA2 gene-disruptive mutants were constructed using homogenotisation by reciprocal double crossover. Fermentation data showed that the undecylprodigiosin (Red) level of production was lower than that of wild-type strain. However, the change of the actinorhodin (Act) production level was not significant compared with wild type. Thus, these experiment results confirmed that the two-component system ecrA1/A2 was positive regulatory element for red gene cluster.
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