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Journal of Zhejiang University SCIENCE A 2004 Vol.5 No.2 P.173-179

http://doi.org/10.1631/jzus.2004.0173


A pair of two-component regulatory genes ecrA1/A2 in S. coelicolor


Author(s):  LI Yong-quan, CEN Pei-lin, CHEN Shi-fei, WU Dan, ZHENG Jing

Affiliation(s):  College of Life Science, Zhejiang University, Hongzhou 310027, China; more

Corresponding email(s):   lyq@mail.hz.zj.cn

Key Words:  Streptomyces coelicolor, Two-component system, Antibiotic gene cluster, ecrA1/A2


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LI Yong-quan, CEN Pei-lin, CHEN Shi-fei, WU Dan, ZHENG Jing. A pair of two-component regulatory genes ecrA1/A2 in S. coelicolor[J]. Journal of Zhejiang University Science A, 2004, 5(2): 173-179.

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%A LI Yong-quan
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%A CHEN Shi-fei
%A WU Dan
%A ZHENG Jing
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T1 - A pair of two-component regulatory genes ecrA1/A2 in S. coelicolor
A1 - LI Yong-quan
A1 - CEN Pei-lin
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A1 - WU Dan
A1 - ZHENG Jing
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Abstract: 
Two-component genes are kinds of genetic elements involved in regulation of antibiotic production in Streptomyces coelicolor. DNA microarray analysis revealed thatecr A1/A2, which mapped at distant sites from red locus and encode respectively the kinase and regulator, expressed coordinately with genes of Red specific biosynthetic pathway. ecrA1 and ecrA2 gene-disruptive mutants were constructed using homogenotisation by reciprocal double crossover. Fermentation data showed that the undecylprodigiosin (Red) level of production was lower than that of wild-type strain. However, the change of the actinorhodin (Act) production level was not significant compared with wild type. Thus, these experiment results confirmed that the two-component system ecrA1/A2 was positive regulatory element for red gene cluster.

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Reference

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