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Journal of Zhejiang University SCIENCE B 2009 Vol.10 No.11 P.820-828


Allograft rejection-related gene expression in the endothelial cells of renal transplantation recipients after cytomegalovirus infection

Author(s):  Yang LI, Hang YAN, Wu-jun XUE, Pu-xun TIAN, Xiao-ming DING, Xiao-ming PAN, Xin-shun FENG, Xiao-hui TIAN, He-li XIANG, Jun HOU

Affiliation(s):  Department of Renal Transplantation, Center of Nephropathy, the First Affiliated Hospital, School of Medicine, Xi’ more

Corresponding email(s):   jullian80915@126.com, yanhang2477@sina.com

Key Words:  Renal transplantation, Cytomegalovirus (CMV), Rejection, Intercellular adhesion molecule-1 (ICAM-1), Major histocompability complex (MHC)

Yang LI, Hang YAN, Wu-jun XUE, Pu-xun TIAN, Xiao-ming DING, Xiao-ming PAN, Xin-shun FENG, Xiao-hui TIAN, He-li XIANG, Jun HOU. Allograft rejection-related gene expression in the endothelial cells of renal transplantation recipients after cytomegalovirus infection[J]. Journal of Zhejiang University Science B, 2009, 10(11): 820-828.

@article{title="Allograft rejection-related gene expression in the endothelial cells of renal transplantation recipients after cytomegalovirus infection",
author="Yang LI, Hang YAN, Wu-jun XUE, Pu-xun TIAN, Xiao-ming DING, Xiao-ming PAN, Xin-shun FENG, Xiao-hui TIAN, He-li XIANG, Jun HOU",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Allograft rejection-related gene expression in the endothelial cells of renal transplantation recipients after cytomegalovirus infection
%A Yang LI
%A Hang YAN
%A Wu-jun XUE
%A Pu-xun TIAN
%A Xiao-ming DING
%A Xiao-ming PAN
%A Xin-shun FENG
%A Xiao-hui TIAN
%A He-li XIANG
%A Jun HOU
%J Journal of Zhejiang University SCIENCE B
%V 10
%N 11
%P 820-828
%@ 1673-1581
%D 2009
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B0920115

T1 - Allograft rejection-related gene expression in the endothelial cells of renal transplantation recipients after cytomegalovirus infection
A1 - Yang LI
A1 - Hang YAN
A1 - Wu-jun XUE
A1 - Pu-xun TIAN
A1 - Xiao-ming DING
A1 - Xiao-ming PAN
A1 - Xin-shun FENG
A1 - Xiao-hui TIAN
A1 - He-li XIANG
A1 - Jun HOU
J0 - Journal of Zhejiang University Science B
VL - 10
IS - 11
SP - 820
EP - 828
%@ 1673-1581
Y1 - 2009
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B0920115

Objective: To explore the effects of cytomegalovirus (CMV) infection on rejection-related gene expression in the endothelial cells of renal transplantation recipients. Methods: Endothelial cells (ECs) were cultured and stimulated by a variety of factors: A, normal control group; B, inactivated human cytomegalovirus (HCMV) infection group; C, HCMV infection group; D, HCMV supernatant infection group; and E, ganciclovir HCMV group. Expression of intercellular adhesion molecule-1 (ICAM-1) and major histocompability complex (MHC) class I and class II antigens was detected by flow cytometry (FCM) and immunohistochemistry. Results: We found characteristic CMV-infected ECs in this study. There were no significant differences among groups A, B and D (P>0.05). Although the expression levels of ICAM-1 were not significantly different between groups C and E (P>0.05), the ICAM-1 expression in these two groups was significantly higher than that in group A (P<0.05). ICAM-1 expression was detected in groups C and E, while there was no expression in groups A, B and D. Furthermore, there was no significant difference of ICAM-1 mRNA expression between groups C and E (P>0.05). Human leucocyte antigen (HLA)-ABC expression was detected in all the groups, while HLA-DR expression was only detected in groups C and E. There were no significant differences of HLA-ABC and HLA-DR expression among groups A, B and D (P>0.05). However, the HLA-ABC and HLA-DR expression levels in groups C and D were higher than those of the remaining groups previously reported (P<0.05). Meanwhile, the HLA-ABC and HLA-DR expression levels in group E were lower than those of group C (P<0.05). Conclusion: CMV could up-regulate the expression levels of ICAM-1 and MHC antigens, which was closely related to allograft rejection.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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