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Received: 2004-03-07

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Journal of Zhejiang University SCIENCE A 2004 Vol.5 No.10 P.1199-1205

http://doi.org/10.1631/jzus.2004.1199


Production of bacterial blight resistant lines from somatic hybridization between Oryza sativa L. and Oryza meyeriana L.


Author(s):  YAN Cheng-qi, QIAN Kai-xian, XUE Gang-ping, WU Zhong-chang, CHEN Yue-lei, YAN Qiu-sheng, ZHANG Xue-qing, Wu Ping

Affiliation(s):  College of Life Sciences, Zhejiang University, Hangzhou 310029, China; more

Corresponding email(s):   Docpwu@zju.edu.cn

Key Words:  Oryza sativa L., Oryza meyeriana L., Somatic hybridization, Rice bacterial blight resistance


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YAN Cheng-qi, QIAN Kai-xian, XUE Gang-ping, WU Zhong-chang, CHEN Yue-lei, YAN Qiu-sheng, ZHANG Xue-qing, Wu Ping. Production of bacterial blight resistant lines from somatic hybridization between Oryza sativa L. and Oryza meyeriana L.[J]. Journal of Zhejiang University Science A, 2004, 5(10): 1199-1205.

@article{title="Production of bacterial blight resistant lines from somatic hybridization between Oryza sativa L. and Oryza meyeriana L.",
author="YAN Cheng-qi, QIAN Kai-xian, XUE Gang-ping, WU Zhong-chang, CHEN Yue-lei, YAN Qiu-sheng, ZHANG Xue-qing, Wu Ping",
journal="Journal of Zhejiang University Science A",
volume="5",
number="10",
pages="1199-1205",
year="2004",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.2004.1199"
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%T Production of bacterial blight resistant lines from somatic hybridization between Oryza sativa L. and Oryza meyeriana L.
%A YAN Cheng-qi
%A QIAN Kai-xian
%A XUE Gang-ping
%A WU Zhong-chang
%A CHEN Yue-lei
%A YAN Qiu-sheng
%A ZHANG Xue-qing
%A Wu Ping
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%P 1199-1205
%@ 1869-1951
%D 2004
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.2004.1199

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T1 - Production of bacterial blight resistant lines from somatic hybridization between Oryza sativa L. and Oryza meyeriana L.
A1 - YAN Cheng-qi
A1 - QIAN Kai-xian
A1 - XUE Gang-ping
A1 - WU Zhong-chang
A1 - CHEN Yue-lei
A1 - YAN Qiu-sheng
A1 - ZHANG Xue-qing
A1 - Wu Ping
J0 - Journal of Zhejiang University Science A
VL - 5
IS - 10
SP - 1199
EP - 1205
%@ 1869-1951
Y1 - 2004
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.2004.1199


Abstract: 
Novel bacterial blight (BB) resistance gene(s) for rice was (were) introduced into a cultivated japonica rice variety Oryza sativa (cv. 8411), via somatic hybridization using the wild rice Oryza meyeriana as the donor of the resistance gene(s). Twenty-nine progenies of somatically hybridized plants were obtained. Seven somatically hybridized plants and their parents were used for AFLP (amplified fragment length polymorphism) analysis using 8 primer pairs. Results confirmed that these plants were somatic hybrids containing the characteristic bands of both parents. The morphology of the regenerated rice showed characters of both O. sativa and O. meyeriana. Two somatic hybrids showed highest BB resistance and the other 8 plants showed moderate resistance. The new germplasms with highest resistance have been used in the rice breeding program for the improvement of bacterial blight resistance.

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Reference

[1] Abdullah, R., Cocking, E.C., Thompsom, A., 1986. Efficient plant regeneration from rice protoplasts through somatic embryogenesis. Bio/Technology, 4:1087-1090.

[2] Akagi, H., Sakamoto, M., Negishi, T., Fujimura, T., 1989. Construction of rice hybrid plants. Mol. Gen. Genet., 215:501-506.

[3] Frearson, E.M., Power, J.B., Cocking, E.C., 1973. The isolation culture and regeneration of Petunia leaf protoplasts. Dev. Biol., l33:130-137.

[4] Hayashi, Y., Kyozuka, J., Shimamoto, K., 1988. Hybrids of rice (Oryza sativa L) and wild Oryza species obtained by cell fusion. Mol. Gen. Genet., 214:6-10.

[5] Jena, K.K., Khush, G.S., 1990. Introgression of genes from Oryza officinalis well to cultivated Oryza sativa L. Theor. Appl. Genet., 80:737-745.

[6] Jelodar, N.B., Blackhall, N.W., Hartman, T.P.V., Brar, D.S., Khush, G., Davey, M.R., Cocking, E.C., 1999. Intergeneic somatic hybrids of rice (Oryza sativa L.) and Porteresia coarctata (Roxb.) Tateok. Theor. Appl. Genet., 99:570-577.

[7] Kao, K.N., 1997. Chromosomal behaviour in somatic hybrids of soybean-Nicotiana glauca. Mol. Gen. Genet., 150:225-230.

[8] Kauffman, H.E., Reddy, A.P.K.S., Hsieh, P.Y., Nerca, S.D., 1973. An improved technique for evalution of resistance of rice varieties to Xanthomonas Oryzae. Plant Dis Rep., 57:537-541.

[9] Kisaka, H., Kisaka, M., Kanno, A., Kameya, T., 1998. Intergeneric somatic hybridization of rice (Oryza sativa L) and barley (Hordeum vulgare L) by protoplast fusion. Plant Cell Rep., 17:362-367.

[10] Levan, A., Fredya, K., Sandberg, A., 1964. Nomenclature for centromeric position on chromosome. Hereditas, 54:201-220.

[11] Linsmaier, E.M., Skoog, F., 1965. Organic growth factor requirements of tobacco tissue cultures. Physiologia Plantarum, 18:100-127.

[12] Peng, S.Q., Liu, E.M., Huang, F.Y., Xiao, F.G., Fan, K.C., Luo, L.M., Chen, Y., 1996. Research on durable resistance to rice blast disease. Acta Phytophylacica Sinina, 23:293-299 (in Chinese).

[13] Porebski, S., Bailey, G., Baum, B.R., 1997. Modification of CTAB DNA extraction protocol for plants containing high polysaccharide and polyphenol components. Plant Mol. Biol. Rep., 15:8-15.

[14] Vos, P., Hogers, R., Bleeker, M., Reijans, M., Van De Lee, T., Hornes, M., Frijters, A., Pot, J., Peleman, J., Kuiper, M., Zabeau, M., 1995. AFLP: A new technique for DNA fingerprinting. Nucleic Acids Res., 23:4407-4414.

[15] Yan, C.Q., Yan, Q.S., Qian, K.X., Xue, G.P., Wu, P., 2002. Plant Regeneration from Somatic Hybridization between Oryza Meyerana and O. Sativa cv. 8411. International Rice Congress Abstract, p.267-268.

[16] Yang, Z.Q., Shiknai, T., Yamada, Y., 1988. Asymmetric fertile rice (Oryza sativa L.) protoplast. Theor. Appl. Genet., 76:801-808.

[17] Zhang, X.Q., Yan, Q.S., Teng, S., 1999. Study on transfer of apomixes gene of Panicum maximum into rice plant. Chinese Science, 5:1579-1580 (in Chinese).

[18] Zhu, Z.Q., Wang, J.J., Shen, J.S., Xu, Z., Zhu, Z.Y., Yang, G.C., Bei, F.Y., 1975. Establish rice anther culture medium by nitrogen content. Sci China (B), 5:484-490 (in Chinese).

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