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Journal of Zhejiang University SCIENCE B 2005 Vol.6 No.1 P.14-21

http://doi.org/10.1631/jzus.2005.B0014


Intestinal microflora in rats with ischemia/reperfusion liver injury


Author(s):  XING Hui-chun, LI Lan-juan, XU Kai-jin, SHEN Tian, CHEN Yun-bo, SHENG Ji-fang, YU Yun-song, CHEN Ya-gang

Affiliation(s):  Key Laboratory of Infectious Disease, Ministry of Public Health of China, Institute of Infectious Diseases, Department of Infectious Diseases, First Affiliated Hospital, Schhool of Medicine, Zhejiang University, Hangzhou 310003, China

Corresponding email(s):   ljli@zjwst.gov.cn

Key Words:  Ischemia/reperfusion (I/R), Liver injury, Microflora, Endotoxin, Bacterial translocation


XING Hui-chun, LI Lan-juan, XU Kai-jin, SHEN Tian, CHEN Yun-bo, SHENG Ji-fang, YU Yun-song, CHEN Ya-gang. Intestinal microflora in rats with ischemia/reperfusion liver injury[J]. Journal of Zhejiang University Science B, 2005, 6(1): 14-21.

@article{title="Intestinal microflora in rats with ischemia/reperfusion liver injury",
author="XING Hui-chun, LI Lan-juan, XU Kai-jin, SHEN Tian, CHEN Yun-bo, SHENG Ji-fang, YU Yun-song, CHEN Ya-gang",
journal="Journal of Zhejiang University Science B",
volume="6",
number="1",
pages="14-21",
year="2005",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.2005.B0014"
}

%0 Journal Article
%T Intestinal microflora in rats with ischemia/reperfusion liver injury
%A XING Hui-chun
%A LI Lan-juan
%A XU Kai-jin
%A SHEN Tian
%A CHEN Yun-bo
%A SHENG Ji-fang
%A YU Yun-song
%A CHEN Ya-gang
%J Journal of Zhejiang University SCIENCE B
%V 6
%N 1
%P 14-21
%@ 1673-1581
%D 2005
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.2005.B0014

TY - JOUR
T1 - Intestinal microflora in rats with ischemia/reperfusion liver injury
A1 - XING Hui-chun
A1 - LI Lan-juan
A1 - XU Kai-jin
A1 - SHEN Tian
A1 - CHEN Yun-bo
A1 - SHENG Ji-fang
A1 - YU Yun-song
A1 - CHEN Ya-gang
J0 - Journal of Zhejiang University Science B
VL - 6
IS - 1
SP - 14
EP - 21
%@ 1673-1581
Y1 - 2005
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.2005.B0014


Abstract: 
Objectives: To investigate the intestinal microflora status related to ischemia/reperfusion (I/R) liver injury and explore the possible mechanism. Methods: Specific pathogen free grade Sprague-Dawley rats were randomized into three groups: Control group (n=8), sham group (n=6) and I/R group (n=10). Rats in the control group did not receive any treatment, rats in the I/R group were subjected to 20 min of liver ischemia, and rats in the sham group were only subjected to sham operation. Twenty-two hours later, the rats were sacrificed and liver enzymes and malondialdehyde (MDA), superoxide dismutase (SOD), serum endotoxin, intestinal bacterial count, intestinal mucosal histology, bacterial translocation to mesenteric lymph nodes, liver, spleen, and kidney were studied. Results: Ischemia/reperfusion increased liver enzymes, MDA, decreased SOD, and was associated with plasma endotoxin elevation in I/R group campared to those in the sham group. Intestinal Bifidobacterium and Lactobacillus decreased and intestinal Enterobacteria and Enterococci, bacterial translocation to kidney increased in the I/R group compared to the sham group. Intestinal microvilli were lost, disrupted and the interspace between cells became wider in the I/R group. Conclusion: I/R liver injury may lead to disturbance of intestinal microflora and impairment of intestinal mucosal barrier function, which contributes to endotoxemia and bacterial translocation to kidney.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

Reference

[1] Albillos, A., De la Hera, A., 2002. Multifactorial gut barrier failure in cirrhosis and bacterial translocation: working out the role of probiotics and antioxidants. J Hepatol, 37:523-526.

[2] Amarri, S., Masetti, M., Benatti, F., Callegari, M.L., Morelli, L., Villa, E., Balli, F., Jovine, E., Pinna, A.D., 2002. Small intestine microflora after intestinal/multivisceral transplantation: preliminary results. Transplantat Proc, 34:953-954.

[3] Armeni, T., Ghiselli, R., Balercia, G., Goffi, L., Jassem, W., Saba, V., Principato, G., 2000. Glutathione and ultrastructural changes in inflow occlusion of rat liver. J Surg Research, 88:207-221.

[4] Caban, A., Oczkowicz, G., Abdel-Samad, O., Cierpka, L., 2002. Influence of Kupffer cells on the early phase of liver reperfusion. Transplant Proc, 34(2):694-697.

[5] Chan, C.S., Chen, G.H., Lien, H.C., Yeh, H.Z., 1998. Small intestine dismotility and bacterial overgrowth in cirrhotic patients with spontane bacterial peritonitis. Hepatology, 28:1187-1190.

[6] Chiva, M., Soriano, G., Rochat, I., Peralta, C., Rochat, F., Llovet, T., Mirelis, B., Schiffrin, E.J., Guarner, C., Balanzo, J., 2002. Effect of Lactobacillus johnsonii La1 and antioxidants on intestinal flora and bacterial translocation in rats with experimental cirrhosis. J Hepatol, 37:456-462.

[7] Colak, T., Ozturk, C., Polat, A., Bagdatoglu, O., Kanik, A., Turkmenoglu, O., Aydin, S., 2003. Effects of trapidil on intestinal mucosal barrier function and bacterial translocation after intestinal ischemia and reperfusion in an experimental rat model. Current Therapeutic Research, 64(6):355-366.

[8] Cutrín, J.C., Perrelli, M.G., Cavalieri, B., Peralta, C., Catafau, J.R., Poli, G., 2002. Microvascular dysfunction induced by reperfusion injury and protective effect of ischemic preconditioning. Free Radical Biology & Medicine, 33(9):1200-1208.

[9] Hartemink, R., Domenech, V.R., Rombouts, F.M.L., 1997. AMVABa new selective medium for the isolation of laxtobacilli from faeces. J Microbio Methods, 29:77-84.

[10] Kasravi, F.B., Adawi, D., Molin, G., Bengmark, S., Jeppsson, B., 1997. Effect of oral supplementation of lactobacilli on bacterial translocation in acute liver injury induced by d-galactosamine. J Hepatol, 26:417-424.

[11] Kukan, M., Bezek, S., Trnovec, T., 1996. Role of hepatovasculature in warm ischaemia-reperfusion injury of rat liver. Physiol Res, 45(5):427-430.

[12] Li, X.T., Qiu, H., 1998. Lecture on Mitsuoka’s method for analysing intestinal microorganism and metabolite. Chin J Microecology, 10:61-63 (in Chinese).

[13] Li, L.J., Wu, Z.W., Xiao, D.S., Sheng, J.F., 2004. Changes of gut flora and endotoxin in rats with D-galactosamine-induced acute liver failure. World J Gastroenterol, 10(14):2087-2090.

[14] Ren, D., Han, D., Zhao, Y., 2001. Effect of intestinal endotoxemia on hepatic energy metabolism in acute liver failure. Chin J Pathophy, 17(9):890-892 (in Chinese).

[15] Serracino-Inglott, F., Habib, N.A., Mathie, R.T., 2001. Hepatic ischemia-reperfusion injury. Am J Surg, 181:160-166.

[16] Serrano, E., Diaz, J., Acosta, F., Palenciano, C.G., Parrilla, P., Carbonell, L.F., 2000. Oxidative stress during ischemia-reperfusion in liver transplantation. Transplant Proc, 32:2651-2651.

[17] Tuohy, K.M., Probert, H.M., Smejkal, C.W., Gibson, G.R., 2003. Using probiotics and prebiotics to improve gut health. DDT, 8(15):692-700.

[18] Vajdova, K., Smrekova, R., Kukan, M., Jakubovsky, J., Rooijen, N., Horecky, J., Lutterova, M., Wsolova, L., 2000. Endotoxin-induced aggravation of preservation-reperfusion injury of rat liver and its modulation. J Hepatol, 32(1):112-120.

[19] Wang, Z., Xiao, G., Yao, Y., Wang, H., Sheng, Z., Cai, B., Xiao, J., 2002. The relationship between intestinal bifidobacteria and bacteria/endotoxin translocation in scalded rats. Chin J Burns, 18(6):365-368 (in Chinese).

[20] Wells, C.L., Maddaus, M.A., Reynolds, C.M., Jechorek, R.P., Simmons, R.L., 1987. Role of anaerobic flora in the translocation of aerobic and facultatively anaerobic intestinal bacteria. Infect Immun, 55:2689-2694.

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