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Journal of Zhejiang University SCIENCE B 2005 Vol.6 No.1 P.14-21


Intestinal microflora in rats with ischemia/reperfusion liver injury

Author(s):  XING Hui-chun, LI Lan-juan, XU Kai-jin, SHEN Tian, CHEN Yun-bo, SHENG Ji-fang, YU Yun-song, CHEN Ya-gang

Affiliation(s):  Key Laboratory of Infectious Disease, Ministry of Public Health of China, Institute of Infectious Diseases, Department of Infectious Diseases, First Affiliated Hospital, Schhool of Medicine, Zhejiang University, Hangzhou 310003, China

Corresponding email(s):   ljli@zjwst.gov.cn

Key Words:  Ischemia/reperfusion (I/R), Liver injury, Microflora, Endotoxin, Bacterial translocation

XING Hui-chun, LI Lan-juan, XU Kai-jin, SHEN Tian, CHEN Yun-bo, SHENG Ji-fang, YU Yun-song, CHEN Ya-gang. Intestinal microflora in rats with ischemia/reperfusion liver injury[J]. Journal of Zhejiang University Science B, 2005, 6(1): 14-21.

@article{title="Intestinal microflora in rats with ischemia/reperfusion liver injury",
author="XING Hui-chun, LI Lan-juan, XU Kai-jin, SHEN Tian, CHEN Yun-bo, SHENG Ji-fang, YU Yun-song, CHEN Ya-gang",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Intestinal microflora in rats with ischemia/reperfusion liver injury
%A XING Hui-chun
%A LI Lan-juan
%A XU Kai-jin
%A SHEN Tian
%A CHEN Yun-bo
%A SHENG Ji-fang
%A YU Yun-song
%A CHEN Ya-gang
%J Journal of Zhejiang University SCIENCE B
%V 6
%N 1
%P 14-21
%@ 1673-1581
%D 2005
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.2005.B0014

T1 - Intestinal microflora in rats with ischemia/reperfusion liver injury
A1 - XING Hui-chun
A1 - LI Lan-juan
A1 - XU Kai-jin
A1 - SHEN Tian
A1 - CHEN Yun-bo
A1 - SHENG Ji-fang
A1 - YU Yun-song
A1 - CHEN Ya-gang
J0 - Journal of Zhejiang University Science B
VL - 6
IS - 1
SP - 14
EP - 21
%@ 1673-1581
Y1 - 2005
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.2005.B0014

Objectives: To investigate the intestinal microflora status related to ischemia/reperfusion (I/R) liver injury and explore the possible mechanism. Methods: Specific pathogen free grade Sprague-Dawley rats were randomized into three groups: Control group (n=8), sham group (n=6) and I/R group (n=10). Rats in the control group did not receive any treatment, rats in the I/R group were subjected to 20 min of liver ischemia, and rats in the sham group were only subjected to sham operation. Twenty-two hours later, the rats were sacrificed and liver enzymes and malondialdehyde (MDA), superoxide dismutase (SOD), serum endotoxin, intestinal bacterial count, intestinal mucosal histology, bacterial translocation to mesenteric lymph nodes, liver, spleen, and kidney were studied. Results: Ischemia/reperfusion increased liver enzymes, MDA, decreased SOD, and was associated with plasma endotoxin elevation in I/R group campared to those in the sham group. Intestinal Bifidobacterium and Lactobacillus decreased and intestinal Enterobacteria and Enterococci, bacterial translocation to kidney increased in the I/R group compared to the sham group. Intestinal microvilli were lost, disrupted and the interspace between cells became wider in the I/R group. Conclusion: I/R liver injury may lead to disturbance of intestinal microflora and impairment of intestinal mucosal barrier function, which contributes to endotoxemia and bacterial translocation to kidney.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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