CLC number: R730.1; R730.231.3
On-line Access: 2024-08-27
Received: 2023-10-17
Revision Accepted: 2024-05-08
Crosschecked: 0000-00-00
Cited: 11
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CHEN Zhe, XU Ze-feng, YE Jing-jia, YAO Hang-ping, ZHENG Shu, DING Jia-yi. Combination of small interfering RNAs mediates greater inhibition of human hepatitis B virus replication and antigen expression[J]. Journal of Zhejiang University Science B, 2005, 6(4): 236-241.
@article{title="Combination of small interfering RNAs mediates greater inhibition of human hepatitis B virus replication and antigen expression",
author="CHEN Zhe, XU Ze-feng, YE Jing-jia, YAO Hang-ping, ZHENG Shu, DING Jia-yi",
journal="Journal of Zhejiang University Science B",
volume="6",
number="4",
pages="236-241",
year="2005",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.2005.B0236"
}
%0 Journal Article
%T Combination of small interfering RNAs mediates greater inhibition of human hepatitis B virus replication and antigen expression
%A CHEN Zhe
%A XU Ze-feng
%A YE Jing-jia
%A YAO Hang-ping
%A ZHENG Shu
%A DING Jia-yi
%J Journal of Zhejiang University SCIENCE B
%V 6
%N 4
%P 236-241
%@ 1673-1581
%D 2005
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.2005.B0236
TY - JOUR
T1 - Combination of small interfering RNAs mediates greater inhibition of human hepatitis B virus replication and antigen expression
A1 - CHEN Zhe
A1 - XU Ze-feng
A1 - YE Jing-jia
A1 - YAO Hang-ping
A1 - ZHENG Shu
A1 - DING Jia-yi
J0 - Journal of Zhejiang University Science B
VL - 6
IS - 4
SP - 236
EP - 241
%@ 1673-1581
Y1 - 2005
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.2005.B0236
Abstract: Objectives: To evaluate the inhibitory effect mediated by combination of small interfering RNAs (siRNAs) targeting different sites of hepatitis B virus (HBV) transcripts on the viral replication and antigen expression in vitro. Methods: (1) Seven siRNAs targeting surface (S), polymerase (P) or precore (PreC) region of HBV genome were designed and chemically synthesized. (2) HBV-producing HepG2.2.15 cells were treated with or without siRNAs for 72 h. (3) HBsAg and HBeAg in the cell culture medium were detected by enzyme-linked immunoadsorbent assay. (4) Intracellular viral DNA was quantified by real-time PCR (Polymerase Chain Reaction). (5) HBV viral mRNA was reverse transcribed and quantified by real-time PCR. (6) The change of cell cycle and apoptosis was determined by flow cytometry. Results: Our data demonstrated that synthetic small interfering RNAs (siRNAs) targeting S and PreC gene could efficiently and specifically inhibit HBV replication and antigen expression. The expression of HBsAg and HBeAg and the replication of HBV could be specifically inhibited in a dose-dependent manner by siRNAs. Furthermore, our results showed that the combination of siRNAs targeting various regions could inhibit HBV replication and antigen expression in a more efficient way than the use of single siRNA at the same final concentration. No apoptotic change was observed in the cell after siRNA treatment. Conclusion: Our results demonstrated that siRNAs exerted robust and specific inhibition on HBV replication and antigen expression in a cell culture system and combination of siRNAs targeting different regions exhibited more potency.
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