CLC number: R575
On-line Access:
Received: 1999-08-18
Revision Accepted: 2000-07-05
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CHEN Zhi, LIU Yong, LIU Ke-zhou, Dennin R.H, Reinhard U, Dou Jun. IN VITRO STUDY ON RIBOZYME AGAINST HEPATITIS C VIRUS[J]. Journal of Zhejiang University Science A, 2001, 2(1): 100-103.
@article{title="IN VITRO STUDY ON RIBOZYME AGAINST HEPATITIS C VIRUS",
author="CHEN Zhi, LIU Yong, LIU Ke-zhou, Dennin R.H, Reinhard U, Dou Jun",
journal="Journal of Zhejiang University Science A",
volume="2",
number="1",
pages="100-103",
year="2001",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.2001.0100"
}
%0 Journal Article
%T IN VITRO STUDY ON RIBOZYME AGAINST HEPATITIS C VIRUS
%A CHEN Zhi
%A LIU Yong
%A LIU Ke-zhou
%A Dennin R.H
%A Reinhard U
%A Dou Jun
%J Journal of Zhejiang University SCIENCE A
%V 2
%N 1
%P 100-103
%@ 1869-1951
%D 2001
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.2001.0100
TY - JOUR
T1 - IN VITRO STUDY ON RIBOZYME AGAINST HEPATITIS C VIRUS
A1 - CHEN Zhi
A1 - LIU Yong
A1 - LIU Ke-zhou
A1 - Dennin R.H
A1 - Reinhard U
A1 - Dou Jun
J0 - Journal of Zhejiang University Science A
VL - 2
IS - 1
SP - 100
EP - 103
%@ 1869-1951
Y1 - 2001
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.2001.0100
Abstract: Objective: To determine the effective nucleotide sites of ribozymes against HCV, and obtain a highly effective, nontoxic and inexpensive antisense ribozyme specific for HCV. Methods: Two effective ribozymes, targeted to HCV 5'NC region and C region, were designed and synthesized.Eukaryotic expression vectors, pSV2-gpt. CD-SR, containing either HCRZNC or HCRZC were constructed and transfected into MT-2 cells, which had been infected by HCV. Quantitative PCR and hybridization were used to determine the effect of inhibition of HCV by ribozymes. Results: HCRZNC and HCRZC suppressed the replication of HCV by 54.7% and 2.1%, respectively. Furthermore, when both ribozymes were cotransfected into cells, they suppressed replication by 78.8%. Conclusion: Two specific antisense ribozymes have strong inhibitory effects on the replication of HCV in cultured cells, and have better effect when used together.
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