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Journal of Zhejiang University SCIENCE A 2001 Vol.2 No.1 P.100-103

http://doi.org/10.1631/jzus.2001.0100


IN VITRO STUDY ON RIBOZYME AGAINST HEPATITIS C VIRUS


Author(s):  CHEN Zhi, LIU Yong, LIU Ke-zhou, Dennin R.H, Reinhard U, Dou Jun

Affiliation(s):  Institute of Infectious Diseases, Medical College, Zhejiang University, Hangzhou 310003, China; more

Corresponding email(s): 

Key Words:  ribozyme, inhibition, hepatitis C virus, MT-2 cells


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CHEN Zhi, LIU Yong, LIU Ke-zhou, Dennin R.H, Reinhard U, Dou Jun. IN VITRO STUDY ON RIBOZYME AGAINST HEPATITIS C VIRUS[J]. Journal of Zhejiang University Science A, 2001, 2(1): 100-103.

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author="CHEN Zhi, LIU Yong, LIU Ke-zhou, Dennin R.H, Reinhard U, Dou Jun",
journal="Journal of Zhejiang University Science A",
volume="2",
number="1",
pages="100-103",
year="2001",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.2001.0100"
}

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%A CHEN Zhi
%A LIU Yong
%A LIU Ke-zhou
%A Dennin R.H
%A Reinhard U
%A Dou Jun
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%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.2001.0100

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A1 - CHEN Zhi
A1 - LIU Yong
A1 - LIU Ke-zhou
A1 - Dennin R.H
A1 - Reinhard U
A1 - Dou Jun
J0 - Journal of Zhejiang University Science A
VL - 2
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%@ 1869-1951
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Abstract: 
Objective: To determine the effective nucleotide sites of ribozymes against HCV, and obtain a highly effective, nontoxic and inexpensive antisense ribozyme specific for HCV. Methods: Two effective ribozymes, targeted to HCV 5'NC region and C region, were designed and synthesized.Eukaryotic expression vectors, pSV2-gpt. CD-SR, containing either HCRZNC or HCRZC were constructed and transfected into MT-2 cells, which had been infected by HCV. Quantitative PCR and hybridization were used to determine the effect of inhibition of HCV by ribozymes. Results: HCRZNC and HCRZC suppressed the replication of HCV by 54.7% and 2.1%, respectively. Furthermore, when both ribozymes were cotransfected into cells, they suppressed replication by 78.8%. Conclusion: Two specific antisense ribozymes have strong inhibitory effects on the replication of HCV in cultured cells, and have better effect when used together.

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Reference

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