Full Text:   <2075>

CLC number: R575

On-line Access: 

Received: 1999-08-18

Revision Accepted: 2000-07-05

Crosschecked: 0000-00-00

Cited: 0

Clicked: 4355

Citations:  Bibtex RefMan EndNote GB/T7714

-   Go to

Article info.
1. Reference List
Open peer comments

Journal of Zhejiang University SCIENCE A 2001 Vol.2 No.1 P.100-103

http://doi.org/10.1631/jzus.2001.0100


IN VITRO STUDY ON RIBOZYME AGAINST HEPATITIS C VIRUS


Author(s):  CHEN Zhi, LIU Yong, LIU Ke-zhou, Dennin R.H, Reinhard U, Dou Jun

Affiliation(s):  Institute of Infectious Diseases, Medical College, Zhejiang University, Hangzhou 310003, China; more

Corresponding email(s): 

Key Words:  ribozyme, inhibition, hepatitis C virus, MT-2 cells


Share this article to: More

CHEN Zhi, LIU Yong, LIU Ke-zhou, Dennin R.H, Reinhard U, Dou Jun. IN VITRO STUDY ON RIBOZYME AGAINST HEPATITIS C VIRUS[J]. Journal of Zhejiang University Science A, 2001, 2(1): 100-103.

@article{title="IN VITRO STUDY ON RIBOZYME AGAINST HEPATITIS C VIRUS",
author="CHEN Zhi, LIU Yong, LIU Ke-zhou, Dennin R.H, Reinhard U, Dou Jun",
journal="Journal of Zhejiang University Science A",
volume="2",
number="1",
pages="100-103",
year="2001",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.2001.0100"
}

%0 Journal Article
%T IN VITRO STUDY ON RIBOZYME AGAINST HEPATITIS C VIRUS
%A CHEN Zhi
%A LIU Yong
%A LIU Ke-zhou
%A Dennin R.H
%A Reinhard U
%A Dou Jun
%J Journal of Zhejiang University SCIENCE A
%V 2
%N 1
%P 100-103
%@ 1869-1951
%D 2001
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.2001.0100

TY - JOUR
T1 - IN VITRO STUDY ON RIBOZYME AGAINST HEPATITIS C VIRUS
A1 - CHEN Zhi
A1 - LIU Yong
A1 - LIU Ke-zhou
A1 - Dennin R.H
A1 - Reinhard U
A1 - Dou Jun
J0 - Journal of Zhejiang University Science A
VL - 2
IS - 1
SP - 100
EP - 103
%@ 1869-1951
Y1 - 2001
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.2001.0100


Abstract: 
Objective: To determine the effective nucleotide sites of ribozymes against HCV, and obtain a highly effective, nontoxic and inexpensive antisense ribozyme specific for HCV. Methods: Two effective ribozymes, targeted to HCV 5'NC region and C region, were designed and synthesized.Eukaryotic expression vectors, pSV2-gpt. CD-SR, containing either HCRZNC or HCRZC were constructed and transfected into MT-2 cells, which had been infected by HCV. Quantitative PCR and hybridization were used to determine the effect of inhibition of HCV by ribozymes. Results: HCRZNC and HCRZC suppressed the replication of HCV by 54.7% and 2.1%, respectively. Furthermore, when both ribozymes were cotransfected into cells, they suppressed replication by 78.8%. Conclusion: Two specific antisense ribozymes have strong inhibitory effects on the replication of HCV in cultured cells, and have better effect when used together.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

Reference

[1] Chen,Z,Dennin R.H.,He,N.X.et al.,1995.Detection of minus strand RNA of HCV in liver tissues of patient with hepatitis C and hepatocellular carcinoma by RT-PCR.Chinese J.of Microbiology and Immunology,15(2): 116-118 (in Chinese,with English abstracct).

[2] Hagiwara,H.,Hangashi,N.,Mitta,E.et al.,1993.Quantitative analysis of hepatitis C virus RNA in serum during interferon therapy.Gaseroenterology,104: 877.

[3] Haseloff,J.,Gerlach,W.L.,1988.Simple RNA enzymes with new and highly specific endoribonuclease activities.Nature.18,334(6183):585.

[4] Kasahara,A.,Hagiwara,H.,Hayasi,N.,et al.,1995.Ability of prolonged interferon treatment to suppress ralapse after cessation of therapy in patients with chronic hepatitis C a multicenter randomized controlled trial. Hepatology,21:291-297.

[5] Kruger,K,Grabowski,P.J,Zaug A.J.et al.,1982.Self-splicing RNA: autoexcision and autocyclization of the ribosomal RNA intervening sequence of tetrahymena.Cell.31(1):147.

[6] Mitta,E.,Hayashi,N.,Hagiwara H,et al.,1994.predicting interferon therapy efficacy from hepatitis C virus genometype and RNA titer.Dig.Dis Sci; 39:977

[7] Ohkawa,J.,Koguma,T.,Kohda,T.,et al.,1995.Ribozymes: from mechanistic studies to applications in vivo.J Biochem (Tokyo).118(2):251.

[8] Sakamoto,N.,Wu,C.H.,Wu,G.Y.,1996.Intracellular cleavage of hepatitis C virus RNA and inhibition of viral protein translation by hammerhead ribozymes.J Clin Invest.98(12):2720.

[9] Sarver,N.,Cantin,E.M.,Chang P.S.et al.,1990.Ribozymes as potential anti-HIV-1 therapeutic agents.Science.247(4947):1222.

[10] Weizsacker I.F.,Blum H.E.,Wards G.R.,1992.Cleavage of hepatitis B virus RNA by three ribozymes transcribed from a single DNA template.Biochem Biophys Res Commun.189: 243.

[11] Welch,P.J.,Tritz,R.,Yei.S.,et al.,1996.A Polential therapeutic application of hairpin ribozymes: in vitro and in vivo studies of gene therapy for hapatitis C virus infeetion.Gene ther.3(11): 994-1001.

Open peer comments: Debate/Discuss/Question/Opinion

<1>

Please provide your name, email address and a comment





Journal of Zhejiang University-SCIENCE, 38 Zheda Road, Hangzhou 310027, China
Tel: +86-571-87952783; E-mail: cjzhang@zju.edu.cn
Copyright © 2000 - 2022 Journal of Zhejiang University-SCIENCE