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Journal of Zhejiang University SCIENCE B 2005 Vol.6 No.6 P.477-482


Identification of Rhodiola species by using RP-HPLC

Author(s):  WANG Qiang, RUAN Xiao, JIN Zhi-hua, YAN Qi-chuan, TU Shanjun

Affiliation(s):  Ningbo Institute of Technology, Zhejiang University, Ningbo 315100, China; more

Corresponding email(s):   wangqiangsky@263.net

Key Words:  Rhodiola, Genetic diversity, Species identification, RP-HPLC

WANG Qiang, RUAN Xiao, JIN Zhi-hua, YAN Qi-chuan, TU Shanjun. Identification of Rhodiola species by using RP-HPLC[J]. Journal of Zhejiang University Science B, 2005, 6(6): 477-482.

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author="WANG Qiang, RUAN Xiao, JIN Zhi-hua, YAN Qi-chuan, TU Shanjun",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Identification of Rhodiola species by using RP-HPLC
%A WANG Qiang
%A RUAN Xiao
%A JIN Zhi-hua
%A YAN Qi-chuan
%A TU Shanjun
%J Journal of Zhejiang University SCIENCE B
%V 6
%N 6
%P 477-482
%@ 1673-1581
%D 2005
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.2005.B0477

T1 - Identification of Rhodiola species by using RP-HPLC
A1 - WANG Qiang
A1 - RUAN Xiao
A1 - JIN Zhi-hua
A1 - YAN Qi-chuan
A1 - TU Shanjun
J0 - Journal of Zhejiang University Science B
VL - 6
IS - 6
SP - 477
EP - 482
%@ 1673-1581
Y1 - 2005
PB - Zhejiang University Press & Springer
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DOI - 10.1631/jzus.2005.B0477

An approach was established using RP-HPLC (reversed-phase high-performance liquid chromatography) to identify ten species of Rhodiola, R. coccinea A. Bor, R. junggarica C.Y. Yang et N.R. Cui spn., R. heterodonta A. Bor, R. linearifolia A. Bor, R. pamiro alaiucm A. Bor, R. kaschgarica A. Bor, R. litwinowii A. Bor, R. gelida schrenk, R. rosea L. and R. quadrifide Fisch et Mey collected from the Tianshan Mountains areas of China. Chromatograms of alcohol-soluble proteins, generated from these ten Rhodiola spp. were compared. Each chromatogram of alcohol-soluble proteins came from a single seed of one wild species only. The results showed that when using a Waters Delta Pak. C18, 5 μm particle size reversed phase column (150 mm×3.9 mm), a linear gradient of 22%−55% solvent B with a flow rate of 1 ml/min and a run time of 67 min, the chromatography gave optimum separation of Rhodiola alcohol-soluble proteins. Chromatogram of each species was different and could be used to identify those species. Cluster analysis of genetic similarity coefficients of 37% to 60% showed a medium degree of genetic diversity among the species in these eco-areas. Cluster analysis showed that the ten species of Rhodiola can be divided into four clusters and yielded the general and unique biochemical markers of these species. RP-HPLC was shown to be a rapid, repeatable and reliable method for Rhodiola species identification and analysis of genetic diversity.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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