CLC number: TP23
On-line Access: 2024-08-27
Received: 2023-10-17
Revision Accepted: 2024-05-08
Crosschecked: 0000-00-00
Cited: 0
Clicked: 6120
YE Shu-ming, GAO Shao-jia, PAN Yi, YANG Jun-yi, LI Shi-lun. Automatic separation system for marine meiobenthos based on laser-induced fluorescence technology[J]. Journal of Zhejiang University Science B, 2005, 6(6): 535-539.
@article{title="Automatic separation system for marine meiobenthos based on laser-induced fluorescence technology",
author="YE Shu-ming, GAO Shao-jia, PAN Yi, YANG Jun-yi, LI Shi-lun",
journal="Journal of Zhejiang University Science B",
volume="6",
number="6",
pages="535-539",
year="2005",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.2005.B0535"
}
%0 Journal Article
%T Automatic separation system for marine meiobenthos based on laser-induced fluorescence technology
%A YE Shu-ming
%A GAO Shao-jia
%A PAN Yi
%A YANG Jun-yi
%A LI Shi-lun
%J Journal of Zhejiang University SCIENCE B
%V 6
%N 6
%P 535-539
%@ 1673-1581
%D 2005
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.2005.B0535
TY - JOUR
T1 - Automatic separation system for marine meiobenthos based on laser-induced fluorescence technology
A1 - YE Shu-ming
A1 - GAO Shao-jia
A1 - PAN Yi
A1 - YANG Jun-yi
A1 - LI Shi-lun
J0 - Journal of Zhejiang University Science B
VL - 6
IS - 6
SP - 535
EP - 539
%@ 1673-1581
Y1 - 2005
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.2005.B0535
Abstract: An automatic system for marine meiobenthos separation was developed by using laser-induced fluorescence technology. rose Bengal was used as organism dye and the spectrums of rose Bengal were measured. Laser-induced fluorescence system was established to detect marine meiobenthos in sediments. Data obtained from experiments were analyzed by using a mathematical model. The results showed that laser-induced fluorescence technology worked well in the system. The system could select the meiobenthos efficiently and precisely.
[1] Chuck, R.S., Arnoldussen, M.E., Behrens, A., 2002. Fluorescence-guided laser removal of chemically damaged cornea. Journal of Cataract & Refractive Surgery, 28(10):1847-1852.
[2] De Jonge, V.N., Bouwman, L.A., 1977. A simple density separation technique for quantitative isolation of meiobenthos using the colloidal silica Ludox-TM. Marine Biology, 42(2):143-148.
[3] Dillon, W.P., 1964. Flotation technique for separating fecal pellets and small marine organisms from sand. Limnology and oceanography, 9:601-602.
[4] Gwyther, D., 2004. Impact of the deposition of jarosite on deepwater meiobenthos in southeastern. Australia Marine Pollution Bulletin, 48(9-10):997-1003.
[5] Heip, C., Smol, N., Hautekiet, W., 1974. A rapid method for extracting meiobenthic Nematodes and Copepods from mud and detritus. Marine Biology, 28:79-81.
[6] Jensen, P., 1982. A new meiofauna sample splitter. Annales Zoologici Fennici, 19:233-236.
[7] Kitazato, H., Nakatsuka, T., Shimanaga, M., Kanda, J., Soh, W., Kato, Y., Okada, Y., Yamaoka, A., Masuzawa, T., Suzuki, K., Shirayama, Y., 2003. Long-term monitoring of the sedimentary processes in the central part of Sagami Bay, Japan: Rationale, logistics and overview of results. Progress In Oceanography, 57(1):3-16.
[8] Kopp, M.U., Crabtree, H.J., Manz, A., 1997. Developments in technology and applications of Microsystems. Current Opinion in Chemical Biology, 1(3):410-419.
[9] Larkin, J.M., Donaldson, W.R., Foster, T.H., Knox, R.S., 1999. Reverse intersystem crossing from a triplet state of rose Bengal populated by sequential 532 nm plus 1064 nm laser excitation. Chemical Physics, 244:319-330.
[10] Li, Y.B., Zhu, J., Yang, Y.L., Xu, J., Zhang, H.G., Shi, H.M., 1996. The establishment of laser-induce fluorescence detection system. Laser Medicine, 6(3):97-102.
[11] Lu, X., Huang, W.H., Wang, Z.L., Cheng, J.K., 2004. Recent developments in single-cell analysis. Analytica Chimica Acta, 510(2):127-138.
[12] Mclntyre, A.D., 1969. Ecology of marine meiobenthos. Biological Reviews, 44:245-290.
[13] Michael, A., Daniel, R., Armstrong, W., 2004. Separation and analysis of colloidal/nano-particles including microorganisms by capillary electrophoresis: A fundamental review. Journal of Chromatography B, 800(1-2):7-25.
[14] Nichols, J.A., 1979. A simple flotation technique for separation meiobenthic nematodes from fine-grained sediments. Transactions of American Microscopical Society, 98:127-130.
[15] Schwinghamer, P., 1981. Extraction of living meiofauna from marine sediments by centrifugation in a silica Sol-Sorbitol mixture. Canadian Journal of Fisheries and Aquatic Sciences, 38:476-478.
Open peer comments: Debate/Discuss/Question/Opinion
<1>