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Received: 2005-06-10

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Journal of Zhejiang University SCIENCE B 2005 Vol.6 No.12 P.1188-1194


Infusion of nonmyeloablative bone marrow alleviates acute rejection reaction in liver allotransplantation

Author(s):  XIE Hai-yang, HUANG Dong-sheng, JIA Chang-ku, ZHENG Shu-sen

Affiliation(s):  Department of Hepatobiliary Pancreatic Surgery, Key Lab of Combined Multi-Organ Transplantation, Ministry of Public Health, First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310003, China

Corresponding email(s):   Zhengss@mail.hz.zj.cn

Key Words:  Liver transplantation, Rejection, Bone marrow infusion, Chimerism

XIE Hai-yang, HUANG Dong-sheng, JIA Chang-ku, ZHENG Shu-sen. Infusion of nonmyeloablative bone marrow alleviates acute rejection reaction in liver allotransplantation[J]. Journal of Zhejiang University Science B, 2005, 6(12): 1188-1194.

@article{title="Infusion of nonmyeloablative bone marrow alleviates acute rejection reaction in liver allotransplantation",
author="XIE Hai-yang, HUANG Dong-sheng, JIA Chang-ku, ZHENG Shu-sen",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Infusion of nonmyeloablative bone marrow alleviates acute rejection reaction in liver allotransplantation
%A XIE Hai-yang
%A HUANG Dong-sheng
%A JIA Chang-ku
%A ZHENG Shu-sen
%J Journal of Zhejiang University SCIENCE B
%V 6
%N 12
%P 1188-1194
%@ 1673-1581
%D 2005
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.2005.B1188

T1 - Infusion of nonmyeloablative bone marrow alleviates acute rejection reaction in liver allotransplantation
A1 - XIE Hai-yang
A1 - HUANG Dong-sheng
A1 - JIA Chang-ku
A1 - ZHENG Shu-sen
J0 - Journal of Zhejiang University Science B
VL - 6
IS - 12
SP - 1188
EP - 1194
%@ 1673-1581
Y1 - 2005
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.2005.B1188

Objective: To study the effect and implication of nonmyeloablative donor specific bone marrow (DSBM) infusion on the immunoreaction of liver allotransplantation. Methods: Orthotopic liver transplantation model was used in this study. Groups were set as follows: Group I, syngeneic control (Wistar-to-Wistar); Group II, acute rejection (SD-to-Wistar); Group III, acute rejection treated with cyclosporine A (CsA) by intramuscular injection (SD-to-Wistar+CsA); Group IV, bone marrow infusion at 7 d pretransplantation followed by short-term CsA treatment (SD-to-Wistar+DSBM); Another group of short-term CsA treatment preoperatively without bone marrow infusion was also set as control. General characteristics and survival time were observed. Histological grades of rejection were determined by pathological examination. IL-2 and IFN-γ level in peripheral blood and donor liver were detected respectively by Enzyme-Linked Immuno-Sorbent Assay (ELISA) and Western blot. chimerism of donor cells was measured by PCR for a male-specific marker (Y-chromosome-specific sequence, Sry). Results: No signs of rejection were found in Group I. Acute rejection occurred in both Group II and the short-term CsA treated group. All the recipients died at (9~15) d posttransplantation with a median survival time of (10.7±0.5) d and (11.2±2.4) d, respectively. Only mild rejection could be seen in Group III. In Group IV, 4 out of 6 recipients had long-term survival (>100 d), the histological grade of rejection was significantly lower than that of Group II, so did the expression level of IL-2 and IFN-γ in both peripheral blood and grafted liver. Y-chromosome-specific sequence (Sry) of male SD rats could be detected in the bone marrow, spleen and thymus of female recipients at 15 d after bone marrow infusion. Conclusion: Mild preconditioning nonmyeloablative donor specific bone marrow infusion can enhance chimerism formation in recipients, alleviate the rejection of liver allotransplantation and prolong survival of liver allotransplantation.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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