Full Text:   <1871>

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CLC number: R446

On-line Access: 2019-06-06

Received: 2019-01-29

Revision Accepted: 2019-04-14

Crosschecked: 2019-05-23

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Journal of Zhejiang University SCIENCE B 2019 Vol.20 No.7 P.605-612


Exosome-derived galectin-9 may be a novel predictor of rejection and prognosis after liver transplantation

Author(s):  Ai-Bin Zhang, Yi-Fan Peng, Jun-Jun Jia, Yu Nie, Shi-Yu Zhang, Hai-Yang Xie, Lin Zhou, Shu-Sen Zheng

Affiliation(s):  Division of Hepatobiliary and Pancreatic Surgery, Department of Surgery, the First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310003, China; more

Corresponding email(s):   shusenzheng@zju.edu.cn

Key Words:  Liver transplantation, Acute cellular rejection, Exosome, Galectin-9

Ai-Bin Zhang, Yi-Fan Peng, Jun-Jun Jia, Yu Nie, Shi-Yu Zhang, Hai-Yang Xie, Lin Zhou, Shu-Sen Zheng. Exosome-derived galectin-9 may be a novel predictor of rejection and prognosis after liver transplantation[J]. Journal of Zhejiang University Science B, 2019, 20(7): 605-612.

@article{title="Exosome-derived galectin-9 may be a novel predictor of rejection and prognosis after liver transplantation",
author="Ai-Bin Zhang, Yi-Fan Peng, Jun-Jun Jia, Yu Nie, Shi-Yu Zhang, Hai-Yang Xie, Lin Zhou, Shu-Sen Zheng",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Exosome-derived galectin-9 may be a novel predictor of rejection and prognosis after liver transplantation
%A Ai-Bin Zhang
%A Yi-Fan Peng
%A Jun-Jun Jia
%A Yu Nie
%A Shi-Yu Zhang
%A Hai-Yang Xie
%A Lin Zhou
%A Shu-Sen Zheng
%J Journal of Zhejiang University SCIENCE B
%V 20
%N 7
%P 605-612
%@ 1673-1581
%D 2019
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1900051

T1 - Exosome-derived galectin-9 may be a novel predictor of rejection and prognosis after liver transplantation
A1 - Ai-Bin Zhang
A1 - Yi-Fan Peng
A1 - Jun-Jun Jia
A1 - Yu Nie
A1 - Shi-Yu Zhang
A1 - Hai-Yang Xie
A1 - Lin Zhou
A1 - Shu-Sen Zheng
J0 - Journal of Zhejiang University Science B
VL - 20
IS - 7
SP - 605
EP - 612
%@ 1673-1581
Y1 - 2019
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1900051

acute cellular rejection (ACR) remains a major concern after liver transplantation. Predicting and monitoring acute rejection by non-invasive methods are very important for guiding the use of immunosuppressive drugs. Many studies have shown that exosomes and their contents are potential biomarkers for various liver diseases. Here, we identify and validate the role of exosomes and galectin-9 in ACR after liver transplantation. exosomes were isolated from three sets of paired patients, with and without ACR, and the proteins within the exosomes were isolated and identified. Candidate proteins were then validated using a tissue microarray containing resected liver samples from 73 ACR and 63 non-rejection patients. Finally, protein expression and clinical manifestations were included in Kaplan-Meier survival and Cox regression analyses. Circulating exosomes were isolated from ACR and non-rejection patients and characterized using transmission electron microscopy and western blotting for CD63/CD81. Western blotting experiments revealed higher levels of galectin-9 protein in circulating exosomes from ACR recipients. Immunohistochemical analysis of the tissue microarray showed that the expression of galectin-9 in resected liver was significantly higher in the ACR group than in the non-rejection group (P<0.05). Higher levels of galectin-9 expression in resected livers were associated with poorer prognosis (P<0.05). exosome-derived galectin-9 may be a novel predictor of rejection and prognosis after liver transplantation.




Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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