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CLC number: R319

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Received: 2005-02-26

Revision Accepted: 2005-08-19

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Cited: 6

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Journal of Zhejiang University SCIENCE B 2005 Vol.6 No.12 P.1210-1212

http://doi.org/10.1631/jzus.2005.B1210


A novel technique of three-dimensional reconstruction segmentation and analysis for sliced images of biological tissues


Author(s):  LI Jing, ZHAO Hai-yan, RUAN Xing-yun, XU Yong-qing, MENG Wei-zheng, LI Kun-peng, ZHANG Jing-qiang

Affiliation(s):  Department of Equipments, Kunming General Hospital, Kunming 650032, China; more

Corresponding email(s):   li_jingkm@126.com

Key Words:  Sliced images, 3D reconstruction and analysis, 3D segmentation, Chaperonin, Virus


LI Jing, ZHAO Hai-yan, RUAN Xing-yun, XU Yong-qing, MENG Wei-zheng, LI Kun-peng, ZHANG Jing-qiang. A novel technique of three-dimensional reconstruction segmentation and analysis for sliced images of biological tissues[J]. Journal of Zhejiang University Science B, 2005, 6(12): 1210-1212.

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author="LI Jing, ZHAO Hai-yan, RUAN Xing-yun, XU Yong-qing, MENG Wei-zheng, LI Kun-peng, ZHANG Jing-qiang",
journal="Journal of Zhejiang University Science B",
volume="6",
number="12",
pages="1210-1212",
year="2005",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.2005.B1210"
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%T A novel technique of three-dimensional reconstruction segmentation and analysis for sliced images of biological tissues
%A LI Jing
%A ZHAO Hai-yan
%A RUAN Xing-yun
%A XU Yong-qing
%A MENG Wei-zheng
%A LI Kun-peng
%A ZHANG Jing-qiang
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A1 - LI Jing
A1 - ZHAO Hai-yan
A1 - RUAN Xing-yun
A1 - XU Yong-qing
A1 - MENG Wei-zheng
A1 - LI Kun-peng
A1 - ZHANG Jing-qiang
J0 - Journal of Zhejiang University Science B
VL - 6
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SP - 1210
EP - 1212
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PB - Zhejiang University Press & Springer
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DOI - 10.1631/jzus.2005.B1210


Abstract: 
A novel technique of three-dimensional (3D) reconstruction, segmentation, display and analysis of series slices of images including microscopic wide field optical sectioning by deconvolution method, cryo-electron microscope slices by Fourier-Bessel synthesis and electron tomography (ET), and a series of computed tomography (CT) was developed to perform simultaneous measurement on the structure and function of biomedical samples. The paper presents the 3D reconstruction segmentation display and analysis results of pollen spore, chaperonin, virus, head, cervical bone, tibia and carpus. At the same time, it also puts forward some potential applications of the new technique in the biomedical realm.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

Reference

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[6] Nitsch, M., Walz, J., Typke, D., Klumpp, M., Essen, L.O., Baumeister, W., 1998. Group II chaperonin in an open conformation examined by electron tomography. Nature Structural Biology, 5(10):855-859.

[7] Paddock, S., 2002. Optical sectioning−slices of life. Science, 295:1319-1321.

[8] Sharpe, J., Ahlgren, U., Perry, P., Hill, B., Ross, A., Hecksher-Sorensen, J., Baldock, R., Davidson, D., 2002. Optical projection tomography as a tool for 3D microscopy and gene expression studies. Science, 296:541-545.

[9] Trehan, G., Gauvrit, J.Y., Leclerc, X., 2003. Intracranial aneurysms: diagnostic and therapeutic contribution of rotational brain arteriography with 3D reconstruction. Rev. Neurol. (Paris), 159(3):340-345.

[10] Yelbuz, T.M., Choma, M.A., Thrane, L., Kirby, M.L., Izatt, J.A., 2002. Optical coherence tomography: a new high-resolution imaging technology to study cardiac development in chick embryos. Circulation, 106(22):2771-2777.

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