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Journal of Zhejiang University SCIENCE B 2006 Vol.7 No.5 P.373-376

http://doi.org/10.1631/jzus.2006.B0373


Functional analysis of helicase and three tandem HRDC domains of RecQ in Deinococcus radiodurans


Author(s):  Huang Li-fen, Hua Xiao-ting, Lu Hui-ming, Gao Guan-jun, Tian Bing, Shen Bing-hui, Hua Yue-jin

Affiliation(s):  Institute of Nuclear-Agricultural Science, Zhejiang University, Hangzhou 310029, China; more

Corresponding email(s):   yjhua@zju.edu.cn

Key Words:  RecQ, Deinococcus radiodurans, Helicase, HRDC


Huang Li-fen, Hua Xiao-ting, Lu Hui-ming, Gao Guan-jun, Tian Bing, Shen Bing-hui, Hua Yue-jin. Functional analysis of helicase and three tandem HRDC domains of RecQ in Deinococcus radiodurans[J]. Journal of Zhejiang University Science B, 2006, 7(5): 373-376.

@article{title="Functional analysis of helicase and three tandem HRDC domains of RecQ in Deinococcus radiodurans",
author="Huang Li-fen, Hua Xiao-ting, Lu Hui-ming, Gao Guan-jun, Tian Bing, Shen Bing-hui, Hua Yue-jin",
journal="Journal of Zhejiang University Science B",
volume="7",
number="5",
pages="373-376",
year="2006",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.2006.B0373"
}

%0 Journal Article
%T Functional analysis of helicase and three tandem HRDC domains of RecQ in Deinococcus radiodurans
%A Huang Li-fen
%A Hua Xiao-ting
%A Lu Hui-ming
%A Gao Guan-jun
%A Tian Bing
%A Shen Bing-hui
%A Hua Yue-jin
%J Journal of Zhejiang University SCIENCE B
%V 7
%N 5
%P 373-376
%@ 1673-1581
%D 2006
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.2006.B0373

TY - JOUR
T1 - Functional analysis of helicase and three tandem HRDC domains of RecQ in Deinococcus radiodurans
A1 - Huang Li-fen
A1 - Hua Xiao-ting
A1 - Lu Hui-ming
A1 - Gao Guan-jun
A1 - Tian Bing
A1 - Shen Bing-hui
A1 - Hua Yue-jin
J0 - Journal of Zhejiang University Science B
VL - 7
IS - 5
SP - 373
EP - 376
%@ 1673-1581
Y1 - 2006
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.2006.B0373


Abstract: 
recQ is a highly conserved helicase necessary for maintaining genome stability in all organisms. Genome comparison showed that a homologue of recQ in Deinococcus radiodurans designated as DR1289 is a member of recQ family with unusual domain arrangement: a helicase domain, an recQ C-terminal domain, and surprisingly three HRDC domain repeats, whose function, however, remains obscure currently. Using an insertion deletion, we discovered that the DRrecQ mutation causes an increase in gamma radiation, hydroxyurea and mitomycine C and UV sensitivity. Using the shuttle plasmid pRADK, we complemented various domains of the D. radiodurans recQ (DRrecQ) to the mutant in vivo. Results suggested that both the helicase and helicase-and-RNase-D-C-terminal (HRDC) domains are essential for complementing several phenotypes. The complementation and biochemical function of DRrecQ variants with different domains truncated in vitro suggested that both the helicase and three HRDC domains are necessary for recQ functions in D. radiodurans, while three HRDC domains have a synergistic effect on the whole function. Our finding leads to the hypothesis that the RecF recombination pathway is likely a primary path of double strand break repair in this well-known radioresistant organism.

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Reference

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[7] Minton, K.W., 1994. DNA repair in the extremely radioresistant bacterium Deinococcus radiodurans. Mol. Microbiol., 13(1):9-15.

[8] Morozov, V., Mushegian, A.R., Koonin, E.V., Bork, P., 1997. A putative nucleic acid-binding domain in Bloom’s and Werner’s syndrome helicases. Trends Biochem. Sci., 22(11):417-418.

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[10] White, O., Eisen, J.A., Heidelberg, J.F., Hickey, E.K., Peterson, J.D., Dodson, R.J., Haft, D.H., Gwinn, M.L., Nelson, W.C., Richardson, D.L., et al., 1999. Genome sequence of the radioresistant bacterium Deinococcus radiodurans R1. Science, 286(5444):1571-1577.

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