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Received: 2005-08-10

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Journal of Zhejiang University SCIENCE B 2006 Vol.7 No.6 P.452-458


Binding interactions of pefloxacin mesylate with bovine lactoferrin and human serum albumin

Author(s):  FAN Ji-cai, CHEN Xiang, WANG Yun, FAN Cheng-ping, SHANG Zhi-cai

Affiliation(s):  Department of Chemistry, Zhejiang University, Hangzhou 310027, China

Corresponding email(s):   shangzc@mail.hz.zj.cn

Key Words:  Pefloxacin mesylate (PFLX), Bovine lactoferrin (BLf), Human serum albumin (HSA), Fluorescence spectra, Energy-transfer efficiency

FAN Ji-cai, CHEN Xiang, WANG Yun, FAN Cheng-ping, SHANG Zhi-cai. Binding interactions of pefloxacin mesylate with bovine lactoferrin and human serum albumin[J]. Journal of Zhejiang University Science B, 2006, 7(6): 452-458.

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publisher="Zhejiang University Press & Springer",

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%A FAN Ji-cai
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%A FAN Cheng-ping
%A SHANG Zhi-cai
%J Journal of Zhejiang University SCIENCE B
%V 7
%N 6
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%@ 1673-1581
%D 2006
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.2006.B0452

T1 - Binding interactions of pefloxacin mesylate with bovine lactoferrin and human serum albumin
A1 - FAN Ji-cai
A1 - CHEN Xiang
A1 - WANG Yun
A1 - FAN Cheng-ping
A1 - SHANG Zhi-cai
J0 - Journal of Zhejiang University Science B
VL - 7
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SP - 452
EP - 458
%@ 1673-1581
Y1 - 2006
PB - Zhejiang University Press & Springer
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DOI - 10.1631/jzus.2006.B0452

The binding of pefloxacin mesylate (PFLX) to bovine lactoferrin (BLf) and human serum albumin (HSA) in dilute aqueous solution was studied using fluorescence spectra and absorbance spectra. The binding constant K and the binding sites n were obtained by fluorescence quenching method. The binding distance r and energy-transfer efficiency E between pefloxacin mesylate and bovine lactoferrin as well as human serum albumin were also obtained according to the mechanism of Förster-type dipole-dipole nonradiative energy-transfer. The effects of pefloxacin mesylate on the conformations of bovine lactoferrin and human serum albumin were also analyzed using synchronous fluorescence spectroscopy.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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