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On-line Access: 2016-06-03

Received: 2015-11-28

Revision Accepted: 2016-02-02

Crosschecked: 2016-05-12

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Li-rong Shen


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Journal of Zhejiang University SCIENCE B 2016 Vol.17 No.6 P.476-483


Evaluation of the major royal jelly proteins as an alternative to fetal bovine serum in culturing human cell lines

Author(s):  Di Chen, Xiao-xuan Xin, Hao-cheng Qian, Zhang-yin Yu, Li-rong Shen

Affiliation(s):  Department of Food Science and Nutrition, Fuli Institute of Food Science, Zhejiang Key Laboratory for Agro-Food Processing, Zhejiang University, Hangzhou 310058, China

Corresponding email(s):   shenlirong@zju.edu.cn

Key Words:  Major royal jelly proteins (MRJPs), Cell culture, Alternative, Fetal bovine serum (FBS), Cytokine

Di Chen, Xiao-xuan Xin, Hao-cheng Qian, Zhang-yin Yu, Li-rong Shen. Evaluation of the major royal jelly proteins as an alternative to fetal bovine serum in culturing human cell lines[J]. Journal of Zhejiang University Science B, 2016, 17(6): 476-483.

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author="Di Chen, Xiao-xuan Xin, Hao-cheng Qian, Zhang-yin Yu, Li-rong Shen",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Evaluation of the major royal jelly proteins as an alternative to fetal bovine serum in culturing human cell lines
%A Di Chen
%A Xiao-xuan Xin
%A Hao-cheng Qian
%A Zhang-yin Yu
%A Li-rong Shen
%J Journal of Zhejiang University SCIENCE B
%V 17
%N 6
%P 476-483
%@ 1673-1581
%D 2016
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1500295

T1 - Evaluation of the major royal jelly proteins as an alternative to fetal bovine serum in culturing human cell lines
A1 - Di Chen
A1 - Xiao-xuan Xin
A1 - Hao-cheng Qian
A1 - Zhang-yin Yu
A1 - Li-rong Shen
J0 - Journal of Zhejiang University Science B
VL - 17
IS - 6
SP - 476
EP - 483
%@ 1673-1581
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PB - Zhejiang University Press & Springer
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DOI - 10.1631/jzus.B1500295

Royal jelly (RJ) is a well-known bioactive substance. It contains large amounts of major royal jelly proteins (MRJPs), which express growth-factor-like activity in several animal and human cell lines. However, the question on whether MRJPs possess growth-factor-like activity on all types of cell cultures remains. In order to determine whether MRJPs can be used as an alternative to fetal bovine serum (FBS) in different types of human cell culture, the proliferation of the complex serum with different ratios of MRJPs/FBS (M/F) was evaluated on five cell lines: 293T, HFL-I, 231, HCT116, and Changliver using MTT (3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide) assay. The proliferation activity of the combination of the complex M/F serum with cytokines on the test cell lines was also measured. The results demonstrated that the complex serum with M/F 6/4 possessed the highest proliferation activity similar to or in excess of FBS. However, no activity of complex medium with M/F 6/4 was observed in 231 cells, indicating a selectivity of MRJPs on cell types. Compared with the complex medium with M/F 6/4, the complex medium with M/F 6/4 together with two cytokines, epidermal growth factor (EGF) and insulin-transferrin-selenium (ITS), promoted proliferations of Changliver, 293T, HCT116, and HFL-I by 18.73%‒56.19% (P<0.01). Our findings demonstrate that MRJPs could partially replace FBS in culturing many human cell lines.


结论:MRJPs对多种人体细胞具有促进分裂作用,可部分替代FBS培养人体细胞。MRJPs与FBS的最佳配比为:60% MRJPs溶液和40% FBS(复合血清);该复合血清与细胞因子的最佳组合为:复合血清+表皮生长因子+胰岛素-转铁蛋白-硒。

关键词:蜂王浆主蛋白;灭活胎牛血清;细胞培养基;替代品;分裂; 细胞因子

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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