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Journal of Zhejiang University SCIENCE B 2006 Vol.7 No.9 P.749-756

http://doi.org/10.1631/jzus.2006.B0749


Edaravone protects PC12 cells from ischemic-like injury via attenuating the damage to mitochondria


Author(s):  SONG Ying, LI Meng, LI Ji-cheng, WEI Er-qing

Affiliation(s):  Department of Cellular Biology, School of Medicine, Zhejiang University, Hangzhou 310031, China; more

Corresponding email(s):   weieq2001@yahoo.com

Key Words:  Edaravone, Ischemia, Apoptosis, Rat pheochromocytoma (PC12) cells, Mitochondria, Bax, Bcl-2, Oxygen-glucose deprivation (OGD)


SONG Ying, LI Meng, LI Ji-cheng, WEI Er-qing. Edaravone protects PC12 cells from ischemic-like injury via attenuating the damage to mitochondria[J]. Journal of Zhejiang University Science B, 2006, 7(9): 749-756.

@article{title="Edaravone protects PC12 cells from ischemic-like injury via attenuating the damage to mitochondria",
author="SONG Ying, LI Meng, LI Ji-cheng, WEI Er-qing",
journal="Journal of Zhejiang University Science B",
volume="7",
number="9",
pages="749-756",
year="2006",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.2006.B0749"
}

%0 Journal Article
%T Edaravone protects PC12 cells from ischemic-like injury via attenuating the damage to mitochondria
%A SONG Ying
%A LI Meng
%A LI Ji-cheng
%A WEI Er-qing
%J Journal of Zhejiang University SCIENCE B
%V 7
%N 9
%P 749-756
%@ 1673-1581
%D 2006
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.2006.B0749

TY - JOUR
T1 - Edaravone protects PC12 cells from ischemic-like injury via attenuating the damage to mitochondria
A1 - SONG Ying
A1 - LI Meng
A1 - LI Ji-cheng
A1 - WEI Er-qing
J0 - Journal of Zhejiang University Science B
VL - 7
IS - 9
SP - 749
EP - 756
%@ 1673-1581
Y1 - 2006
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.2006.B0749


Abstract: 
Background: edaravone had been validated to effectively protect against ischemic injuries. In this study, we investigated the protective effect of edaravone by observing the effects on anti-apoptosis, regulation of bcl-2/bax protein expression and recovering from damage to mitochondria after OGD (oxygen-glucose deprivation)-reperfusion. Methods: Viability of PC12 cells which were injured at different time of OGD injury, was quantified by measuring MTT (2-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) staining. In addition, PC12 cells’ viability was also quantified after their preincubation in different concentration of edaravone for 30 min followed by (OGD). Furthermore, apoptotic population of PC12 cells that reinsulted from OGD-reperfusion with or without preincubation with edaravone was determined by flow cytometer analysis, electron microscope and Hoechst/PI staining. Finally, change of bcl-2/bax protein expression was detected by Western blot. Results: (1) The viability of PC12 cells decreased with time (1~12 h) after OGD. We regarded the model of OGD 2 h, then replacing DMEM (Dulbecco’s Modified Eagle’s Medium) for another 24 h as an OGD-reperfusion in this research. Furthermore, most PC12 cells were in the state of apoptosis after OGD-reperfusion. (2) The viability of PC12 cells preincubated with edaravone at high concentrations (1, 0.1, 0.01 μmol/L) increased significantly with edaravone protecting PC12 cells from apoptosis after OGD-reperfusion injury. (3) Furthermore, edaravone attenuates the damage of OGD-reperfusion on mitochondria and regulated bcl-2/bax protein imbalance expression after OGD-reperfusion. Conclusion: Neuroprotective effects of edaravone on ischemic or other brain injuries may be partly mediated through inhibition of bcl-2/bax apoptotic pathways by recovering from the damage of mitochondria.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

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